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Öğe Determination of the association of GHR/Alul gene polymorphisms with milk yield traits in Holstein and Jersey cattle raised in Turkey(Copernicus Gesellschaft Mbh, 2021) Çobanoglu, Özden; Kul, Ertuğrul; Gürcan, Eser Kemal; Abaci, Samet Hasan; Çankaya, SonerThis research was carried out to determine the effect of a specific single nucleotide polymorphism (SNP) region in exon 10 of the growth hormone receptor (GHR) gene on milk production traits in Jersey and Holstein cows raised in Turkey. Milk samples were recorded as a test day milk yield (TDMY) and an adjusted based 305 d milk yield (305-DMY). Also, milk component traits were detected. Based on the scope of this study, a total of 748 dairy cows, including 305 Holsteins raised in the Marmara Region and 163 Holstein and 280 Jersey raised in the Black Sea Region, were genotyped for the GHR gene using the RFLP-PCR technique. Jersey cows carrying the GG genotype (5.24 %) were associated with higher fat content (P < 0.05). Jersey cows with GG and AG also had a higher protein content (3.44 % and 3.38 %, respectively) (P < 0.05). Similarly, the protein content was the highest in Holstein cows with the GG genotype (3.46 %) (P < 0.01), whereas Holstein cows having AA genotypes displayed higher TDMY (24.64 kg/d) (P < 0.05) and 305-DMY (8472.4 kg) (P < 0.01). The estimated increase in milk protein and fat contents due to the G allele was 0.07 % and 0.22 % in the Jersey breed, respectively. On the other hand, allele A was highly related to an increase in protein yield and 305-DMY of 0.04 and about 675 kg in the Holstein breed, respectively. The GHR gene should be considered as a potential candidate gene in marker-assisted selection programs to improve the performance of milk and related traits in Turkey dairy cattle populations.Öğe Determination of the genetic polymorphism for DGAT1 gene in Holstein, Jersey and native cattle breeds of Turkey(Agricultural Research Communication Centre, 2019) Gürcan, Eser Kemal; Çobanoglu, Özden; Kul, Ertuğrul; Abacı, Samet Hasan; Çankaya, SonerThis study was to determine the genetic polymorphism in terms of the DGAT1 gene among Holstein and Jersey and indigenous Native Black, East Anatolian Red and Grey Steppe in Turkey. The frequencies of K allele for the DGAT1 gene were calculated as 0.59, 0.54, 0.53, 0.62, 0.52, and 0.54 for Jersey, Holstein (Black Sea Region), Holstein (Marmara Region), and Grey Steppe, East Anatolian Red, and Native Black breeds, respectively. The F-IS values of the populations for the DGAT1 gene were detected all negative values. In overall population, F-IS value was found as 76%. The expected deviations from the Hardy-Weinberg Equilibrium in terms of the DGAT1 locus were found significant in these six different populations (P<0.01). The genetic distance values among the populations were calculated between 0.0001 and 0.0089. Based on the cluster analysis, Holstein, Native Black and East Anatolian Red were located in close cluster; however, Jersey and Grey Steppe were grouped in different cluster.Öğe Effects of lactation month and season on test-day milk yield and milk components in Holstein cows(Agricultural Research Communication Centre, 2017) Çobanoglu, Özden; Gürcan, Eser Kemal; Çankaya, Soner; Kul, Ertuğrul; Abaci, Samet Hasan; Ülker, MehmetThis study aims to determine the correlations between the test-day milk yield (TDMY) and the fat and protein content as well as the fat and protein yields in Holstein dairy cows and to investigate the effects of lactation period and sampling season on these parameters. The study material consists of 1.380 records of a total of 151 head of Holstein dairy cows raised on a private farm in Samsun province. In the study, the mean TDMY, fat content, protein content, fat yield and protein yield were recorded as 20.6 +/- 5.10 kg, 3.97 +/- 0.734%, 3.23 +/- 0.276%, 0.81 +/- 0.210 kg and 0.66 +/- 0.160 kg, respectively. The effects of lactation period and sampling season on the TDMY, fat content, protein content, fat yield and protein yield were found statistically significant (P<0.01). Statistically significant negative correlations were determined between the TDMY and the fat and protein content, but positive and statistically significant (P<0.01) correlations between the TDMY and the fat and protein yields. In conclusion, it might be stated that the effects of lactation period and sampling season should also be taken into account to improve milk yield and milk components.Öğe Estimation of genetic and environmental parameters for milk traits in jersey cows(2018) Kul, Ertuğrul; Abacı, Samet Hasan; Çobanoğlu, Özden; Gürcan, Eser Kemal; Çankaya, SonerThe aim of this study was to estimate the genetic parameters and environmental factors for testday milk yield (TDMY), lactation milk yield (LMY), 305-day milk yield (305-DMY), milk fatpercentage (FP) and milk protein percentage (PP) of Jersey cows raised on KaraköyAgricultural State Farm in Samsun province of Turkey. The data were obtained from 279 milkyield records of 170 heads cows calved from 2011 to 2013 years. Parity, calving season andcalving year were considered as fixed effects. Heritability, additive genetic variance andphenotypic variance were estimated by multiple trait derivative free restricted maximumlikelihood (MTDFREML) with the animal model. Variance analysis results showed thatexcept for FP, the effects of parity and calving year on TDMY, LMY, 305-DMY and PP weresignificantly important (P<0.05). However, calving season did not affect TDMY, LMY, 305-DMY, FP and PP (P>0.05). Heritability estimates for TDMY, LMY, 305-DMY, FP and PPwere 0.38, 0.30, 0.28, 0.19, and 0.36, respectively. Repeatability for TDMY, LMY, 305-DMY, FP and PP were ranged from 0.35 to 0.45. The results indicate that these traits can beused as a selection criteria in genetic improvement of Jersey cattle this herd.Öğe Genetic Polymorphism of STAT1 and STAT5A Genes in Holstein, Jersey, and Indigenous Cattle Breeds in Turkey(Kafkas Univ, Veteriner Fakultesi Dergisi, 2020) Çobanoglu, Özden; Kul, Ertuğrul; Abaci, Samet Hasan; Gürcan, Eser Kemal; Çankaya, SonerThis study aimed to determine genetic polymorphism in STAT1 and STAT5A genes for dairy cattle and some native cattle breeds in Turkey. 283 Jersey and a total of 472 Holstein cows from two different herds and 93 Grey Steppe, 85 Anatolian Black Cattle, and 66 East Anatolian Red cattle were used in this research. Generally, C allele gene frequency was higher than T allele for STAT1 in all breeds whereas C allele gene frequency was detected higher than G allele for STAT5A in Jersey and East Anatolian Red. On the other hand, G allele gene frequency was higher than C allele in Holstein, Grey Steppe, and Anatolian Black Cattle breeds. The expected deviations from the Hardy-Weinberg Equilibrium were significant only for Jersey breeds for STAT1 gene. Meanwhile, the expected deviation from equilibrium was also significantly different for Holstein in Black Sea Region (BSR), Anatolian Black Cattle and Grey Steppe for the STAT5A gene. FIS values were determined to STAT1 gene as negative for all breeds except for Holstein in Marmara Region (MR). Similarly, this value was determined to STAT5A gene as positive for all breeds except for Holstein in BSR. The genetic distances for two loci were calculated between 0.0029 and 0.1599 among all populations. Depending on the cluster analysis, Holstein in BSR and MR, Anatolian Black Cattle, East Anatolian Red were closely clustered to each other, while Grey Steppe and Jersey were located in completely different clusters. As a conclusion, based on the detected genetic diversity in STAT1 and STAT5A genes, it is possible to make a genetic improvement among bovine breeds raised in Turkey.Öğe Influence of Some Environmental Factors on Milk Yield and Milk Components Traits in Jersey Cows(Parlar Scientific Publications (P S P), 2019) Çobanoglu, Özden; Kul, Ertuğrul; Gürcan, Eser Kemal; Çankaya, Soner; Abaci, Samet Hasan; Ülker, MehmetThis study was conducted to assess the effects of stage of lactation, parity and sampling season on milk yield and milk components in Jersey cows in Karakoy State Farm, Turkey. The data set consisted of 2657 milk records of 204 Jersey cows collected from September 2011 to December 2013. For this purpose, test day milk yield (TDMY) records and milk samples were taken once a month for 10-month period after parturition. The effect of stage of lactation, parity and sampling season on TDMY, fat percentage, protein percentage, fat yield and protein yield were found statistically significant (P<0.01). TDMY were correlated with fat percentage (-0.235) and protein percentage negatively (-0.254) but with fat yield (0.755) and protein yield positively (0.950) (P<0.01). In conclusion, these results suggest animal breeders that the effects of lactation stage, parity and sampling season may be taken into account in husbandry management to improve the milk yield and milk quality in Jersey cows.Öğe Jersey İneklerinde STAT5A Geninin Süt Verimi ve Bileşenleri Üzerine Etkisinin Araştırılması(2014) Çankaya, Soner; Ülker, Mehmet; Çobanoğlu, Özden; Gürcan, Eser Kemal; Kul, Ertuğrul; Abacı, Samet HasanBu araştırmada, süt verim ve bileşenleri üzerine etkisi olduğu düşünülen signal transducer and activator of transcrption factor (STAT) ailesine üye STAT5A geninin, Jersey ırkı süt sığırlarında süt verimi ve bileşenleri (yağ, protein) üzerine etkisi araştırılmıştır. Araştırmada materyal olarak Samsun Karaköy Tarım İşletmesinde yetiştirilen 100 baş Jersey ineği kullanılmıştır. İneklerden ayda bir kez olmak üzere 12 ay boyunca süt örnekleri alınmış ve bu örnekler ultrasonik süt analiz cihazı yardımıyla yağ ve protein oranı bakımından analiz edilmiştir. Ayrıca süt örneklerinin alındığı tarihlerde test günü süt verim (TGSV) kayıtları da alınmıştır. İneklerden alınan kan örneklerinden DNA izolasyonu sonucunda PCR-RFLP yöntemiyle, 8.ekzonda 12195. pozisyonda C/G polimorfizmi belirlenmiştir. Genotip dağılımı ise 69 CC, 28 CG ve 3 GG şeklinde olmuştur. Allel frekansları ise C alleli için 0.83 ve G alleli için 0.17 olarak belirlenmiştir. STAT5A geninde belirlenen polimorfizm sonucunda genotipler arasında test günü süt verimi (TGSV), 305 gün süt verimi (305 GSV), süt yağ ve protein oranı, süt yağ ve protein verimi bakımından önemli bir fark olmadığı tespit edilmiştir (P>0,05) . Sonuç olarak Karaköy Tarım İşletmesinde yetiştiriciliği yapılan Jersey ineklerinde STAT5A (SNP 12195) geni açısından süt verim ve bileşenleri açısından herhangi bir fark tespit edilememiştir.