dc.contributor.author | Öncü, Ceren | |
dc.contributor.author | Brinkmann, Annika | |
dc.contributor.author | Günay, Filiz | |
dc.contributor.author | Kar, Sırrı | |
dc.contributor.author | Öter, Kerem | |
dc.contributor.author | Sarıkaya, Yasemen | |
dc.contributor.author | Ergünay, Koray | |
dc.date.accessioned | 2022-05-11T14:28:39Z | |
dc.date.available | 2022-05-11T14:28:39Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 1567-1348 | |
dc.identifier.issn | 1567-7257 | |
dc.identifier.uri | https://doi.org/10.1016/j.meegid.2017.11.003 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/6903 | |
dc.description.abstract | Mosquitoes are involved in the transmission and maintenance of several viral diseases with significant health impact. Biosurveillance efforts have also revealed insect-specific viruses, observed to cocirculate with pathogenic strains. This report describes the findings of flavivirus and rhabdovirus screening, performed in eastern Thrace and Aegean region of Anatolia during 2016, including and expanding on locations with previously-documented virus activity. A mosquito cohort of 1545 individuals comprising 14 species were collected and screened in 108 pools via generic and specific amplification and direct metagenomics by next generation sequencing. Seven mosquito pools (6.4%) were positive in the flavivirus screening. West Nile virus lineage 1 clade 1a sequences were characterized in a pool Culex pipiens sensu lato specimens, providing the initial virus detection in Aegean region following 2010 outbreak. In an Anopheles maculipennis sensu lato pool, sequences closely-related to Anopheles flaviviruses were obtained, with similarities to several African and Australian strains of this new insect-specific flavivirus clade. In pools comprising Uranotaenia unguiculata (n = 3), Cx. pipiens s. l. (n = 1) and Aedes caspius (n = 1) mosquitoes, sequences of a novel flavivirus, distantly-related to Flavivirus AV2011, identified previously in Spain and Turkey, were characterized. Moreover, DNA forms of the novel flavivirus were detected in two Ur. unguiculata pools. These sequences were highly-similar to the sequences amplified from viral RNA, with undisrupted reading frames, suggest the occurrence of viral DNA forms in natural conditions within mosquito hosts. Rhabdovirus screening revealed sequences of a recently-described novel virus, named the Merida-like virus Turkey (MERDLVT) in 5 Cx. pipiens s. l. pools (4.6%). Partial L and N gene sequences of MERDLVT were well-conserved among strains, with evidence for geographical clustering in phylogenetic analyses. Metagenomics provided the near-full genomic sequence in a specimen, revealing an identical genome organization and limited divergence from the prototype MERDLVT isolate. | en_US |
dc.description.sponsorship | Armed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System (AFHSC-GEIS), United States [W911QY-16-C-0160-P00001]; Georg Forster Research Fellowship (HERMES) for Experienced Researchers, of the Alexander von Humboldt Foundation; National Research Council (NRC) Research Associateship Award at the Walter Reed Army Institute of Research | en_US |
dc.description.sponsorship | This study was partially supported by The Armed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System (AFHSC-GEIS) (W911QY-16-C-0160-P00001), United States (with Yvonne Marie-Linton as the principal investigator). KE is a recipient of the Georg Forster Research Fellowship (HERMES) for Experienced Researchers, of the Alexander von Humboldt Foundation, 2015. This manuscript was prepared whilst YML held a National Research Council (NRC) Research Associateship Award at the Walter Reed Army Institute of Research. This research was performed in part under a Memorandum of Understanding between the Walter Reed Army Institute of Research and the Smithsonian Institution, with institutional support provided by both organizations. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The material to be published reflects the views of the authors and should not be construed to represent those of the US Department of the Army or the US Department of Defense. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.identifier.doi | 10.1016/j.meegid.2017.11.003 | |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | West Nile | en_US |
dc.subject | Flavivirus | en_US |
dc.subject | Rhabdovirus | en_US |
dc.subject | Anopheles | en_US |
dc.subject | Mosquito | en_US |
dc.subject | Turkey | en_US |
dc.subject | Insect-Specific Flaviviruses | en_US |
dc.subject | 1st Evidence | en_US |
dc.subject | Host-Range | en_US |
dc.subject | Lineage | en_US |
dc.subject | Circulation | en_US |
dc.subject | Alignment | en_US |
dc.subject | Reveals | en_US |
dc.subject | Surveillance | en_US |
dc.subject | Infections | en_US |
dc.subject | Emergence | en_US |
dc.title | West Nile virus, Anopheles flavivirus, a novel flavivirus as well as Merida-like rhabdovirus Turkey in field-collected mosquitoes from Thrace and Anatolia | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Infection Genetics and Evolution | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü | en_US |
dc.authorid | 0000-0002-2583-6264 | |
dc.authorid | 0000-0001-5422-1982 | |
dc.authorid | 0000-0001-8185-3176 | |
dc.authorid | 0000-0002-9711-4113 | |
dc.identifier.volume | 57 | en_US |
dc.identifier.startpage | 36 | en_US |
dc.identifier.endpage | 45 | en_US |
dc.institutionauthor | Kar, Sırrı | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 57188633392 | |
dc.authorscopusid | 55258915300 | |
dc.authorscopusid | 37114339500 | |
dc.authorscopusid | 15769247000 | |
dc.authorscopusid | 36968366100 | |
dc.authorscopusid | 57191475147 | |
dc.authorscopusid | 7004626809 | |
dc.authorwosid | Linton, Yvonne-Marie/AAW-3992-2021 | |
dc.authorwosid | Gunay, Filiz/AAC-7874-2020 | |
dc.authorwosid | Ergunay, Koray/I-8368-2013 | |
dc.authorwosid | Linton, Yvonne/AAG-2353-2019 | |
dc.identifier.wos | WOS:000417475600004 | en_US |
dc.identifier.scopus | 2-s2.0-85033436863 | en_US |
dc.identifier.pmid | 29128516 | en_US |