Baykara, S.G.Sürmeli, YusufŞanlı Mohamed, Gülşah2022-05-112022-05-1120210273-2289https://doi.org/10.1007/s12010-021-03512-0https://hdl.handle.net/20.500.11776/5007Proteases account for approximately 60% of the enzyme market in the world, and they are used in various industrial applications including the detergent industry. In this study, production and characterization of a novel serine protease of thermophilic Geobacillus sp. GS53 from Balçova geothermal region, İzmir, Turkey, were performed. The thermostable protease was purified through ammonium sulfate precipitation and anion-exchange chromatography. The results showed that the protease had 137.8 U mg?1 of specific activity and optimally worked at 55 oC and pH 8. It was also active in a broad pH (4–10) and temperature (25–75 °C) ranges. The protease was highly stable at 85 °C and demonstrated relative stability at pH 4, 7, and 10. Also, the enzyme had high stability against organic solvents and surfactants; enzyme relative activity did not decrease below 81% upon preincubation for 10 min. Ca2+, Cu2+, and Zn2+ ions slightly induced protease activity. The protease was highly specific to casein, skim milk, Hammerstein casein, and BSA substrates. These results revealed that the protease might have a potential effect in a variety of industrial fields, especially the detergent industry, because of its high thermostability and stability to surfactants. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.en10.1007/s12010-021-03512-0info:eu-repo/semantics/closedAccessGeobacillusSerine proteaseSurfactantThermostabilityAmino acidsCaseinDairiesNitrogen compoundsPurificationSulfur compoundsSurface active agentsAmmonium sulfate precipitationAnion exchange chromatographyBiochemical characterizationDetergent industryIndustrial fieldsProtease activitiesRelative activitiesRelative stabilitiesEnzyme activityalkaline proteinaseammonium sulfatecalcium ioncaseincuprous ionserine proteinasezinc ioncalciumcopperserine proteinasesurfactantzincanion exchange chromatographyArticlebacterial strainbiochemistrycontrolled studyenzyme activityenzyme purificationenzyme specificityenzyme stabilityGeobacillusGeobacillus stearothermophilusGeobacillus toebiiion exchange chromatographymolecular weightnonhumanpHpolyacrylamide gel electrophoresisprecipitationskim milktemperatureTurkey (republic)chemistryenzyme stabilityenzymologyGeobacillusmetabolismCalciumChromatography, Ion ExchangeCopperEnzyme StabilityGeobacillusHydrogen-Ion ConcentrationMolecular WeightSerine ProteasesSubstrate SpecificitySurface-Active AgentsZincArticle193515741584Q3WOS:0006125708000022-s2.0-8510056977933507494Q2