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    The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1?) expression in rabbit cornea
    (2019) Ertekin, Yusuf Haydar; Arıkan, Sedat; Karaboğa, İhsan
    Introduction: To investigate the effect of ozone (O3) therapy on cornea subjected to systemically used high dose methylprednisolone (MP) ina rabbit model.Methods: Twenty-four New Zealand White adult male rabbits were randomly divided into three equal groups as containing eight animals. Thefirst group (n = 8) was used as the control group and nothing was applied to them, whereas the other 2 groups named as steroid groups weresubjected to IM methylprednisolone injection at a dose of 20 mg/kg/day for three days. After three days of MP administration, only the third groupwas treated with 50-µg/mL O3 (20 mL O3) through the rectal insufflation for 14 sessions. The histopathological examination of corneas of threegroups were made, and they were also assessed regarding the expression of hypoxia-inducible factor-1 ? (HIF-1?).Results: It was determined that systemically administered high dose MP caused erosion and necrosis in corneal epithelium and stromaldisintegrations in corneal stroma in steroid groups (Group 2 and Group 3). In the MP + O3 group (Group 3), the histopathological findings weremild. The expression of HIF1-? in the cornea of Group1 (control group), Group 2 (MP), and Group 3 (MP-O3) was measured as, 17.9±9.6%,3.1±1.0% and 6.4±1.9% respectively.Conclusions: MP and MP-O3 therapy decreased HIF-1a expression in rabbit cornea in both intervention groups. Between these two groups,HIF-1? expression remained relatively high in the MP-O3 group than in the MP group alone.