Bali, İlhanPolat, Fatin RüştüAziret, MehmetSözen, SelimOruc, CemCoskunkan, UfukKoç, AhmetBilir, BülentEmir, Seyfi2022-05-112022-05-1120180020-8868https://doi.org/10.9738/INTSURG-D-15-00301.1https://hdl.handle.net/20.500.11776/5321Objective: Anastomotic leaks are one of the chief complications after gastrointestinal surgery. The aim of this study was to evaluate whether Nigella sativa administration protects against ischemia/reperfusion injury on healing of colonic anastomosis in rats. Method: Thirty male Wistar albino rats, weighing between 200 and 240 g, were used in the study. They were randomly divided into three groups (n = 10 for each group): Anastomosis (group 1), anastomosis and ischemia/reperfusion injury (group 2), and treatment group of anastomosis, ischemia/reperfusion injury, and Nigella sativa (group 3). After 7 days, serum, plasma, and colonic tissue were obtained and then all rats were sacrificed. Tissue and serum level of total oxidant status, total antioxidant status, total thiol levels, hydroxyproline, interleukin-6, and TNF-alpha were determined and specimens were histopathologically evaluated. Results: In the Nigella sativa treated rats, serum hydroxyproline levels were significantly higher, while tissue levels were significantly lower than those seen in group 1 and group 2 (P = 0.007, P = 0.01, respectively). In the Nigella sativa group, the serum levels of TNF-alpha were significantly lower than those seen in group 1 and 2 (P = 0.001). Also, in group 3, the tissue IL-6 level was significantly higher than that seen in group 1 and group 2 (P = 0.009). The histopathologic analysis showed less edema and inflammatory cell infiltration in the Nigella sativa treated group, as well as a statistically significant difference according to the Chiu classification (P < 0.05). Conclusion: The results of this study indicate that Nigella sativa has a protective and therapeutic effect against ischemia/reperfusion injury on the healing of colonic anastomosis in rats.en10.9738/INTSURG-D-15-00301.1info:eu-repo/semantics/openAccessAnastomosisAnastomotic leaksNigella sativaIschemia/reperfusion injuryIschemia-Reperfusion InjuryMucosal LesionTissue-DamageOilLeakageThymoquinoneModulationPreventionArticle10345385139148Q4WOS:0004746073000042-s2.0-85069832347Q4