A Biosensor Based on Zucchini (Cucurbita Pepo L.) Homogenate as a Biorecognition Layer for Ascorbic Acid Determination

dc.authorwosidMENGÜLLÜOĞLU, UMUT/AAG-7084-2021
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidDİNÇKAYA, ERHAN/AAG-7096-2021
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.contributor.authorKoca, Halit Buğra
dc.contributor.authorÖzben, Yavuz Selim
dc.contributor.authorDinçkaya, Erhan
dc.date.accessioned2022-05-11T14:29:41Z
dc.date.available2022-05-11T14:29:41Z
dc.date.issued2010
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractAn amperometric biosensor based on zucchini (Cucurbita pepo) tissue homogenate is presented. The zucchini tissue homogenate was crosslinked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. The zucchini tissue contained the enzyme ascorbate oxidase and this enzyme catalyzed the oxidation of ascorbic acid in the presence of dissolved oxygen. The principle of the measurements was based on the determination of the decrease in the dissolved oxygen level. Determinations were carried out by standard curves, which were obtained by the measurement of the decrease in the oxygen level related to ascorbic acid concentration. Optimization and characterization studies of the biosensor were carried out in detail. First of all, the amounts of zucchini tissue homogenate, gelatin, and glutaraldehyde percentage were optimized. Experimental parameters such as buffer system, pH, buffer concentration, and temperature were also optimized carefully. Thermal stability, storage stability, and repeatability of the biosensor were investigated. A linear response was observed from 5x10(-6) M to 1.2x10(-3) M ascorbic acid. Finally, the results of some plant and drug samples analyzed with the presented biosensor compared with the spectrophotometric method (Tillman reagent) used as a reference.
dc.identifier.doi10.3109/10731191003785588
dc.identifier.endpage221
dc.identifier.issn1073-1199
dc.identifier.issn1532-4184
dc.identifier.issue4en_US
dc.identifier.pmid20384439
dc.identifier.startpage215
dc.identifier.urihttps://doi.org/10.3109/10731191003785588
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7099
dc.identifier.volume38
dc.identifier.wosWOS:000281151900008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kema
dc.language.isoen
dc.publisherInforma Healthcare
dc.relation.ispartofArtificial Cells Blood Substitutes and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectascorbic acid
dc.subjectbiosensor
dc.subjectascorbate oxidase
dc.subjecttissue based biosensor
dc.subjectVitamin C
dc.subjectVitamin-C
dc.subjectModified Electrodes
dc.subjectElectrocatalytic Determination
dc.subjectOxidase
dc.subjectEnzyme
dc.subjectFilm
dc.titleA Biosensor Based on Zucchini (Cucurbita Pepo L.) Homogenate as a Biorecognition Layer for Ascorbic Acid Determination
dc.typeArticle

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