dc.contributor.author | Sezgintürk, Mustafa Kemal | |
dc.contributor.author | Dinçkaya, Erhan | |
dc.date.accessioned | 2022-05-11T14:29:43Z | |
dc.date.available | 2022-05-11T14:29:43Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1073-1199 | |
dc.identifier.uri | https://doi.org/10.3109/10731199.2011.560119 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/7108 | |
dc.description.abstract | beta-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor beta-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. beta-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for beta-galactosidase detection was in the range of 0.0118 U mL(-1) to 0.47 U mL(-1) and a shorter response time of similar to 50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of beta-galactosidase activity. | en_US |
dc.description.sponsorship | Ege UniversityEge University; Scientific Research Foundation [2004 FEN 002]; Scientific and Technical Research Council of Turkiye (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) | en_US |
dc.description.sponsorship | The authors are grateful for the financial support of Ege University, Scientific Research Foundation (Grant No. 2004 FEN 002) and The Scientific and Technical Research Council of Turkiye (TUBITAK). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Informa Healthcare | en_US |
dc.identifier.doi | 10.3109/10731199.2011.560119 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | beta-galactosidase | en_US |
dc.subject | glucose oxidase | en_US |
dc.subject | biosensor | en_US |
dc.subject | enzyme activity | en_US |
dc.subject | electropolymerization | en_US |
dc.subject | Alkaline-Phosphatase | en_US |
dc.subject | Escherichia-Coli | en_US |
dc.subject | In-Vivo | en_US |
dc.subject | Surfaces | en_US |
dc.subject | Enzyme | en_US |
dc.subject | Label | en_US |
dc.subject | Water | en_US |
dc.title | A Biosensor for the Determination of beta-galactosidase Activity: A Different Viewpoint on Biosensors | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Artificial Cells Blood Substitutes and Biotechnology | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.identifier.volume | 39 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.startpage | 281 | en_US |
dc.identifier.endpage | 288 | en_US |
dc.institutionauthor | Sezgintürk, Mustafa Kema | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 6506764918 | |
dc.authorscopusid | 6603789064 | |
dc.authorwosid | DİNÇKAYA, ERHAN/AAG-7096-2021 | |
dc.authorwosid | Sezgintürk, Mustafa Kemal/AAY-1439-2021 | |
dc.identifier.wos | WOS:000294740300003 | en_US |
dc.identifier.scopus | 2-s2.0-80052751993 | en_US |
dc.identifier.pmid | 21351849 | en_US |