Transuterine Fetal Tracheal Occlusion Model in Mice

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dc.authorid0000-0001-7776-9684
dc.authorid0000-0001-9132-9271
dc.authorwosidAydin, Emrah/G-4000-2017
dc.authorwosidOria, Marc/K-9599-2019
dc.contributor.authorAydın, Emrah
dc.contributor.authorJoshi, Rashika
dc.contributor.authorOria, Marc
dc.contributor.authorLim, Foong-Yen
dc.contributor.authorVarisco, Brian Michael
dc.contributor.authorPeiro, Jose Luis
dc.date.accessioned2022-05-11T14:04:53Z
dc.date.available2022-05-11T14:04:53Z
dc.date.issued2021
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Çocuk Cerrahisi Ana Bilim Dalı
dc.description.abstractFetal tracheal occlusion (TO), an established treatment modality, promotes fetal lung growth and survival in severe congenital diaphragmatic hernia (CDH). Following TO, retention of the secreted epithelial fluid increases luminal pressure and induces lung growth. Various animal models have been defined to understand the pathophysiology of CDH and TO. All have their own advantages and disadvantages such as the difficulty of the technique, the size of the animal, cost, high mortality rates, and the availability of genetic tools. Herein, a novel transuterine model of murine fetal TO is described. Pregnant mice were anesthetized, and the uterus exposed via a midline laparotomy. The trachea of selected fetuses were ligated with a single transuterine suture placed behind the trachea, one carotid artery, and one jugular vein. The dam was closed and allowed to recover. Fetuses were collected just before parturition. Lung to body weight ratio in TO fetuses was higher than that in control fetuses. This model provides researchers with a new tool to study the impact of both TO and increased luminal pressure on lung development.
dc.identifier.doi10.3791/61772
dc.identifier.issn1940-087X
dc.identifier.issue168en_US
dc.identifier.pmid33616107
dc.identifier.urihttps://doi.org/10.3791/61772
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4827
dc.identifier.wosWOS:000646178300021
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorAydın, Emrah
dc.language.isoen
dc.publisherJournal Of Visualized Experiments
dc.relation.ispartofJove-Journal Of Visualized Experiments
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleTransuterine Fetal Tracheal Occlusion Model in Mice
dc.typeArticle

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