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    The use of HPLC in metabolomics research
    (Nova Science Publishers, Inc., 2023) Karan, Tunay; Vicil, Sinan
    Metabolomics detects, identifies, and quantifies small moleculecontaining metabolites in cells, tissues, and physiological fluids through high-performance yielding technologies. Metabolomics is an integral part of biotechnology, which includes drug activity investigations, enzyme-to-substrate interactions, biomarker identifications, and metabolomic pathway analyses. Metabolite profiling can be achieved cost-effectively in a short time with high precision and specificity through High-Performance Liquid Chromatography (HPLC), one of the analytical methods. Metabolomics research is seen in different fields, including nutrition, toxicology, environmental pollution, drug research and development, and a broad spectrum of biochemical and industrial analyses. Here in this chapter, fundamental basics about HPLC will be given, and the use and importance of HPLC in metabolomics will be highlighted. © 2023 Nova Science Publishers, Inc. All rights reserved.