Kaya, Yasin EmreKaraarslan, NumanYılmaz, İbrahimŞirin, Duygu YaşarYaman, OnurÖzbek, HanefiAteş, Özkan2022-05-112022-05-1120202636-76882636-7688https://doi.org/10.5455/annalsmedres.2019.11.780https://app.trdizin.gov.tr/makale/TXpZeE9URXlNZz09https://hdl.handle.net/20.500.11776/5547AbstractAim: Although antibiotics are generally well-tolerated, they may have cytotoxic effects. The present randomized, double-blind, in vitro study aimed to investigate the effects of tigecycline on cartilage tissue cells and the extracellular matrix.Material and Methods: Cartilage tissues of patients (n = 8) were used for the preparation of primary cell cultures. Tigecycline-treated cell cultures served as the study group. Non-treated cell cultures served as the control group. Analyses were performed at 0, 24, 48, and 72 h in both groups. The results obtained were statistically evaluated. The alpha significance value was determined to be 0.05.Results: Proliferation remained unchanged in the tigecycline-treated cell cultures. The gene expressions of the markers involved in anabolic pathways increased in the tigecycline-treated cell cultures. The results obtained were statistically significant (P < 0.05).Conclusion: Although tigecycline had no toxic effect on the chondrocyte cell cultures and caused no damage to the extracellular matrix, the present study was performed in an in vitro environment.en10.5455/annalsmedres.2019.11.780info:eu-repo/semantics/openAccessArticle27410821087TXpZeE9URXlNZz09