Yazar "Tuna, M." seçeneğine göre listele
Listeleniyor 1 - 5 / 5
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Cytogenetic and molecular responses of ammonium sulphate application for tolerance to extreme temperatures in Vicia faba L.(Allerton Press Inc, 2015) Öney, S.; Tabur, S.; Tuna, M.Effects of ammonium sulphate [(NH4)(2)SO4] on mitosis, cell cycle and chromosomes in Vicia faba L. seeds exposed to extreme temperatures were investigated using flowcytometric and cytogenetic analysis. Seeds germinated at high and low temperatures showed a significant decrease in mitotic index as compared to those of optimum temperature conditions. Application of 50 and 1000 mu M (NH4)(2)SO4 were successful in alleviating the negative effects of low and high temperature on mitotic activity, respectively. 50 mu M (NH4)(2)SO4 showed the most positive effect on cell cycle at the extreme temperatures. This concentration increased the cell division removing or decreasing the negative effects of temperature stress. Namely, the highest G2/M and S phase percentages under stress conditions were obtained with application of 50 mu M (NH4)(2)SO4. Chromosomal aberrations were not observed in cells of seeds germinated in distilled water and also at any temperatures. However, the frequency of chromosomal aberrations increased significantly by increasing (NH4)(2)SO4 concentration. The highest aberration frequency in all temperature degree tested was found at 1000 mu M (NH4)(2)SO4 concentration.Öğe Determination of nuclear dna content and ploidy of some bromus l. Germplasm by flow cytometery(Pakistan Botanical Society, 2020) Nizam, İlker; Gülcü, R.; Tuna, G.S.; Tuna, M.The objective of this study was to determine nuclear DNA content and ploidy of 48 Bromus L. gene bank accessions obtained from USDA by flow cytometry. Based on the results of the flow cytometric analysis, the mean 2C nuclear DNA content of Bromus accessions used in the study varied between 11.43 pg with 26.62 pg. The nuclear DNA content differences among Bromus L. accessions were statistically significant at P<0.01 level. The accessions were easily separated into three distinct groups according to their DNA contents. The mean 2C nuclear DNA content of the groups were 11.82, 22.43 and 26.17 pg (min. and max. values of group were between 11.43–12.65 pg, 21.45–22.77 pg, 25.48–26.62 pg, respectively). Nuclear DNA content of the accessions were correlated with their ploidy levels by counting chromosomes of the one plant from each group with classical staining methods. In conclusion, majority of the Bromus accessions (33 out of 48) analysed in the study were tetraploid with 2n=4x=28 chromosomes. Ten and five of the remaining accessions were octaploid (2n=8x=56) and (2n=10x=70) decaploids, respectively. No decaploid B. inermis plant reported until today. However, decaploids were reported for B. riparius and B. biebersteini species. Therefore, decaploid plants determined in this study should be considered as either B. riparius or B. biebersteini. © 2020, Pakistan Botanical Society. All rights reserved.Öğe Parlak Brom (Bromus catharticus Vahl.) Hatlarının Ot Verimi ve Bazı Morfolojik Özelliklerinin Belirlenmesi(Namik Kemal University - Agricultural Faculty, 2021) Bato, E.; Nizam, İlker; Tuna, M.The aim of this research was to determine the forage yield performance and some morphological characteristics of nine different prairie grass (Bromus catharticus Vahl.) line in the conditions of Tekirdag and Edirne. The experiments was carried out in randomized complete block design with three replications at the experimental areas of Field Crops Department of Agricultural Faculty of Tekirdag Namik Kemal University and Directorate of Trakya Agricultural Research Institute in 2015-2016 years. Morphological characters such as main stem lenght, main stem thickness, internode lenght, main stem node number, flag leaf width and lenght with hay and fresh yield were investigated in the study. Differences among lenght of main stem, thickness of main stem, internode lenght, node number of main stem, lenght of flag leaf, width of flag leaf, fresh and hay yields of the prairie grass lines were found to be statistically very significant. The lenght of main stem varied between 72.57-99.05 cm in Tekirdag location and 94.36-111.73 cm in Edirne location. The thickness of the main stem varied between 1.58-2.33 mm in Tekirdag location and 2.57-4.52 mm in Edirne location. Internode lenght varied between 9.95-13.26 cm in Tekirdag location and 14.09-22.30 cm in Edirne location. The node number of main stem varied between 3.93-4.67 in Tekirdag location and 3.47-4.60 in Edirne location. Lenght of flag leaf varied between 23.76-26.40 cm in Tekirdag location and 17.46-26.40 cm in Edirne location. Width of flag leaf varied between 4.93-8.90 mm in Tekirdag location and 2.84-4.85 mm in Edirne location. Fresh yield of linesvaried between 2.208,57-3.521,27 kg da-1 in Tekirdag 2.567,73-4.032,06 kg da-1 in Edirne conditions. Hay yield of lines varied between 638.89-956.07 kg da-1 in the conditions of Tekirdag, and 739.24-1.075,44 kg da-1 in the conditions of Edirne. The highest fresh and hay yield was determined from 197848 line in Edirne location and from 217583 and 197848 lines in the location of Tekirdag where the research was conducted. Based on the results of this study, prairie grass lines 197848 and 217583 can be suggested for cultivation in the Thrace part of Turkey. © Bu calisma Tekirdag Namik Kemal Universitesi tarafindan Creative Commons Lisansi.Öğe SOME CYTOLOGICAL and AGRONOMIC CHARACTERISTICS of PERENNIAL RYEGRASS (LOLIUMPERENNE L.) ACCESSIONS COLLECTED from NATURAL FLORA(Parlar Scientific Publications, 2020) Nizam, İlker; İnal, C.; Tuna, M.The objective of this study was to determine cytogenetic and agronomic characteristics of 37 peren-nial ryegrass (Lolium perenne L.) accessions, collected from natural flora in Turkey, in 2010. Nuclear DNA content of the accessions was measured by flow cytometer, using propidium iodide as fluoro-chrome and the information was used to determine the ploidy of the accessions. The field trial was carried out in the experimental fields of the Department of Field Crops, Faculty of Agriculture, Tekirdag Namik Kemal University and five agronomic characters such as plant height, stem diameter, tiller number, fresh yield per plant, hay yield per plant were investigated for two years between 2012 and 2014. In addition, the correlation between DNA content and morphological characters was evaluated. Based on the results of the nuclear DNA content analysis, 2C nuclear DNA content of accessions vary between 5.354 and 6.004 pg. Nuclear DNA content of the accessions were correlated by counting the mitotic chromosomes of the accessions, by using a classical squash technique. It was found out that all the accessions used in chromosome counting had 2n=14 chromosomes, indicating that all the accessions used in the study were diploid. According to the results obtained from field experiments, mean plant height, stem diameter, tiller number, fresh yield per plant and dry yield per plant of perennial ryegrass accessions varied between 57.05-90.80 cm, 1.31-2.00 mm, 60.10-397.50 number, 163.55-730.00 g, 49.65-164.00 g, respectively. Wide variation was observed among accessions used in the study for both, cytological and agronomic charac-ters. According to the Pearson correlation analysis, hay yield per plant was positively and significantly correlated with plant height and tiller number. There was a negative correlation between DNA content and all characters, except for hay yield per plant. © 2020 Parlar Scientific Publications. All rights reserved.Öğe Taxonomic classification and identification of fine leaved fescues collected from Eastern Anatolia of Turkey using flow cytometer(International Society for Horticultural Science, 2020) Savaş Tuna, Gülsemin; Yılmaz, E.C.; Ulutaş, N.; Şahin, B.; Nizam, İlker; Yücel, G.; Tuna, M.Fescues are a very complex group of grasses of the Poaceae family. They have a wide adaptation as they are distributed all over the world. The close morphological resemblance of species (Festuca spp.) makes their identification and classification difficult. Therefore, it is not clear how many true species are included in the genus Festuca, but the estimated range is beween 400 and 500 species. It is well-known that nuclear DNA content among species varies considerably, and it accompanies the evolution of species and species groups. This is mainly because of chromosomal variations, but similar differences also exist among closely related species with the same chromosome number. Also, natural hybridization due to open pollination presents difficulties in distinguishing closely related taxa using morphological analysis. The objective of this study was to determine nuclear DNA content and ploidy level of over 130 populations of fescues collected from the natural flora of Eastern Anatolia by flow cytometer using PI as Fluorochrome. Based on the results of nuclear DNA content analysis, 2C nuclear DNA content of populations varied between 4.63 and 15.03 pg. These results indicate that fescue genetic materials collected from nature have a mixture of different species and ploidy levels. Therefore, prior characterization is a necessity before including them in breeding programmes. The results also indicated that flow cytometry provides easy, fast, relatively economic and reliable method to screen newly collected populations of plant genetic resources. © 2020 International Society for Horticultural Science. All rights reserved.
