Aydın, MuhammetAydın, Elif BurcuSezgintürk, Mustafa Kemal2022-05-112022-05-1120191616-5187https://doi.org/10.1002/mabi.201900109https://hdl.handle.net/20.500.11776/5088In this study, an impedimetric immunosensor based on polymer poly(thiophene)-graft-poly(methacrylamide) polymer (P(Thi-g-MAm)) modified indium tin oxide (ITO) electrode is developed for the detection of the Neuron Specific Enolase (NSE) cancer biomarker. First, the P(Thi-g-MAm) polymer is synthesized and coated on the ITO electrode by using a spin-coating technique. P(Thi-g-MAm) polymer acts as an immobilization platform for immobilization of NSE-specific monoclonal antibodies. Anti-NSE antibodies are utilized as biosensing molecules and they bind to the amino groups of P(Thi-g-Mam) polymer via glutaraldehyde cross-linking. Spin-coating technique is employed for bioelectrode fabrication and this technique provides a thin and uniform film on the ITO electrode surface. This bioelectrode fabrication technique is simple and it generates a suitable platform for large-scale loadings of anti-NSE antibodies. This immunosensor exhibits a wide linear detection range from 0.02 to 4 pg mL?1 and with an ultralow detection limit of 6.1 fg mL?1. It reveals a good long-term stability (after 8 weeks, 78% of its initial activity), an excellent reproducibility (1.29% of relative standard deviation (RSD)), a good repeatability (5.55% of RSD), and a high selectivity. In addition, the developed immunosensor is proposed as a robust diagnostic tool for the clinical detection of NSE and other cancer biomarkers. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen10.1002/mabi.201900109info:eu-repo/semantics/closedAccessdisposable electrodeselectrochemical impedance spectroscopygraft polymerlung cancerneuron-specific enolaseBiomarkersCrosslinkingDiseasesElectrochemical impedance spectroscopyGraft copolymersGrafting (chemical)ImmunosensorsMonoclonal antibodiesNeuronsPlastic coatingsThiopheneTin oxidesDisposable electrodesGlutaraldehyde cross-linkingImpedimetric immunosensorsIndium tin oxide electrodesLinear detection rangesLung CancerNeuron specific enolaseRelative standard deviationsElectrochemical electrodescopolymerindium tin oxidemonoclonal antibodyneuron specific enolasepoly(thiophene) graft poly(methacrylamide) polymertumor markerunclassified drugacrylamide derivativecross linking reagentenolaseglutaraldehydemethacrylamidemonoclonal antibodypolymerpolythiophenethiophene derivativetumor markerArticleatomic force microscopycyclic potentiometryenergy dispersive X ray spectroscopyenzyme immobilizationFourier transform infrared spectroscopyhumanimpedance spectroscopylimit of detectionmeasurementprotein cross linkingproton nuclear magnetic resonanceRaman spectrometryreproducibilityscanning electron microscopyserumsynthesisbloodchemistryelectrochemical analysiselectrodegenetic proceduresimmunoassaypolymerizationproceduresAcrylamidesAntibodies, MonoclonalBiomarkers, TumorBiosensing TechniquesCross-Linking ReagentsElectrochemical TechniquesElectrodesGlutaralHumansImmunoassayLimit of DetectionPhosphopyruvate HydratasePolymerizationPolymersReproducibility of ResultsThiophenesArticle198Q1WOS:0004791035000012-s2.0-8506786720531222894Q1