Aydın, Elif BurcuSezgintürk, Mustafa Kemal2022-05-112022-05-1120190003-2670https://doi.org/10.1016/j.aca.2019.05.049https://hdl.handle.net/20.500.11776/5087A comparative study was made by using two matrices (Linear poly(glycidyl methacrylate) (GMA) polymer (LP(GMA)) and carbon black-polyvinylidene fluoride (PVDF)-LP(GMA) composite CLP(GMA)) as immobilization platforms for anti-IL 1? antibody on the development of impedimetric immunosensor for IL 1? biomarker determination. These materials were spin coated onto the clean ITO electrode surface separately and used for IL 1? immunosensor fabrication. Carbon black is utilized as a conductive material and has been employed over the last few decades for electrochemical biosensors development. The using of CLP(GMA) composite as an interface material shows fast electron transfer, when compared to LP(GMA) modified ITO electrode. This comparative study investigated the efficacy of carbon black for impedimetric biosensing. Anti-IL 1? antibodies were utilized as bioreceptors and bound to epoxy groups of GMA polymer. For electrochemical characterizations, Electrochemical Impedance Spectroscopy, Cyclic Voltammetry and Single Frequency Impedance methods were employed. Furthermore, to follow the antibody attachment on the modified ITO substrates, Fourier Transform Infrared Spectroscopy and Raman spectral techniques were utilized. The morphological characterizations of immunosensor construction stages were carried out by Scanning Electron Microscopy and Atomic Force Microscopy analyses. The sensitive and label-free technique of EIS was used for quantification of IL 1? concentration. Under optimum experimental conditions, the immunosensor had a good linear relationship between impedance values and the IL 1? concentrations ranging from 0.01 to 2 pg/mL and from 0.01 to 3 pg/mL for LP(GMA) and CLP(GMA) modified ITO electrodes, respectively. The results confirmed that CLP(GMA) used in the preparation of the immunosensor illustrated improvements in immunosensor performance, comparing to the LP(GMA) modified immunosensor. Additionally, the immunosensor was successfully applied to evaluate human serum and saliva samples. The obtained results from these samples illustrated the feasibility of the immunosensor for clinical diagnosis in complex biological samples. Also, a simple and low-cost approach was attempted for development of immunosensor with remarkable performance characteristics. © 2019 Elsevier B.V.en10.1016/j.aca.2019.05.049info:eu-repo/semantics/closedAccessDisposable ITO electrodeElectrochemical impedance spectroscopyInterleukin 1?poly(glycidyl methacrylate) polymerAcrylic monomersAntibodiesAtomic force microscopyBody fluidsCarbon blackCoated materialsConductive materialsCyclic voltammetryDiagnosisElectrochemical electrodesFluorine compoundsFourier transform infrared spectroscopyImmunosensorsMatrix algebraPolymer matrix compositesScanning electron microscopyElectrochemical biosensorElectrochemical characterizationsImpedimetric immunosensorsInterleukin-1ITO electrodesMorphological characterizationPerformance characteristicsPoly(glycidyl methacrylate)Electrochemical impedance spectroscopyblack carboncarbon black polyvinylidene fluoride linear poly(glycidyl methacrylate)interleukin 1 antibodyinterleukin 1alphalinear poly(glycidyl methacrylate)polymerpolyvinylidene fluorideunclassified drugIL1A protein, humanimmobilized antibodyindium tin oxideinterleukin 1alphapolyglycidyl methacrylatepolymethacrylic acid derivativepolyvinyl derivativetin derivativeantigen bindingArticleatomic force microscopyblood samplingcomparative studycyclic potentiometryelectrochemical detectionelectron transportFourier transform infrared spectroscopyhumanimpedance spectroscopypriority journalprotein immobilizationRaman spectrometrysaliva analysisscanning electron microscopybloodchemistrydeviceselectrochemical analysiselectrodegenetic proceduresimmunoassayimmunologylimit of detectionproceduressalivasootAntibodies, ImmobilizedBiosensing TechniquesElectrochemical TechniquesElectrodesHumansImmunoassayInterleukin-1alphaLimit of DetectionPolymethacrylic AcidsPolyvinylsSalivaSootTin CompoundsArticle1077129139Q1WOS:0004752998000092-s2.0-8506607520631307701Q1