Çaylı, SevilSati, LeylaSeval Çelik, YaseminTuncer, M. AltugYaymaci, BengiBerkman, ZaferDemir, Ramazan2022-05-112022-05-1120100213-39111699-5848https://hdl.handle.net/20.500.11776/8084The preventive and therapeutic effects of Eicosapentaenoic acid (EPA) on diet-induced hyperlipidemia in rabbits have been investigated. Eighteen New Zealand rabbits were randomly divided into three groups of 6 subjects each; experimental group-I (EG-I) was administered a cholesterol rich diet, experimental group-II (EG-II) was treated with EPA (300 mg/kg/d) following a cholesterol-rich diet and the control group (CG) had a standard diet. Blood samples were collected at day 0 and at the 4th and 12th weeks of EG-II to obtain serum levels of total cholesterol (TC), high density lipid-cholesterol (HDL-C), low density lipid-cholesterol (LDL-C) and triglyceride (TG). From each group tissue samples were collected from the carotid artery for immunohistochemistry and electron microscopy. Our results showed that EPA could significantly lower (p<0.001) serum TC, LDL-C, HDLC and TG levels with a reduction of 35%; 55%; 44% and 51%, respectively. Scanning and transmission electron microscopy results revealed that endothelial damage was more prominent in EG-I when compared to EG-II. The ruptured endothelial lining and damaged cellular surface was increased in EG-I when compared to EG-II. Ultrastructural observations showed that after EPA treatment, the degeneration and cellular surface damage on the endothelium were also decreased. These biochemical and ultrastructural results suggest that EPA is a potential drug which significantly lowers the serum lipid profile and partially repairs endothelial dysfunction due to hyperlipidemia.eninfo:eu-repo/semantics/closedAccessEPACholesterolAtherosclerosisEndotheliumUltrastructureRabbitAll-Cis-5,8,11,14,17-Icosapentaenoate Epa-EPolyunsaturated Fatty-AcidsFish-Oil SupplementationBlood-PressureEthyl All-Cis-5,8,11,14,17-IcosapentaenoatePlasma-LipidsDocosahexaenoic AcidLipoproteinsVasculogenesisAngiogenesisArticle252141151Q2WOS:0002728391000022-s2.0-7514919556120017101Q2