Canbaz, Mehmet ÇetinŞimşek, Çiğdem SayikliSezgintürk, Mustafa Kemal2022-05-112022-05-1120140003-26701873-4324https://doi.org/10.1016/j.aca.2014.01.041https://hdl.handle.net/20.500.11776/7169We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3. (C) 2014 Elsevier B.V. All rights reserved.en10.1016/j.aca.2014.01.041info:eu-repo/semantics/closedAccessCancer biomarkersHER-3Anti-HER-3Single frequency impedanceBiosensorGrowth-Factor ReceptorBreast-CancerExpressionDispersionAntibodyTherapyFamilyArticle8143138Q1WOS:0003315564000032-s2.0-8489364227824528841Q1