Erboğa, MustafaAktaş, C.Kurt, ÖmerUygur, RamazanÇağlar, VeliTuran, Bünyamin CüneytÖzen, Oğuz Aslan2022-05-112022-05-1120160303-45691439-0272https://doi.org/10.1111/and.12436https://hdl.handle.net/20.500.11776/5138The objective of this study was to examine the effects of thymoquinone (TQ), which has antioxidant properties in the experimental testicular I/R model in rats in terms of its anti-apoptotic, proliferative and biochemical attributes. In our study, 24 male rats were divided into three groups: control group, I/R group and I/R+TQ group. Testicular torsion was created by rotating the left testis 720 degrees in a clockwise direction. The ischaemia period was 4h, and an orchiectomy was performed after 4h of detorsion. Spermatogenesis and the mean seminiferous tubule diameter were significantly decreased in the I/R groups compared to the control group. Furthermore, TQ-treated animals displayed an improved histological appearance in the I/R group. It was also observed that treatment with TQ increased the activity of PCNA, which decreased as a result of I/R, and this treatment also reduced the number of TUNEL-positive cells. The I/R+TQ group showed a decrease in malondialdehyde levels and an increase in the activities of superoxide dismutase, catalase and glutathione peroxidase in comparison with the I/R group. It could be concluded that cytoprotective effects of TQ on the I/R testicles are via reduction of apoptosis, oxidative stress and lipid peroxidation.en10.1111/and.12436info:eu-repo/semantics/closedAccessApoptosisischaemia and reperfusionoxidative stresstestisthymoquinoneLipid-PeroxidationSeminiferous TubulesChronic EthanolRatMelatoninTorsionTestisDamageDeathArticle482222230Q3WOS:0003702243000132-s2.0-8495744105626011095Q2