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    Effects of asprosin levels and selected biochemical parameters on conception in repeat breeder cows
    (Polish Soc Veterinary Sciences Editorial Office, 2024) Yenilmez, Kudret; Arslan, Sezai; Dogan, Hasan; Vicil, Sinan
    The purpose of this study was to investigate the effects of asprosin levels and various biochemical parameters (NEFA, BHBA, glucose, albumin, globulin, total protein, ALT, AST, and total bilirubin) on conception in repeat breeder (RB) cows. The study included sixty RB cows that had been inseminated at least three times, but had not been pregnant and had no gynecological abnormalities, as assessed by ultrasonography. From the sixty cows, two groups were selected at random: pregnant (Group I, n = 10) and non -pregnant (Group II, n = 10) animals. After estrus detection, a 10 mL blood sample was collected from each animal's tail vein. All animals were inseminated 12 hours after the start of estrus. The blood samples were centrifuged at 3000 rpm for 15 minutes, and the serum was extracted and stored at -80 degrees C until analysis. On the 45 th day after insemination, ultrasonography was used to examine the pregnancy. Asprosin levels were measured using ELISA, and biochemical tests (NEFA, BHBA, glucose, albumin, globulin, total protein, ALT, AST, total bilirubin) were performed with an autoanalyzer. Non -pregnant RB cows had considerably lower levels of asprosin (p < 0.05) than pregnant RB cows. When other biochemical parameters (BHBA, NEFA, glucose, albumin, ALT, AST, total bilirubin, total protein and globulin) were compared, there was no significant difference between the groups (p > 0.05). Thus, significantly elevated levels of asprosin in pregnant RB cows suggested that asprosin could play a role in RB cow conception. In addition, there was a positive correlation between asprosin levels and AST, total protein, and globulin levels in pregnant RB cows.
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    Factors influencing ovum pick-up technique results in cattle
    (The Chamber of Veterinary Surgeons, Istanbul, 2024) Dogan, Hasan; Yenilmez, Kudret
    The Ovum Pick-Up (OPU) technique, which is used in in vitro embryo production (IVP) to retrieve immature oocytes from live donor animals, is one of the most important biotechnological procedures used in cattle breeding. The most important advantage of this technology is that it allows for the reproducible retrieval of immature oocytes from living donor animals. It is particularly useful in dairy cattle breeding to address infertility issues and boost the production of superior animals with high genetic value. The OPU technique offers several advantages, including its applicability to cows ranging from six-month-old calves to the first three months of pregnancy, its effectiveness in animals with genital tract infection or acyclic cattle, and its ability to yield a higher number of embryos within the same period compared to the Multiple Ovulation and Embryo Transfer (MOET) technique. Understanding and improving the technical and biological factors influencing the OPU procedure is necessary to increase and optimize donor animal use in IVP and the number of quality oocytes obtained. This review aims to examine the specifics of the OPU approach and the factors influencing its performance in light of contemporary literature, as well as to propose fresh ideas to researchers.