Yazar "Teke, Mustafa" seçeneğine göre listele

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  • Küçük Resim
    Öğe
    A Novel Biosensing System Using Biological Receptor for Analysis of Vascular Endothelial Growth Factor
    (Springer, 2014) Teke, Mustafa; Sayıklı, Çiğdem; Canbaz, Çetin; Sezgintürk, Mustafa Kemal
    An immunosensor with rapid and ultrasensitive response for vascular endothelial growth factor (VEGF) has been built up with 4-aminothiophenol (4-ATP) onto the gold surfaces. Quantitative analysis of VEGF was performed by recording the impedance changing of the gold electrode surface by binding of VEGF. The human vascular endothelial growth factor receptor 1 (VEGF-R1, Flt-1) was used as a biorecognition element for the first time in the literature. VEGF-R1 was covalently immobilized via 4-ATP self-assembled monolayer formed on gold thin film covered surface. Construction of the biosensor was carefully characterised by the techniques such as electrochemistry and electrochemical impedance spectroscopy. In order to characterize impedance data, Kramers-Kronig transform was performed on the experimental impedance data. The limit of detection of the immunosensor for qualitative detection was 100 pg/mL while the LOD for quantitative detection could down to 100 pg/mL by using the VEGF-R1 based biosensor. Finally, artificial serum samples spiked with VEGF was analyzed by the proposed immunosensor to investigate useful of the biosensor for early biomarker diagnosis.
  • Küçük Resim
    Öğe
    An ITO Based Disposable Biosensor for Ultrasensitive Analysis of Retinol Binding Protein
    (Wiley-V C H Verlag Gmbh, 2014) Sayıklı Şimşek, Çiğdem; Teke, Mustafa; Sezgintürk, Mustafa Kemal
    A highly sensitive electrochemical biosensor based on anti-RBP biorecognition capable to analyze concentrations of retinol binding protein (RBP) was developed. The construction of the biosensor interfaces was carefully characterized by techniques such as electrochemistry, EIS, and scanning electron microscopy. In order to characterize impedance data, Kramers-Kronig Transform was performed on the experimental impedance data. Besides, for an immunosensor system the Single Frequency Impedance technique was firstly used for the characterization of the interaction between RBP and anti-RBP. Finally, artificial serum samples spiked with RBP were analyzed by the proposed ITO based immunosensor to investigate the usefulness of the biosensor for early biomarker diagnosis.