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Öğe Foliar Resistance to a Natural Population of Plasmopara viticola in Some Grapevine Varieties Grown in Turkiye(Wiley, 2024) Dogu, Duygu Mermer; Ozer, NurayIt is critical to use a population of the pathogen that contains different strains to determine the resistance of grapevine varieties to the downy mildew pathogen Plasmopara viticola in a specific region. This study tested six local grape varieties (Cavus, Kalecik Karasi, Muskule, Papaz Karasi, Sultana and Yapincak), eight Turkish registered varieties (Baris, Bozbey, Guzgulu, Ozer Karasi, Recel uzumu, Tekirdag Cekirdeksizi, Trakya & Idot;lkeren and Yalova Incisi) and seven varieties of foreign origin (Cabernet Sauvignon, Cardinal, Chardonnay, Cinsault, Isabella, Semillon and Gamay) using detached leaf and leaf disc assays with pathogen populations collected from vineyards. There was a significant correlation for disease severity between the two assay methods. No sporulation was observed on the varieties Cavus and Isabella in both assay methods. The variety Yapincak had very low disease severity (2.00%) in the leaf disc assay, and the pathogen did not sporulate on this variety in the detached leaf assay. Cluster analysis was performed to determine the resistance level of the varieties against the pathogen, as different disease severities were observed in some varieties depending on the test method used, and the 21 varieties were classified into four clusters. Baris, Bozbey, Cardinal, Cavus, Isabella, Semillon and Yapincak in Cluster I exhibited the lowest disease severity mean (2.88%), while those in Cluster 3, including Cabernet Sauvignon, Guzgulu, Papaz Karasi and Yalova Incisi, had the highest disease severity mean (56.75%). These varieties in Clusters 1 and 3 were considered highly resistant and highly susceptible, respectively. Leaf hair density, as reported in official variety descriptions, did not associate with reduced disease severity in the two assays. This study assessed the response of grapevine varieties to the P. viticola population in the region for the first time. The highly resistant and resistant varieties identified will provide new material for breeders and contribute to the improvement of organic viticulture in the area.Öğe Identification of seed-borne fungi in summer (Cucurbita pepo) and winter (Cucurbita moschata) pumpkins of Turkey(Springer, 2023) Demir, Edanur; Ozer, Nuray; Bayraktar, HarunThis study used seeds from five different summer pumpkin (Cucurbita pepo L.) cultivars (caglayan, Mert Bey, Sena Hanim, TG22, and TG38) and seeds collected from fruits in ten different provinces in Turkey where winter pumpkins (Cucurbita moschata Duch.) were cultivated. The seed coat and embryo were cultured and the seed-borne fungi were identified. Allophoma labilis and Didymella americana were detected in seeds (seed coat and embryo) of the cvs. caglayan and Mert Bey only, respectively. The seeds of both summer and winter pumpkins were contaminated with Alternaria alternata, Fusarium equiseti and Fusarium proliferatum, though their incidence varied depending on cultivar, province and seed parts. All species caused root and crown rot in seedlings in pathogenicity tests. Disease severities were caused by A. labilis, A. alternata, D. americana, F. equiseti and F. proliferatum at rates of 37.14-49.64%, 31.78%-42.50%, 31.29%, 14.17-50.00% and 25.14-51.07%, respectively. Colonial growth on different media, morphological and molecular characterization of the most pathogenic isolates from each species were defined. This is the first report in Turkey of A. labilis and D. americana in seeds of C. pepo, and Alternaria alternata, F. equiseti and F. proliferatum in seeds of both C. pepo and C. moschata, as well as the first pathogenicity record for these species by seed inoculation.Öğe Molecular and pathogenic characterization of Fusarium oxysporum f. sp. pisi isolates obtained from Turkey(Springer, 2024) Gultekin, Mehmet Akif; Ozer, Nuray; Ozer, GokselFusarium oxysporum f. sp. pisi (Fop) is a highly destructive root rot pathogen for the pea (Pisum sativum L.) plant. In this study, the presence of F. oxysporum isolates was detected during the 2020-2021 growing season in plants showing symptoms of root rot in provinces of Turkey (Bursa, Afyonkarahisar, & Idot;zmir and Ayd & imath;n) important for pea production. The pathogen was identified by its colony growth on various media, morphological characteristics and, molecular characterization based on DNA sequencing and SCoT markers. The pathogenicity of 183 isolates obtained from the roots of disease symptomatic plants was tested after artificial inoculation of pruned roots of seedlings by dipping in a conidia suspension of the isolates. Disease severity was scored and the length of brown lesions on the stem, extending from the base, was measured for each isolate. The isolates showed very large variation (0%-80%) for disease severity. A total of 50 isolates showing high stem-lesion lengths within different disease severity ranges were selected and evaluated for their effect on plant growth parameters such as plant height, number of seeds per pod and number of pods per plant. There were significant positive correlations between the reduction (%) in plant height and lesion length, and disease severity. These isolates can potentially cause yield losses in Turkey's pea cultivation based on the measured stem lesions which could be an indication of the pathogenicity of different Fop isolates. New information about pathogenic variability in Fop isolates from Turkey will be valuable for detecting races of this pathogen and resistance of pea genotypes.Öğe Molecular and pathogenic characterization of Fusarium oxysporum f. sp. pisi isolates obtained from Turkey (vol 171, pg 37, 2024)(Springer, 2025) Gultekin, Mehmet Akif; Ozer, Nuray; Ozer, Goksel[No abstract available]
