Bilgen, Behiye BanuYuruk, BusraNasirian, Hazal2024-10-292024-10-2920231300-011X1303-6173https://doi.org/10.55730/1300-011X.3127https://search.trdizin.gov.tr/tr/yayin/detay/1208202https://hdl.handle.net/20.500.11776/14840Triticum aestivum L. is one of the major strategic crops for many reasons such as being the basic material for the majority of nutrients, being a source of energy, a source of protein, and also other organic and inorganic compounds for both humans and animals. The genetic characterization is useful for designing plant breeding programs for many plants, including wheat. The selection of genotypes resistant to stress (biotic and abiotic) conditions is another important point for the design of plant breeding programs. Wheat brown (leaf) rust is one of the major significant biotic stress factors. In this study, marker analysis for brown rust resistance for Lr13, Lr14a, Lr19, Lr22a, Lr24, Lr34, Lr37, and Lr47 genes was performed, and the genetic characterization of 96 wheat genotypes was determined by 8 SSR and 4 ISSR loci. Eighteen alleles were determined for SSR loci (polymorphism ratio of 37.5%) and a total of 33 polymorphic bands (polymorphism ratio of 82.5%) were determined for ISSR loci. The mean Shannon Index was estimated as I (SSR) = 0.298 and I (ISSR) = 0.346. The mean gene diversity for SSR and ISSR was estimated as 0.407 and 0.246, respectively. The genetic similarity value based unweighted pair group method with arithmetic was used to form the dendrogram and it was remarked wheat varieties were grouped into two main clusters. The STRUCTURE analysis also separated the studied wheat genotypes into two groups. The results reveal the genetic structure, the genetic diversity, and the presence of resistance alleles of used wheat varieties, and the selection of genetically diverse and leaf rust-resistant genotypes can serve as valuable parents for future wheat breeding or improving breeding programs.en10.55730/1300-011X.3127info:eu-repo/semantics/openAccessBreedinggenetic diversityPuccinia triticinaSSRISSRTriticum aestivum LArticle475787800Q1WOS:0010873728000152-s2.0-85175023548#BAŞV!Q2