Daneshvar Royandazagh, Sheida2024-10-292024-10-2920190255-965Xhttps://doi.org/10.15835/nbha47311486https://hdl.handle.net/20.500.11776/12443Lilium candidum L. is one of the most fragrant plant species among Liliaceae. The indigenous and natural populations of L. candidum are significantly affected by fast increasing anthropological population pressure and increased carbon fuel pollution. There is a necessity for development of a micropropagation protocol to serve in effective manner for both commercial multiplication and protection of the plant. The study targeted to develop a strategy for effective in vitro plant propagation system using static liquid culture, seismomorphogenic treatments with mixed liquid culture and semi solid culture medium using single scale explants. The former two techniques failed to regenerate new bulblets effectively whereby the later technique induced 100% regeneration on all treatments. Maximum number of bulblets regenerated on 0.27 ?M Thidiazuron + 1.08 ?M Naphthalene acetic acid. The scale explant could be recultured and regenerated on the mother culture medium for six cycles. The daughter bulblets regenerated on Murashige and Skoog medium containing both 0.18 or 0.27 ?M TDZ + 1.08 ?M NAA were successfully rooted using 1/2 × MS medium containing 2.45 ?M indole butyric acid. Regardless of rooting behavior 100%, acclimatization was noted on all bulblets in the greenhouse. These plants are showing continuous flowering since last four years under hot-summer Mediterranean (CSa type) climate field conditions of Tekirdag province of Thrace region in Turkey. © Not Bot Horti Agrobo, 2019.en10.15835/nbha47311486info:eu-repo/semantics/openAccessIn vitroLilium candidumRootingSingle scale bulbArticle4737347422-s2.0-85071117820Q2