Aydin, MuhammetAydin, Elif BurcuSezginturk, Mustafa Kemal2024-10-292024-10-2920240003-26541364-5528https://doi.org/10.1039/d3an01602ahttps://hdl.handle.net/20.500.11776/14380In the current study a simple and highly specific label-free impedimetric neuron specific enolase (NSE) immunosensor based on a copolymer matrix-coated disposable electrode was designed and tested. The copolymer matrix was prepared using a very conductive EDOT monomer and semi-conductive thiophene-bearing epoxy groups (ThEp), and the combination of the two monomers enhanced the conductivity and protein loading capacity of the electrode surface. The P(ThEp-co-EDOT) copolymer matrix was prepared via a drop-casting process and anti-NSE recognition biomolecules were immobilized directly on the epoxy groups of the copolymer. After the coupling of NSE molecules on the P(ThEp-co-EDOT) copolymer matrix-coated electrode surface, the charge transfer resistance (Rct) of the biosensor changed dramatically. These changes in Rct were proportional to the NSE molecule amounts captured by anti-NSE molecules. Under optimized experimental conditions, the increment in the Rct value was proportional to the NSE concentration over a range of 0.01 to 25 pg mL-1 with a detection limit (LOD) of 2.98 x 10-3 pg mL-1. This copolymer-coated electrode provided a lower LOD than the other biosensors. In addition, the suggested electrochemical immuno-platform showed good selectivity, superior reproducibility, long-term stability, and high recovery of NSE in real serum (95.64-102.20%) and saliva (95.28-105.35%) samples. These results showed that the present system had great potential for electrochemical biosensing applications. A P(ThEp-co-EDOT) copolymer matrix was synthesized and utilized as a platform to construct a new NSE biosensor.en10.1039/d3an01602ainfo:eu-repo/semantics/closedAccessNeuron-Specific EnolaseLung-CancerTumor-MarkerBiomarkersBiosensorsDiagnosisLiquidArticle149516321644N/AWOS:0011553732000012-s2.0-8518396299738305417Q1