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    Antioxidant Activity and Total Phenolic Content of Gagea fibrosa and Romulea romiflora
    (Jihad Daneshgahi, 2011) Mammadov, Ramazan; Ili, Pınar; Ertem Vaizogullar, Havser; Makasçı Afacan, Ayşe
    Free radicals involved in a number of diseases due to the oxidative damage to DNA, lipids, and proteins and which can result in failure of cellular functions. Dried plant bulbs and leaves methanolic and ethanolic extracts were prepared. Total antioxidant activities of the methanolic extracts of Romulea ramiflora and Gagea fibrosa were determined using beta-carotenlinoleic acid assay. Romulea-Bulb-Ethanol (RBE) extract showed the highest (89.64 +/- 1.25%) but, Romulea-Leaves-Ethanol (RLE) extract showed the lowest (33.23 +/- 1.13%) antioxidant activity. The lowest antioxidant activity of the Gagea leaves extract (GLE - Gagea-Leaves-Ethanol, 46.18 +/- 0.09%) was higher (p < 0.05) then Romulea Leaves Extract (RLE -33.23 +/- 1.13%). This may be related with the structure of the plant's leaves cells. The DPPH radical scavenging assay was used to determine the antiradical activities. Gagea-Leaves-Methanol extract (GLM -61.16 +/- 1.46%) has higher DPPH radical scavenging activity than Romulea-Bulb-Methanol extract (RBM -51.27 +/- 0.94%). Generally leaf extracts have the highest free radical scavenging activity. The total phenolic content of extracts was determined using to the Folin-Ciocalteu method. RBE has the highest phenolic content (74,12 +/- 4,6 mg/100 g plant extract). R. ramiflora and G. fibrosa have the antioxidant and free radical scavenging activities. These properties are due to the number of the hydroxyl groups of the phenolic compounds, but not amount of them.
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    Determination of Antioxidant Activities of Different Urginea maritima (L.) Baker Plant Extracts
    (Jihad Daneshgahi, 2010) Mammadov, Ramazan; Makasçı Afacan, Ayşe; Uysal-Demir, Derya; Gork, Çiğdem
    The subject handled in this study is the determination of antioxidant activities of extracts obtained from the Urginea maritima (L.) Baker plant's leaves and tubers. Extractions which were obtained using various solvents as methanol, ethanol, acetone and benzine. The antioxidant activity of the extracts was determined using beta-karotene-linoleic acid system. Moreover, the free radical scavenging activity values were tested with DPPH. The concentrations of phenolic compounds in all extracts of UM, expressed as microgram of pyrocatechol equivalents (PEs) were determined with Folin-Ciocalteu reagent (FCR). The results demonstrated that the highest antioxidant activity in the U. maritima (L.) Baker showed in the tubers with ethanol solvent (%72,67) and the lowest in the benzine extract from the tubers (%31,12). The free radical scavenging activity of the methanol extract from the tubers of the U maritima (L.) Baker plant (%66.89) was found lower than the BHT value (%91.12) but still the highest. However, the amount of total phenolic compounds of acetone extract of U. maritime (L.) Baker tubers was 6 times more than ethanol extract. This study demonstrated that U maritime (L.) Baker species have high rate antioxidant capacity.