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    Bazı yerli ve yabancı ekmeklik buğday genotiplerinde kahverengi pasa dayanıklılığın moleküler belirteçler ile belirlenmesi
    (Tekirdağ Namık Kemal Üniversitesi, 2023) Nasirian, Hazal; Bilgen, Behiye Banu
    Geçmişten günümüze buğday insan beslenmesinde önemli bir besin maddesi olmuştur ve gelecekte de bu önemini korumaya devam edecektir. Buğdayın; adaptasyon yeteneğinin yüksek, işlemesinin kolay ve az maliyetli olmasından dolayı dünyanın birçok bölgesinde üretimi gerçekleştirilebilmektedir. Geniş alanlarda yayılış göstermiş olan buğday, sadece insan beslenmesinde değil hayvan beslenmesinde ve endüstri alanında da kullanılmaktadır. Birçok alanda kullanılan buğdayın gelecekteki talebi karşılayabilmesi için verim kayıplarının önüne geçilmesi ve birim alandan elde edilen veriminin arttırılması gerekmektedir. Buğdayda verim kayıplarının nedenlerinden biri de kahverengi pas hastalığıdır. Bu kayıpların engellenebilmesi ve verimin arttırılabilmesi için yeni çeşitlerin ıslah edilmesi gerekmektedir. Çeşitlerin ıslahında, moleküler destekli ıslah ile klasik ıslah metotlarının birlikte kullanılması daha etkili ve hızlı sonuçlar alınmasını sağlayabilmektedir. Yapılan tez çalışmasında buğdayda kahverengi pas dayanıklılık geni olan Lr gen bölgelerinden; Lr1, Lr9, Lr10, Lr13, Lr14a, Lr19, Lr21, Lr22a, Lr24, Lr34, Lr35, Lr37, Lr39, Lr46, Lr47, Lr49 ve Lr58 gen bölgeleri çalışılmıştır. Çalışma da PCR optimizasyonu sağlanan Lr13, Lr14a, Lr19, Lr22a, Lr24, Lr34, Lr37 ve Lr47 gen bölgeleri ile 96 buğday genotipi kahverengi pasa dayanıklılık açısından test edilmiştir. Çalışmadan elde edilen verilerin kahverengi pasa dayanıklılık genleri içeren çeşitlerin buğday ıslah programlarında kullanılmasına önemli bilgiler sağlayacağı ve etkili ıslah programlarının oluşturulmasında önemli paya sahip olacağı düşünülmektedir.
  • Küçük Resim Yok
    Öğe
    Brown rust resistance screening and molecular characterization of wheat cultivars by molecular markers
    (Tubitak Scientific & Technological Research Council Turkey, 2023) Bilgen, Behiye Banu; Yuruk, Busra; Nasirian, Hazal
    Triticum aestivum L. is one of the major strategic crops for many reasons such as being the basic material for the majority of nutrients, being a source of energy, a source of protein, and also other organic and inorganic compounds for both humans and animals. The genetic characterization is useful for designing plant breeding programs for many plants, including wheat. The selection of genotypes resistant to stress (biotic and abiotic) conditions is another important point for the design of plant breeding programs. Wheat brown (leaf) rust is one of the major significant biotic stress factors. In this study, marker analysis for brown rust resistance for Lr13, Lr14a, Lr19, Lr22a, Lr24, Lr34, Lr37, and Lr47 genes was performed, and the genetic characterization of 96 wheat genotypes was determined by 8 SSR and 4 ISSR loci. Eighteen alleles were determined for SSR loci (polymorphism ratio of 37.5%) and a total of 33 polymorphic bands (polymorphism ratio of 82.5%) were determined for ISSR loci. The mean Shannon Index was estimated as I (SSR) = 0.298 and I (ISSR) = 0.346. The mean gene diversity for SSR and ISSR was estimated as 0.407 and 0.246, respectively. The genetic similarity value based unweighted pair group method with arithmetic was used to form the dendrogram and it was remarked wheat varieties were grouped into two main clusters. The STRUCTURE analysis also separated the studied wheat genotypes into two groups. The results reveal the genetic structure, the genetic diversity, and the presence of resistance alleles of used wheat varieties, and the selection of genetically diverse and leaf rust-resistant genotypes can serve as valuable parents for future wheat breeding or improving breeding programs.
  • Küçük Resim Yok
    Öğe
    Brown rust resistance screening and molecular characterization of wheat cultivars by molecular markers
    (2023) Bilgen, Behiye Banu; Yürük, Büşra; Nasirian, Hazal
    Triticum aestivum L. is one of the major strategic crops for many reasons such as being the basic material for the majority of nutrients, being a source of energy, a source of protein, and also other organic and inorganic compounds for both humans and animals. The genetic characterization is useful for designing plant breeding programs for many plants, including wheat. The selection of genotypes resistant to stress (biotic and abiotic) conditions is another important point for the design of plant breeding programs. Wheat brown (leaf) rust is one of the major significant biotic stress factors. In this study, marker analysis for brown rust resistance for Lr13, Lr14a, Lr19, Lr22a, Lr24, Lr34, Lr37, and Lr47 genes was performed, and the genetic characterization of 96 wheat genotypes was determined by 8 SSR and 4 ISSR loci. Eighteen alleles were determined for SSR loci (polymorphism ratio of 37.5%) and a total of 33 polymorphic bands (polymorphism ratio of 82.5%) were determined for ISSR loci. The mean Shannon Index was estimated as I (SSR) = 0.298 and I (ISSR) = 0.346. The mean gene diversity for SSR and ISSR was estimated as 0.407 and 0.246, respectively. The genetic similarity value based unweighted pair group method with arithmetic was used to form the dendrogram and it was remarked wheat varieties were grouped into two main clusters. The STRUCTURE analysis also separated the studied wheat genotypes into two groups. The results reveal the genetic structure, the genetic diversity, and the presence of resistance alleles of used wheat varieties, and the selection of genetically diverse and leaf rust-resistant genotypes can serve as valuable parents for future wheat breeding or improving breeding programs.