Karan, TunayVicil, Sinan2024-10-292024-10-292023979-889113181-1979-889113095-1https://hdl.handle.net/20.500.11776/12666Metabolomics detects, identifies, and quantifies small moleculecontaining metabolites in cells, tissues, and physiological fluids through high-performance yielding technologies. Metabolomics is an integral part of biotechnology, which includes drug activity investigations, enzyme-to-substrate interactions, biomarker identifications, and metabolomic pathway analyses. Metabolite profiling can be achieved cost-effectively in a short time with high precision and specificity through High-Performance Liquid Chromatography (HPLC), one of the analytical methods. Metabolomics research is seen in different fields, including nutrition, toxicology, environmental pollution, drug research and development, and a broad spectrum of biochemical and industrial analyses. Here in this chapter, fundamental basics about HPLC will be given, and the use and importance of HPLC in metabolomics will be highlighted. © 2023 Nova Science Publishers, Inc. All rights reserved.eninfo:eu-repo/semantics/closedAccessChromatographyHPLCIndustryMetabolomicsBook Chapter65822-s2.0-85178611516