Arman, K.Saadat, Khandakar A.S.M.Iğcı, Y.Z.Bozgeyik, Esraİkeda, M.A.Çakmak, Ecir AliArslan, A.2022-05-112022-05-1120201065-6995https://doi.org/10.1002/cbin.11434https://hdl.handle.net/20.500.11776/4911Long noncoding RNA (lncRNA) dysregulation is known to be taking part in majority of cancers, including osteosarcoma. In one of our previous studies, we showed that lncRNA MEG3 is being regulated by microRNA-664a (miR-664a) suppresses the migratory potential of osteosarcoma cells (U-2OS). We now report a novel lncRNA, namely, ERICD, which is linked to the transcription factor AT-rich interaction domain 3A (ARID3A) in U-2OS cells. We show that ARID3A binds to ERICD and indirectly interacts with each other via the E2F transcription factor 1 (E2F1). Furthermore, small interfering RNA (siRNA)-mediated knockdown of ERICD inhibited cell migration, formation of colonies, and proliferation in U-2OS cells. Overexpression of ARID3A inhibited cell migration, colony formation, and proliferation, whereas siRNA-mediated knockdown of ARID3A promoted cell migration, colony formation, and proliferation. Our findings indicate that ARID3A and lncRNA ERICD have plausible tumor suppressive and oncogenic functions, respectively, in osteosarcoma. Our data demonstrate the converse interaction between ARID3A and lncRNA ERICD that target DNA-binding proteins and dysregulation of their expression through E2F1 augments osteosarcoma progression. The cell rescue experiment also indicated E2F1 to be involved in the regulation of ARID3A and ERICD. © 2020 International Federation for Cell Biologyen10.1002/cbin.11434info:eu-repo/semantics/closedAccessARID3Acolony formationDNA-binding lncRNAlncRNA ERICDmigrationosteosarcomaDNA binding proteinE2F transcription factor 1long noncoding RNA E2F1 regulated inhibitor of cell deathlong untranslated RNAtranscription factortranscription factor AT rich interaction domain 3Atranscription factor E2Funclassified drugARID3A protein, humanDNA binding proteinE2F1 protein, humanlong untranslated RNAtranscription factortranscription factor E2F1Articlebinding sitecancer growthcell migrationcell proliferationcell viabilitycolony formationcontrolled studydown regulationgene expression regulationgene functiongenetic correlationhumanhuman cellosteosarcomapromoter regionprotein expressionprotein functionprotein RNA bindingregulatory mechanismU2OS cell lineupregulationapoptosisbone tumorcell motioncell proliferationgeneticsmetabolismosteosarcomapathologytumor cell lineApoptosisBone NeoplasmsCell Line, TumorCell MovementCell ProliferationDNA-Binding ProteinsE2F1 Transcription FactorGene Expression Regulation, NeoplasticHumansOsteosarcomaRNA, Long NoncodingTranscription FactorsArticle441122632274Q3WOS:0005578260000012-s2.0-8508925922532749762Q1