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    Effects of supplementing a direct-fed microbial containing Enterococcus faecium 669 on performance, health, and metabolic responses of Holstein calves
    (Elsevier Science Inc, 2023) Biricik, Hakan; Brav, Fahir C.; Cetin, Ece; Aydin, Levent; Fantinati, Paolo; Cappellozza, Bruno I.
    This study aimed to evaluate the effects of Enterococcus faecium 669 supplementation on performance, health, parasitological, microbiological, and hematological responses of preweaning dairy calves. Forty-two newborn Holstein female calves (initial body weight [BW] 44 +/- 4.5 kg) were used in the present study. At birth, calves were ranked by initial BW and assigned to 1 of 2 treatment groups: (1) whole milk (CON; n = 21) and (2) whole milk with the addition of direct-fed microbial Ent. faecium 669 (DFM; n = 21). During the entire experimental period (63 d), DFM was daily-fed at a rate of 2.5 x 109 cfu/head. All calves were offered a mixture of a starter feed and wheat straw for ad libitum consumption. Supplement intake was evaluated daily, whereas calves were weighed on a weekly basis from d 0 to weaning (d 63). Diarrhea was assessed once a day, and fecal and blood samples were collected for microbiological, parasitological, and hematological responses. All data were analyzed with SAS using calf as the experimental unit. A treatment x week interaction was observed for BW, as DFM-supplemented calves were heavier than CON cohorts on d 56 (+ 4.7 kg) and at weaning on d 63 (+ 4.8 kg). A similar interaction was observed for average daily gain (ADG) and dry matter intake (DMI), with greater ADG for DFMsupplemented calves from d 35 to 42, greater ADG and DMI from d 49 to 56, and greater DMI from d 56 to weaning. Moreover, diarrhea occurrence tended to be lower, whereas rectal temperature was 0.2 degrees C lower for DFM-supplemented calves. Treatment x day interactions were observed for the occurrence and counts of Eimeria spp., as DFM-supplemented calves tended to have a reduced number of positive observations on d 42 of the study versus CON, and a significant reduction in positive animals from d 21 to 42 was observed in the DFM group but not in CON calves. For Cryptosporidium spp., no treatment effects were observed on overall occurrence (%), but DFM-supplemented calves had a greater count of oocyst per gram versus CON. No treatment x day interaction or main treatment effects were observed for any of the blood variables analyzed herein, exception being monocytes concentration. In summary, preweaning Ent. faecium 669 supplementation improved performance, diarrhea occurrence, and reduced the number of calves positively-detected for Eimeria
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    Influence of Genotype and Housing Systems on the Incidence of White Striping, Proximate Composition, and Sensory Analysis of Broiler Breast Meat
    (Aves, 2023) Ozbek, Melahat; Petek, Metin; Cetin, Ece; Cetin, Ismail
    This study was carried out to investigate the influence of genotype and housing systems on the incidence of white striping, proximate composition, and sensory analysis of broiler breast meat. In total, 10 random breast muscle samples of male broilers from each of 6 interactive groups (fast and slow-growing broilers x deep litter, fully slatted flooring and free-range housing; 2 x 3:6), slaughtered at 56 days old, were collected and analyzed in the study. The proximate composition analysis was evaluated using the AOAC Official Methods of Analysis. The occurrence of white striping was determined by examining the pectoral muscles of the broiler in the groups. A 9-point hedonic scale was used for sensory analysis of meat samples. The crude ash, crude fat content, and cooking loss values of the fast-growing broilers meat samples were found greater than slow-growing broilers' meat (p < .02, p < .001, and p < .033), while meat samples of slow-growing broiler had significantly higher crude protein and water-holding capacity values (p < .001 and p < .002). The crude fat content of free-range meat and crude protein of the meat produced from the slatted floors were significantly greater than the others (p < .001 and p < .043). The prevalence of white striping in breast meat in fast-growing broilers was significantly greater than that of slow-growing broilers. The meat produced from the slatted floor had significantly greater values for odor intensity, flavor intensity, and overall acceptability than the meat produced from free-range and deep litter housing (p < .012, p < .017, and p < .006). In conclusion, it can be said that the housing system, genotype, and genotype x housing system interactions affected the broiler's breast meat quality characteristics. All nutritional characteristics of broiler breast meat and the occurrence of white striping are significantly affected by genotype. Planning further research in commercial conditions should be more useful to see the comprehensive effects of the factors investigated in this experiment.