Yazar "Orak, Hakime Hülya" seçeneğine göre listele
Listeleniyor 1 - 20 / 36
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A new pyran derivative analog from the whole plant of Cirsium italicum(Taylor & Francis Ltd, 2021) Çalışkan, Hilmican; Özer, Merve; Şabudak, Temine; Orak, Hakime HülyaThe aim of the present article was to analyze the phytochemical composition of EtOAc extract ofCirsium italicum(Savi) DC. and to determine the antioxidant activity of a new compound1. The EtOAc extract ofC. italicumwas purified by different chromatographic methods. Compound1was isolated from the whole plant ofC. italicum. Its structure was established on the basis of UV, IR, 1 D, 2 D NMR, and HRESI-MS methods and antioxidant activities of this compound were investigated using TEAC, FRAP, and CUPRAC assays. According to the ABTS assays, the TEAC capacity of compound 1 was determined as 0.186 mmol TE/g. [GRAPHICS] .Öğe Antibacterial and antifungal activity of pomegranate (Punica granatum L.cv.) peel(2011) Orak, Hakime Hülya; Demirci, Ahmet Şükrü; Gümüş, TuncayPunica granatum L, cv. Hicaznar and three different genotypes, which are named genotype 19-121, 17-67and 19-66 which have been obtained at Bati{dotless} Akdeniz Agricultural Research Institute (BATEM) in Antalya, have been studied for determination of their antibacterial and antifungal activities. The antibacterial activity of peel extracts was tested against three bacteria strains, which were named Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922) and Salmonella Enteritidis (ATCC 13076). The antifungal activity was tested against two fungal strains, which were named Aspergillus parasiticus NRRL 2999 and Aspergillus parasiticus NRRL 465. All extracts possessed remarkable antibacterial and antifungal activities against all tested bacterial and fungal strains. Among the extracts genotype 19-66 peel extracts had lowest antimicrobial activity, although its peel had highest total phenolic content. Also its acidity was determined to be remarkably lowest as compared other Punica granatum L peels. This result may explain that antibacterial effect of phenolic compounds could be increasing with presence of organic acids.Öğe Antioxidant Activity and Total Phenolic Contents of Different Alfalfa (Medicago sativa L.) Varieties Grown in Turkey(Centenary University, 2021) Orak, Hakime Hülya; Karamac, Magdalena; Orak, Adnan; Tenikecier, Hazım Serkan; Amarowicz, RyszardFifteen alfalfa varieties cultivated in Turkey were researched to compare the antioxidant potential of their seeds and find differences between the varieties. The total phenolic (TP) content, DPPH•, and ABTS•+ scavenging activities, and ferric-reducing antioxidant power (FRAP) of alfalfa seed methanol extracts were determined. TP contents of extracts were found between 37.03 and 54.04 mg GAE/g. ABTS assay results ranged between 0.100 and 0.158 mmol Trolox/g extract, and FRAP changed from 389.90 to 791.02 µmol Fe2+/g extract. The results demonstrated that the differences between the alfalfa varieties were significant (p < 0.01) in terms of analyzed characters. © 2021, Centenary University. All rights reserved.Öğe Antioxidant Activity of Extracts of Soursop (Annona muricata L.) Leaves, Fruit Pulps, Peels, and Seeds(Inst Animal Reproduction & Food Research Polish Acad Sciences Olsztyn, 2019) Orak, Hakime Hülya; Bahrisefit, İlayda Şevik; Şabudak, TemineThe total phenolic content (TPC), total flavonoid content (TFC), and the antioxidant activity of soursop (Annona muricata L.) leaf, fruit pulp, seed, and peel extracts obtained using successive extraction with hexane (Hxn), dichloromethane (DCM), ethyl acetate (EtOAc), and methanol (MeOH) were determined. The Hxn soursop seed extract was analysed by GC-MS. The highest TPC was determined in MeOH extracts. MeOH and EtOAc extracts were rich sources of flavonoids. Generally, soursop leaf and fruit pulp extracts had the highest and the lowest both TPC and TFC, respectively. Fatty acids were dominant in the Hxn seed extract. Among antioxidants, terpenoids ((E)-nerolidolas as dominant) and phytosterols ((3-beta)-stigmast-5-en-3-ol with high content) were identified. The soursop seed, followed by leaf and peel extracts (MeOH and EtOAc) had the highest DPPH center dot scavenging activity, TEAC, FRAP, and CUPRAC. Antioxidant activity of peel extracts (MeOH and EtOAc) was particularly high in beta-carotene-linoleic acid emulsion system. Strong correlations were found between TPC, TFC, TEAC, FRAP, and results of DPPH assay. In conclusion, soursop leaves and fruit seeds and peels, which are cheap, waste plant material, could be considered as a source of phenolic antioxidants with a high antioxidant activity.Öğe Antioxidant activity of five new phenolic compounds from Cirsium creticum subsp. creticum(Elsevier Science Bv, 2019) Şabudak, Temine; Özer, Merve; Orak, Hakime Hülya; Çalışkan, HilmicanIn this study, five new alkyl catechol derivatives, 1, 2, 3, 4 and 5 were isolated for the first time from Cirsium creticum subsp. creticum L. and their structures were determined by using spectroscopic techniques, including mass spectrometry, 1D and 2D NMR. All of the isolated compounds were primarily evaluated for antioxidant activity against BHA and tocopherol. All compounds which were isolated from C. creticum subsp. creticum exhibited antioxidant activity, however it was noticed that the presence of a pentose sugar increased the antioxidant activity.Öğe ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS OF RED BEAN (Phaseolus vulgaris L.)(Scibulcom Ltd, 2015) Orak, Hakime Hülya; Karamac, Magdalena; Amarowicz, RyszardAntioxidant potential of seeds of commonly cultivated two read bean cultivars, namely Onceler and Mecidiye, was evaluated. The crude extracts of phenolic compounds were obtained using by 80% (v/v) methanol and acetone. The antioxidant potential of the seeds was tested using TEAC, FRAP, and DPPH method. The presence of phenolic acids and flavonoids was confirmed using HPLC method. The results of the present study showed red kidney bean, cultivated in Turkey, to be potentially valuable legume crops with high antioxidant potential.Öğe Antioxidant activity of phenolic compounds of red bean (Phaseolus vulgaris L.)(Scibulcom Ltd., 2015) Orak, Hakime Hülya; Karamac, Magdalena; Amarowicz, RyszardAntioxidant potential of seeds of commonly cultivated two read bean cultivars, namely Onceler and Mecidiye, was evaluated. The crude extracts of phenolic compounds were obtained using by 80% (v/v) methanol and acetone. The antioxidant potential of the seeds was tested using TEAC, FRAP, and DPPH method. The presence of phenolic acids and flavonoids was confirmed using HPLC method. The results of the present study showed red kidney bean, cultivated in Turkey, to be potentially valuable legume crops with high antioxidant potential. © 2015, Scibulcom Ltd. All rights reserved.Öğe Antioxidant Activity, Some Nutritional and Colour Properties of Vacuum Dried Strawberry Tree (Arbutus Unedo L.) Fruite(Termedia Publishing House Ltd, 2011) Orak, Hakime Hülya; Aktaş, Türkan; Yağar, Hülya; İşbilir, Şebnem Selen; Ekinci, Neslihan; Şahin, Füsun H.Background. The strawberry tree (Arbutus unedo L.) fruit contains a higher amount of nutrients and bioactive compounds than many other cultivated species, however, the edible use of this fruit is currently not widespread. In this study, the influences of vacuum drying have been investigated in terms of changing of some nutritional characteristics, antioxidant properties, and colour. Material and methods. Fruits were collected from Canakkale province in Turkey and next vacuum dried. Ethyl oleate and water blanching pre-treatments were applied to fruits before drying. Ascorbic acid, reducing sugar, minerals, colour, total phenolic content, DPPH radical scavenging activity, beta-carotene bleaching activity and HMF formation were determined. Results. The EO pretreatment shortened the drying time more than WB and gives a higher beta-carotene bleaching activity, lower HMF and higher yellowness and brightness of external colour characteristics. Conclusions. In this research, the effects of vacuum drying process on the colour, antioxidant activity and nutritional characteristics of fruit have been determined and it has been concluded that the strawberry tree fruit is assessable in food industry by drying due to rich nutritional components, antioxidant activity and attractive colour of the fruit.Öğe Antioxidant and antidiabetic activities of hexane extract of Genista januensis var. lydia(Bulgarska Akademiya na Naukite, 2022) Çakır, C.; Orak, Hakime Hülya; Argon, M.; Çalışkan, H.; Sabudak, Temine; Öztürk, M.; Güler, N.The genus Genista L. (Fabaceae) has been of interest for human beings since ancient times with its cosmopolitan distribution. Since ?-amylase and ?-glucosidase are the chief enzymes of diabetes mellitus, this study aims to study the ?-amylase and ?-glucosidase inhibitory potential and antioxidant activity of Genista januensis var. lydia grown in the Trakya region. To the best of our knowledge, there has been no study on G. januensis var. lydia up to date. The ?-amylase/?-glucosidase inhibitory activity and antioxidant activity of the hexane extract of G. januensis var. lydia were studied by spectroscopic in vitro experiments to search the potential relationships of both activities. © 2022 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.Öğe Antioxidant and Antimicrobial Activities of White, Green and Black Tea Extracts(Akademiai Kiado Zrt, 2013) Orak, Hakime Hülya; Yağar, Hülya; İşbilir, Şebnem Selen; Demirci, Ahmet Şükrü; Gümüş, TuncayIn the present study, the antioxidant and antimicrobial activities of three tea (Camellia sinensis) types (white tea WT, green tea - GT, and black tea - BT) were compared and the relationships between total phenolic, tannin and flavonoid contents were determined. Regardless of the assays used, the highest total phenolic content (313.3 +/- 1.41 mu g GAE/mg extract), total flavonoid (16.98 +/- 0.27 mu g QE/mg extract) and total tannin content (266.79 +/- 2.59 mu g TAE/mg extract) were determined in green tea extract, which also demonstrated the highest antioxidant capacity. Black tea extract showed the lowest phenolic content and antioxidant capacity. The EC50 value of DPPH scavenging activity was in the order of: ascorbic acid >GT>BHA>WT>BT>BHT. While the tea extracts exhibited antibacterial activity against Staphylococcus aureus, no inhibitory effects were observed against Escherichia coli and Salmonella enteritidis. All extracts exhibited antifungal activity against two aflatoxigenic moulds Aspergillus parasiticus NRRL 2999 and NRRL 465. The antibacterial activity of tea extracts decreased in the following order: GT>WT>BT DPPH scavenging activity strongly correlated with total phenolic content, reducing power, antimicrobial activity against S. aureus, A. parasiticus NRRL 2999, A. parasiticus NRRL 465 (P<0.05). These data suggest that green tea extract is more effective than white and black tea extracts as a potential source of natural antioxidants.Öğe Antioxidant Potential and Phenolic Compounds of Some Widely Consumed Turkish White Bean (Phaseolus vulgaris L.) Varieties(De Gruyter Open Ltd, 2016) Orak, Hakime Hülya; Karamac, Magdalena; Orak, Adnan; Amarowicz, RyszardThe antioxidant potential and phenolic compounds content were investigated in ten white bean varieties widely consumed in Turkey. Total phenolic contents of seeds varied between 0.33 and 0.63 mg GAE/g. The Trolox Equivalent Antioxidant Capacity (TEAC) determined by the ABTS assay and the Ferric-Reducing Antioxidant Power (FRAP) of bean varieties ranged from 3.50 to 5.17 mu mol Trolox/g seed and from 7.99 to 11.20 mu mol Fe2+-/g seed, respectively. Strong correlations were found between total phenolic content and FRAP (r=0.850) and between TEAC and FRAP (r=0.734). The antioxidant activity was investigated in a beta-carotene-linoleic acid model system, as well. Differences in the inhibition of emulsion oxidation by extracts of white bean varieties were slight. The RP-HPLC fingerprint analysis of extracts showed the presence of five dominant phenolic compounds which were described as ferulic or caffeic acids derivatives. Four of them significantly contributed to reducing power and antiradical activity against ABTS(center dot+) of extracts.Öğe BIOACTIVE COMPOUNDS, ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES OF TWO Cirsium SPECIES(Wydawnictwo Akad Rolniczej W Lublinie, 2019) Gülen, Dumrul; Şabudak, Temine; Orak, Hakime Hülya; Çalışkan, Hilmican; Özer, MerveIn the GC-MS analysis, 81 bioactive phytochemical compounds were identified in hexane extracts of Cirsium creticum and Cirsium italicum. Terpenoids constituted the main fractions of C. italicum (70.95%), while hydrocarbons were most abundant compounds of C. creticum (41.11%). The antibacterial activity and antifungal activity of extracts from two Cirsium species was tested using microdilution methods. According to the results of antibacterial activity, the highest inhibition effect of C. creticum was found on B. subtilis, E. coli and P. aeruginosa. The highest inhibition effect of C. italicum was found on B. subtilis. In the results of antifungal activity, the highest inhibition effect of C. creticum was found on P. chrysogenum and the highest inhibition effect of C. italicum was found on C. krusei. The present work is the first report on hexane extracts compounds of two Cirsium species as well as antibacterial and antifungal activities.Öğe Biological activity of new flavonoids and phenolic compounds from Cirsium italicum (Savi) DC.(Taylor & Francis Ltd, 2021) Şabudak, Temine; Çalışkan, Hilmican; Orak, Hakime Hülya; Özer, MerveCirsium italicum (Savi) DC. was researched for its phytochemicals for the first time. Two new biflavonoids, 5,5'',7''-trihydroxy-3,7-dimethoxy-4'-4'''-O-biflavone (3), (8,3',4',5'-tetrahydroxy-7-O-alpha-glucosyl isoflavone)-6-8''-(4'''-hydroxy-7''-O-alpha-glucosyl isoflavone) biflavone (7), a new isoflavone, 6-2'-dihydroxy-5-methoxy isoflavonone-4'-O-alpha-D-glucopyranoside (5) and a new phenolic compound, ethyl-(2,3-dihydroxy-5,6-dimethoxy-4-acetyl) benzoate (4), together with three known compounds (1), (2) and (6) were isolated from Cirsium italicum. Their structures were elucidated by extensive 1D and 2D NMR, and MS data analyses. All compounds isolated from C. italicum exhibited antioxidant activity. However, the highest activity of the EtOAc extract is considered to be responsible for compounds (1), (2) and (3) being the main compounds. [GRAPHICS] .Öğe Comparison of antioxidant activities of juice, peel, and seed of pomegranate (Punica granatum L.) and inter-relationships with total phenolic, Tannin, anthocyanin, and flavonoid contents(Korean Society Food Science & Technology-Kosfost, 2012) Orak, Hakime Hülya; Yağar, Hülya; İşbilir, Şebnem SelenIn this study, the antioxidant activities of juice, peel, and seed parts of pomegranate were investigated by using DPPH scavenging activity, beta-carotene bleaching method, reducing power, and metal chelating activity. Sample of pomegranates which are named Punica granatum L. cv. Hicaznar, genotype 19-121, genotype 17-67, and genotype 19-66 obtained from BATEM (West Mediterranean Agricultural Research Institute) in Anlalya. The EC50 values of DPPH scavenging activities in peel extracts (PE) had 23.4-fold higher than the juice extracts (JE), and the seed extracts (SE) had 2.3-fold higher than JE. The reducing power in peel extracts was found to be 4.7-fold higher than SE and 10.5-fold higher than the JE. The highest metal chelating capacity (37.22%) was determined in peel, while the lowest (7.151%) in seed. Generally, in peel, the total polyphenol, flavonoid, tannin contents, and in juice, the total polyphenol, anthocyanin, tannin contents, and acidity significantly affected to antioxidant activities.Öğe DETERMINATION OF ANTIOXIDANT ACTIVITIES OF STRAWBERRY TREE (Arbutus unedo L.) FLOWERS AND FRUITS AT DIFFERENT RIPENING STAGES(Wydawnictwo Akad Rolniczej W Lublinie, 2012) İşbilir, Şebnem Selen; Orak, Hakime Hülya; Yağar, Hülya; Ekinci, NeslihanIn this study, the antioxidant activities of Strawberry tree (Arbutus unedo L.) flowers at flowering period and fruits during ripening period were investigated, along with some ripening characteristics such as color, reducing sugar and acidity. Antioxidant activity characteristics were investigated using by the methods of DPPH (1,1-Diphenyl-2-picryl-hydrazyl) scavenging activity, beta-carotene bleaching, reducing power, metal chelating capacity, superoxide anion scavenging and hydrogen peroxide scavenging activity in water, ethanol and methanol extracts. The total phenolic content of flowers in water extracts was found to be 232.38 +/- 7.19 mg GAE . g(-1) extract, and the DPPH activity was 81.3 +/- 0.49% at 50 mu g.ml(-1) concentration. In the ripening stages, the fully red fruits were determined higher antioxidant capacity than green and yellow fruits, except H2O2 scavenging activity which was highest in green fruit. In correlation study, the highest relationship was found between total phenolic content with reducing power (r(2) = 0.987**, P < 0.01), while the lowest with H2O2 scavenging activity (r(2) = 0.519*, P < 0.05).Öğe Determination of antioxidant activities of strawberry tree (Arbutus unedo L.) flowers and fruits at different ripening stages(Wydawnictwo Akad Rolniczej W Lublinie, 2012) İşbilir, Şebnem Selen; Orak, Hakime Hülya; Yağar, Hülya; Ekinci, NeslihanIn this study, the antioxidant activities of Strawberry tree (Arbutus unedo L.) flowers at flowering period and fruits during ripening period were investigated, along with some ripening characteristics such as color, reducing sugar and acidity. Antioxidant activity characteristics were investigated using by the methods of DPPH (1,1-Diphenyl-2- -picryl-hydrazyl) scavenging activity, ?-carotene bleaching, reducing power, metal chelating capacity, superoxide anion scavenging and hydrogen peroxide scavenging activity in water, ethanol and methanol extracts. The total phenolic content of flowers in water extracts was found to be 232.38 ± 7.19 mg GAE · g-1 extract, and the DPPH activity was 81.3 ± 0.49% at 50 ?g·ml-1 concentration. In the ripening stages, the fully red fruits were determined higher antioxidant capacity than green and yellow fruits, except H2O2 scavenging activity which was highest in green fruit. In correlation study, the highest relationship was found between total phenolic content with reducing power (r2 = 0.987**, P < 0.01), while the lowest with H2O2 scavenging activity (r2 = 0.519*, P < 0.05).Öğe Determination of antioxidant properties of lyophilized olive leaf water extracts obtained from 21 different cultivars(Korean Society Food Science & Technology-Kosfost, 2012) Orak, Hakime Hülya; İşbilir, Şebnem Selen; Yağar, HülyaThe objective of this study was to compare the antioxidant activities of lyophilized olive leaf water extracts (OLWExtracts) from 21 different olive tree cultivars. The highest total phenolic content (102.69 +/- 1.63 mu g GAE/mg extract) was found in cultivar 'Esek Zeytini' and the lowest content (78.52 +/- 2.18 mu g GAE/mg extract) was in 'Uslu'. The highest total flavonoid content (12.47 +/- 0.12 mu g QE/mg extract) obtained from 'Frantoio'. According to EC50 values, 'Domat' have the highest DPPH scavenging activity (EC50 =59 mu g/mL), reducing power (EC50 =180 mu g/mL), and superoxide radical scavenging capacity (EC50=44 mu g/mL). While, 'Ascolana' showed the highest metal chelating capacity (EC50=163 mu g/mL), 'Memecik' showed the highest hydrogen peroxide (H2O2) scavenging capacity. Research results exhibited that OLWExtracts are comparable to synthetic antioxidants. The correlation study showed that while total phenolic content correlated with DPPH scavenging activity and reducing power (p < 0.01), total flavonoid content correlated with metal chelating and superoxide radical scavenging capacity (p < 0.05).Öğe Determination of Glucose and Fructose Contents of Some Important Red Grape Varieties by HPLC(Asian Journal Of Chemistry, 2009) Orak, Hakime HülyaGlucose, fructose and sucrose contents of 24 different red grape varieties (Vitis vinifera), grown in Turkey, were determined by using high-performance liquid chromatography. Among analyzed samples glucose content of varieties varied from 5.98 % (Alicante) to 12.21 % (Gewurztraminer) and the fructose content was ranged between 5.93% (Bogakere) and 12.66 % (Md.Jean Mattihas). Sucrose was determined in three different varieties which were Kalecik karasi, Md.Jean Mattihas and Cinsault.Öğe Effects of Different Drying Methods on Drying Kinetics and Color Parameters of Strawberry Tree (Arbutus unedo L.) Fruit(Namık Kemal Üniversitesi, Ziraat Fakültesi, 2013) Aktaş, Türkan; Orak, Hakime Hülya; Şahin, Füsun Hastürk; Ekinci, NeslihanChanging of drying kinetics and color parameters of strawberry tree fruits that were notreated (NPT), ethyl oleate pretreated (EO) and water blanched at 80 ºC (WB) for one minute were evaluated during hot air drying at 50 °C, 60 °C and 70 °C and vacuum drying at 60 °C, 70 °C and 80 °C for halved and whole fruits. In addition to drying behaviors of strawberry tree fruits at different drying methods, triLstimulus color parameters such as Hunter L*, a* and b* values were measured. HMF (formation of 5Lhydroxymethylfurfural) occurring was also determined due to drying process. EO pretreatment was found highly effective on decreasing of drying time both for hot air drying and vacuum drying processes while WB pretreatment found to be more effective to keep color properties of strawberry tree fruits. Color properties were found to be better in half samples compared to whole dried fruits. HMF values verified this result. Hot air drying process caused to shorter drying time. High drying air temperatures (70 ºC for hot air drying and 80 ºC for vacuum drying) for all samples caused to occur HMF while HMF occurred in only whole dried NPT and WB treated fruits for hot air drying process at 60 ºC. All these data show that hot air drying method at 60 ºC drying temperature and, halving of fruits before drying can be proposed for drying of strawberry tree fruits in respect of drying kinetics, color and HMF values.Öğe Effects of different drying techniques on some nutritional components of tomato (lycopersicon esculentum)(2010) Hastürk Şahin, Füsun; Ülger, Poyraz; Aktaş, Türkan; Orak, Hakime HülyaIn this research, four drying techniques were applied for tomato (Lycopersicon escuientum) namely, hot air drying using tunnel type dryer, vacuum drying, freeze drying and sun drying and changes of food components of the vitamin C, lycopene, sodium (Na), and potassium (K) content were examined. As a result of drying applications, the initial moisture content of 94.73% of fresh tomato, dried tomato samples were found to range from 9.33% to 11.11%. Losses of nutritional contents of hot air dried samples increased with rising drying temperature. Loss of lycopene and Potassium content of vacuum dried samples at 75 °C were found lower compared to hot air dried samples at the same drying temperature. On the other hand increasing of drying time for vacuum drying increased loss of vitamin C. Vitamin C and lycopene content of sun dried samples were found higher compared to those of hot air dried and vacuum dried samples. Freeze drying method gave the best results in respect of all nutritional value criteria.
