Dinçkaya, ErhanKinik, OzerSezgintürk, Mustafa KemalAltuğ, ÇağrıAkkoca, Aylin2022-05-112022-05-1120110956-56631873-4235https://doi.org/10.1016/j.bios.2011.02.038https://hdl.handle.net/20.500.11776/7116The present work describes the construction and application of a new DNA biosensor for detection of aflatoxin M1. In order to immobilize a thiol-modified single stranded DNA (ss-HSDNA) probe that specifically bound aflatoxin M1, a self-assembled monolayer of cysteamine and gold nanoparticles on the SAM were prepared on gold electrodes, layer-by-layer. The assembly processes of cysteamine, gold nanoparticles, and ss-HSDNA were monitored with the help of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. K-3[Fe(CN)(6)]/K-4[Fe(CN)(6)] solution was used as a redox probe for electrochemical measurements. The biosensor provided a linear response to aflatoxin M1 over the concentration range of 1-14 ng/mL with a standard deviation of +/- 0.36 ng/mL Finally, the biosensor was applied to a series of real milk samples. (C) 2011 Elsevier B.V. All rights reserved.en10.1016/j.bios.2011.02.038info:eu-repo/semantics/closedAccessAflatoxin M1Electrochemical impedance spectroscopyBiosensorGold nanoparticlesSelf-assembled monolayersMycotoxinsElectrochemical ImmunosensorMilk-ProductsM-1ImmunoassayMycotoxinsSamplesFungiElisaM(1)HplcArticle26938063811Q1WOS:00029117820001321420290