Karaarslan, NumanYılmaz, İbrahimŞirin, Duygu YaşarBaykız, DeryaDemirkiran, AykutAteş, Özkan2022-05-112022-05-1120182636-76882636-7688https://doi.org/10.5455/jtomc.2018.04.063https://app.trdizin.gov.tr/makale/TWpnMU5UVTJOZz09https://hdl.handle.net/20.500.11776/5455Aim: Nimodipine is used to prevent cerebrovascular-originated ischemic neurological deficits, yet its effects on nucleus pulposus (NP) cells or annulus fibrosus (AF) cells weren’t studied. This study aimed to examine nimodipine’s effects on vitality and proliferation of chondroadherin (CHAD), type II collagen (COL2A1), and hypoxia-inducible factor 1 alpha (HIF 1?) gene expression in human primary NP/AF cells.Material and Methods: NP/AF cell cultures obtained from 6 patients who underwent microdiscectomy were treated with 100 µMolar nimodipine and analyzed at 0, 24, and 48 h. Data were evaluated using one-way ANOVA and post-hoc Tukey HSD with 95% confidence interval.Results: We observed suppressed cell proliferation and increased necrosis in nimodipine-treated NP/AF cell cultures, especially degenerated tissue. COL2A1 gene expression wasn’t detected in any experimental groups. CHAD and HIF 1? expression had timedependent decreases in control. CHAD and HIF 1? expression were found to decrease at 24h, but increased at 48h in degenerated tissue. In nimodipine-applied intact tissues, CHAD expression was stable at 24h but 1.62 times higher than control at 48h. HIF 1? levels were lower than control.Conclusion: In nimodipine-treated degenerated AF/NP cultures, CHAD and HIF 1? expressions had time-dependent decreases. However, after complete RT-PCR data evaluation, no correlation between nimodipine application and gene expression occurred.en10.5455/jtomc.2018.04.063info:eu-repo/semantics/openAccessBiyolojiAlerjiAnatomi ve MorfolojiAndrolojiAnesteziOdyoloji ve Konuşma-Dil PatolojisiBiyokimya ve Moleküler BiyolojiBiyofizikBiyoteknoloji ve Uygulamalı MikrobiyolojiKalp ve Kalp Damar SistemiHücre BiyolojisiKlinik NörolojiYoğun BakımTıpDermatolojiAcil TıpEndokrinoloji ve MetabolizmaGastroenteroloji ve HepatolojiGeriatri ve GerontolojiSağlık Bilimleri ve HizmetleriHematolojiİmmünolojiEnfeksiyon HastalıklarıTamamlayıcı ve Entegre TıpTıbbi EtikTıbbi İnformatikTıbbi Laboratuar TeknolojisiGenel ve Dahili TıpAdli TıpTıbbi Araştırmalar DeneyselMikrobiyolojiNörolojik BilimlerKadın Hastalıkları ve DoğumOnkolojiGöz HastalıklarıOrtopediKulakBurunBoğazPatolojiPediatriPeriferik Damar HastalıklarıFizyolojiTemel Sağlık HizmetleriPsikiyatriHalk ve Çevre SağlığıRadyolojiNükleer TıpTıbbi GörüntülemeRehabilitasyonSolunum SistemiRomatolojiSpor BilimleriCerrahiTransplantasyonTropik TıpÜroloji ve NefrolojiArticle252266273TWpnMU5UVTJOZz09