Identification and Assemblage Types of Giardia duodenalis from Patients in Thrace, Turkey

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dc.authoridAkyildiz, Gurkan/0000-0002-8610-5174
dc.contributor.authorKucuk, Sadiye Kaplan
dc.contributor.authorAkyildiz, Gurkan
dc.contributor.authorBircan, Rifat
dc.contributor.authorYilmazer, Nadim
dc.contributor.authorGargili, Aysen
dc.contributor.authorKar, Sirri
dc.date.accessioned2024-10-29T17:59:44Z
dc.date.available2024-10-29T17:59:44Z
dc.date.issued2019
dc.departmentTekirdağ Namık Kemal Üniversitesi
dc.description.abstractObjective: Giardiasis is a common disease, and clinical forms can vary based on the assemblage types of the parasite. Detailed information on the subgenotypes may indicate the transmission routes and enlighten the gaps in the epidemiology of the disease. This study aims to reveal the occurrence of giardiasis in Thrace, Turkey, and assemblage types of Giardia duodenalis. Materials and Methods: In total, 573 stool samples taken from the individuals applied to Tekirdag Central State Hospital in 2009, were examined by wet-mount and zinc sulfate floatation methods. Giardia-positive 26 samples and 64 samples taken from the individuals with gastrointestinal complaints were analyzed by nested PCR-RFLP to differentiate the assemblage types. Sequence analysis was employed for confirmation of assemblage types and subgenotypes. Results: Giardia spp. cysts were detected in 3.66% and 4.54% of the samples with wet-mount and zinc sulphate floatation techniques respectively. A total of 27 samples were found positive by nested PCR-RFLP out of 90 samples. Fifteen samples were determined as assemblage A, 2 and 10 samples as B and B/E mix respectively. Sequence analysis showed that the latter assemblage (B/E mix) as A3-B3 mix. Conclusion: Fast identification techniques, namely zinc sulphate flotation can be used for screening stool samples in order to determine Giardia cysts with considerably high sensitivity and specificity. Based on this method, the occurrence rate of giardiasis was found as 4.54% in the studied group. DNA sequencing is necessary to distinguish assemblages and confirm the results of PCR-RFLP.
dc.identifier.doi10.5152/idcm.2019.19001
dc.identifier.endpage13
dc.identifier.issn2667-646X
dc.identifier.issue1en_US
dc.identifier.startpage6
dc.identifier.urihttps://doi.org/10.5152/idcm.2019.19001
dc.identifier.urihttps://hdl.handle.net/20.500.11776/14825
dc.identifier.volume1
dc.identifier.wosWOS:001189478100003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherDoc Design Informatics Co Ltd
dc.relation.ispartofInfectious Diseases and Clinical Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGiardia duodenalis
dc.subjectassemblage
dc.subjectsubgenotype
dc.subjecthuman
dc.subjectTurkey
dc.titleIdentification and Assemblage Types of Giardia duodenalis from Patients in Thrace, Turkey
dc.typeArticle

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