Aydın, Elif BurcuSezgintürk, Mustafa Kemal2022-05-112022-05-1120211567-5394https://doi.org/10.1016/j.bioelechem.2020.107698https://hdl.handle.net/20.500.11776/5104In this study, we utilized a carboxyalkylphosphonic acid covered fluorine doped tin oxide (FTO) as an electrode material for fabrication of Interleukin 1? (IL-1?) immunosensor. For this aim, anti-IL-1? antibodies were attached on the 3-phosphonopropionic acid (PHP) modified FTO surface covalently. Electrochemical (electrochemical impedance spectroscopy and cyclic voltammetry) and morphological (scanning electron microscopy and atomic force microscopy) characterizations were performed to monitor the successful fabrication of immunoelectrodes. After incubation of anti-IL-1? antibody immobilized FTO electrodes in IL-1? antigen solutions, increases were seen in impedimetric responses. IL-1? antigen was determined in a linear detection range from 0.02 to 2 pg/mL by EIS. The detection limit of the suggested immunosensor was 6 fg/mL. The applicability of the designed biosensor was tested by using human serum and saliva samples and acceptable results were obtained. In addition, high sensitivity, good specificity, low detection limit made the proposed immunosensor a potential technique for IL-1? antigen determination in routine clinical analysis. © 2020 Elsevier B.V.en10.1016/j.bioelechem.2020.107698info:eu-repo/semantics/closedAccess3-Phosphonopropionic acidFluorine doped tin oxideImpedimetric biosensorInterleukin-1?AntibodiesAntigensAtomic force microscopyElectrochemical impedance spectroscopyElectrodesFabricationImmunosensorsNanocompositesScanning electron microscopyTin oxidesClinical analysisDisposable biosensorElectrode materialFluorine doped tin oxideLinear detection rangesLow detection limitPotential techniquesUltrasensitive detectionCyclic voltammetry3 phosphonopropionic acidfluorineimmobilized antibodyinterleukin 1alphapropionic acid derivativetin oxideunclassified drug2-phosphonopropionic acidfluorineinterleukin 1alphaphosphonic acid derivativepropionic acid derivativestannic oxidetin derivativeArticleatomic force microscopyclinical evaluationcovalent bondcyclic voltammetryelectrochemical analysishumanimpedance spectroscopylimit of detectionmeasurement repeatabilityreference electrodereproducibilitysalivascanning electron microscopysensitivity and specificityserumbloodchemistrydevicesdisposable equipmentgenetic proceduressurface propertyAntibodies, ImmobilizedBiosensing TechniquesDisposable EquipmentFluorineHumansInterleukin-1alphaLimit of DetectionOrganophosphonatesPropionatesSalivaSurface PropertiesTin CompoundsArticle138Q1WOS:0006635338000172-s2.0-8509686071033254051Q2