Impedimetric Detection of Calreticulin by a Disposable Immunosensor Modified with a Single-Walled Carbon Nanotube-Conducting Polymer Nanocomposite

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dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-04-20T08:01:18Z
dc.date.available2023-04-20T08:01:18Z
dc.date.issued2022
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractA label-free impedimetric immunosensing system was constructed for ultrasensitive determination of the calreticulin (CALR) biological marker in human serum samples utilizing an electrochemical impedance spectroscopy analysis technique for the first time. The new biosensor fabrication procedure consisted of electro-deposition of single-walled carbon nanotubes (SWCNTs) incorporating polymerization of an oxiran-2-yl methyl 3-(1H-pyrrol-1-yl) propanoate monomer (Pepx) onto a low-cost and disposable indium tin oxide (ITO) electrode. The SWCNTs-PPepx nanocomposite layer was prepared onto the ITO after the one-step fabrication procedure. The fabrication procedure of the immunosensor and the characteristic biomolecular interactions between the anti-CALR and CALR were characterized by electrochemical analysis and morphological monitoring techniques. Under optimum conditions, the proposed biosensor was responsive to CALR concentrations over the detection ranges of 0.015-60 pg/mL linearly, and it had a very low detection limit (4.6 fg/mL) and a favorable sensitivity (0.43 k omega pg(-1) mL cm(-2)). The reliability of the biosensor system in clinical analysis was investigated by successful quantification of CALR levels in human serum. Moreover, the repeatability and reproducibility results of the biosensor were evaluated by using Dixon, Grubbs, T-test, and F-tests. Consequently, the proposed biosensor was a promising method for scientific, rapid, and successful analysis of CALR in human serum samples.
dc.description.sponsorshipScientific and Technological Research Council of Canakkale Onsekiz Mart University [FHD-2021-3533]
dc.description.sponsorshipThe authors gratefully acknowledge support from project FHD-2021-3533 of the Scientific and Technological Research Council of Canakkale Onsekiz Mart University. We thank Assoc. Prof. Pinar Ilgin for her support in the FTIR analysis.
dc.identifier.doi10.1021/acsbiomaterials.2c00499
dc.identifier.endpage3784
dc.identifier.issn2373-9878
dc.identifier.issue9en_US
dc.identifier.pmid35920068
dc.identifier.startpage3773
dc.identifier.urihttps://doi.org/10.1021/acsbiomaterials.2c00499
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10858
dc.identifier.volume8
dc.identifier.wosWOS:000855274100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Elif Burcu
dc.institutionauthorAydın, Muhammet
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Biomaterials Science & Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBreast Cancer
dc.subjectCalreticulin
dc.subjectDisposable Immunosensor
dc.subjectImpedimetric Detection
dc.subjectElectrochemical Immunosensors
dc.subjectBreast
dc.subjectBiosensor
dc.subjectSerum
dc.subjectImmobilization
dc.subjectNanomaterials
dc.subjectStrategies
dc.subjectAptamer
dc.titleImpedimetric Detection of Calreticulin by a Disposable Immunosensor Modified with a Single-Walled Carbon Nanotube-Conducting Polymer Nanocomposite
dc.typeArticle

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