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Öğe Differential loss of PRUNE2 in colorectal adenocarcinoma tissues(Springer Science and Business Media Deutschland GmbH, 2025) Gunduz, Ihsan; Karaboga, Ihsan; Bozgeyik, Esra; Ozturk, Ozlem; Beyaz, Yuksel; Duran, Yasin; Polat, Fatin RustuColorectal cancer is of the most prevalent and deadliest cancer in the world. Cumulative evidence suggests that PRUNE is a tumor suppressor gene and plays a significant role in the development and progression of human cancers. However, its role during colorectal cancer development remains elusive. In the present study, we aimed to determine the role of PRUNE2 and its diagnostic significance in colorectal cancer. A total of 70 patients with colorectal cancer and 30 control subjects were included in the present study. All patients were confirmed to be colorectal adenocarcinoma. PRUNE2, P53, and PCNA immunoreactivity of patients were determined using standard immunohistochemistry staining techniques. Notably, PRUNE2 was identified to be significantly downregulated in colorectal cancer patients compared to the control group (p < 0.0001). Almost no immunoreactivity was observed in tumor samples compared to healthy colon tissues, where significant immunoreactivity was observed. Also, both P53 and PCNA were significantly higher in patients with colorectal cancer (p < 0.0001). Overall, our results suggest that PRUNE is a reliable and robust biomarker for the differential diagnosis of colorectal cancer and can be used for future diagnostic applications together with other immunological biomarkers such as PCNA. © The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2025.Öğe Prenatal immobility stress: Relationship with oxidative stress, inflammation, apoptosis, and intrauterine growth restriction in rats(Wiley, 2023) Kaya, Sinem Albayrak; Okuyan, Hamza Malik; Erboga, Zeynep Fidanol; Guzel, Savas; Yilmaz, Ahsen; Karaboga, IhsanBackgroundPrenatal stress is a significant risk factor affecting pregnant women and fetal health. In the present study, we aimed to investigate the effect of immobility stress at different periods of pregnancy on oxidative stress, inflammation, placental apoptosis and intrauterine growth retardation in rats.MethodsFifty adult virgin female Wistar albino rats were used. Pregnant rats were exposed to 6 h/day immobilization stress in a wire cage at different stages of pregnancy. Groups I and II (Day 1-10 stress group) were sacrificed on the 10th day of pregnancy, and Group III, Group IV (10-19th-day stress group), and Group V (1-19th-day stress group) were sacrificed on the 19th day of pregnancy. Inflammatory cytokines, including interleukin-6 (IL-6) and interleukin-10 (IL-10), serum corticotropin-releasing hormone (CRH), and corticosterone levels were measured by enzyme-linked immunosorbent assay. Malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels in the placenta were spectrophotometrically measured. Histopathological analyses of the placenta were evaluated by hematoxylin and eosin staining. Tumor necrosis factor-alpha (TNF-a) and caspase-3 immunoreactivity in placenta tissues were determined by the indirect immunohistochemical method. Placental apoptosis was determined by the TUNEL staining method.ResultsWe found that the immobility stress during pregnancy significantly increased serum corticosterone levels. Our results showed that the immobility stress diminished the number and weight of fetuses in rats compared to the non-stress group. The immobility stress caused significant histopathological changes in the connection zone and labyrinth zone and increased placental TNF-a and caspase-3 immunoreactivity and placental apoptosis. In addition, immobility stress significantly increased the levels of pro-inflammatory IL-6 and MDA and caused a significant decrease in the levels of antioxidant enzymes such as SOD, CAT, and anti-inflammatory IL-10.ConclusionsOur data suggest that immobility stress causes intrauterine growth retardation by activating the hypothalamic-pituitary-adrenal axis and deteriorating placental histomorphology and deregulating inflammatory and oxidative processes.Öğe Royal jelly attenuates gastric mucosal injury in a rat ethanol-induced gastric injury model (vol 47, pg 8867, 2020)(Springer, 2024) Duran, Yasin; Karaboga, Ihsan; Polat, Fatin Rustu; Polat, Elif; Erboga, Zeynep Fidanol; Ovali, Mehmet Akif; Oztopuz, Rahime Ozlem[Abstract Not Available]Öğe The effect of sucrose consumption on eating behavior and depression during morphine withdrawal period in rats(Ios Press, 2024) Aslan, Serkan; Koksalb, Eda; Karaboga, IhsanBACKGROUND: Sugary drink and junk food consumption increases during the withdrawal period, leading to subsequent psychological and metabolic alterations. OBJECTIVES: We aimed to investigate the relationship of sucrose consumption with serum insulin levels, leptin levels, brain Dopamine-2 receptor (D2R) expression, food consumption, and anxiety-depression findings in morphine-withdrawal rats. METHODS: Thirty-six male Wistar albino rats were divided into six groups: Control, sucrose-free, 5% sucrose, 10% sucrose, 20% sucrose, and an addiction test. Saline was intraperitoneally injected to the control group, and morphine was intraperitoneally injected to the other groups for 14 days. After 14 days, naloxone was administered to the addiction test group, and addiction symptoms were observed and this group was sacrificed on the same day. Other groups were fed ad libitum with different concentrations of sucrose solution for one week. Behavioral parameters were evaluated at the end of the experiment. Leptin and insulin concentrations in serum and D2R levels in brain tissues were detected using an enzyme-linked immunosorbent assay. D2R concentrations in brain tissues were evaluated utilizing immunohistochemistry. RESULTS: We observed decreased food consumption and increased fluid consumption in rats that consumed sucrose water during the withdrawal period. The level of depression and binge eating behavior was elevated in groups consuming sucrose, and the 10% sucrose group had the highest carbohydrate consumption and anxiety levels. In addition, the 10% sucrose group had the lowest brain D2R expression. The leptin level was highest in the 20% sucrose group. CONCLUSIONS: These results show the possible effects of sugary drinks consumed during the withdrawal period.Öğe Urotensin-II Prevents Cartilage Degeneration in a Monosodium Iodoacetate-Induced Rat Model of Osteoarthritis (vol 28, 140, 2022)(Springer, 2024) Terzi, Menderes Yusuf; Okuyan, Hamza Malik; Karaboga, Ihsan; Gokdemir, Cemil Emre; Tap, Duygu; Kalaci, Aydiner[Abstract Not Available]
