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  • Küçük Resim Yok
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    Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis
    (2023) Canbolat, Fadime; Kenanoglu, Nihan Akıncı; Yuksel, Tugba Nurcan; Berber, Ahmet Ali
    Valproic acid (VPA) is among the most commonly used antiepileptic drugs in childhood and adult epilepsy. Although VPA is well tolerated, it can cause life-threatening side effects. VPA has toxic and genotoxic effects. Antioxidants can reverse drugs' toxic and genotoxic effects. Therefore, our study aimed to evaluate the antigenotoxic protective effect of quercetin (QUE) against VPA genotoxicity by in vitro comet assay analysis method. Comet assay analysis was performed in five different groups. Group I; negative control (Sterile H2O), Group II; positive control (H2O2), Group III; VPA was applied in four different dose ranges, Group IV; QUE was applied in four different dose ranges, Group V; For the simultaneous combined administration of VPA and QUE, three different doses of VPA + four different doses of QUE were administered. Low-dose administration of QUE was more effective in ameliorating the damage caused by low-dose VPA (62.5 ?g/ml) administration. It is seen that the genotoxic damage caused by the application of 125 ?g/ml VPA can be eliminated by QUE at all doses. It was determined that different doses of QUE exhibited a significant antigenotoxic effect against damage caused by 125 µg/mL VPA (P<0.05). In our study, the curative effect of QUE on DNA damage was determined by in vitro comet analysis. Our analysis results showed that QUE ameliorates VPA-induced genetic damage.
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    Protective Effects of Ramelteon on Acute Lung Injury in Endotoxin-Induced Sepsis in Rats
    (2023) Yüksel, Tuğba Nurcan; Köse, Duygu; Gürbüz, Muhammet Ali; Halıcı, Zekai; Canbolat, Fadime; Bozgeyik, Esra
    Introduction: Sepsis is a life-threatening excessive systemic inflammatory reaction syndrome to infection that usually occurs in patients with bacteremia. The respiratory system is one of the structures most affected by acute organ damage. Melatonin plays an important role in re gulating various physiological functions of the body, including antioxidant and anti- inflammatory. Ramelteon (RAME) is the first melatonin receptor agonist confirmed for clinical use. The goal of this study is to determine the effects of RAME on endotoxin- induced septic lung injury in rats. Materials and Methods: Thirty-two female rats were separated randomly into four groups (n =8). Group healthy received intraperitoneal normal saline, group sepsis received intraperitoneally 10 mg/kg lipopolysaccharide (LPS), group sepsis+RAME2 received 10 mg/kg LPS plus 2mg/kg RAME, and group sepsis+RAME4 received 10 mg/kg LPS plus 4mg/kg RAME. RAME was administered by oral gavage 1 hour before LPS administration. The lung tissues were collected 12 hours after LPS administration and in vestigated molecularly (qRT- PCR analyses of Tumor Necrosis Factor-?, nuclear factor kappa-?, and interleukin 1-beta mRNA expression) and histopathologically (s taining with Harris Hematoxylin and Eosin Y). Results: TNF-?, NF- ??, and IL-1? levels significantly decreased dose-dependent in the septic rats following RAME administration. RAME admi nistration ameliorated histopathological injury in lung tissues due to sepsis. Conclusion: RAME ameliorated the inflammatory response in endotoxin-induced sepsis. These findings suggest that RAME can be a promising agent by contributing to alternative preventive treatment methods for sepsis with its anti- inflammatory effect.
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    Öğe
    Sıçanlarda Öngörülemeyen Kronik Hafif Stres Kaynaklı Depresyon Modelinde Propolisin Antidepresan Etkisinin Değerlendirilmesi
    (2023) Taşkıran, Ali; Canbolat, Fadime; Yucelli, Sena; Cevreli, Burcu
    Amaç: Bu çalışmada, sıçanlarda kronik öngörülemeyen depresyon modelinde propolisin antidepresan etkisi araştırıldı. Gereç ve Yöntem: Çalışmada Wistar-Albino erkek ratlar kullanıldı ve propolis, stres, stres+propolis, ve kontrol olmak üzere 4 gruba ayrıldı. Her gruba sekiz hayvan atandı. Deney protokolü stres gruplarına 60 gün süreyle uygulandı ve hayvanlar farklı stresörlere maruz bırakıldı. Propolis ekstresi (100 mg/kg) propolis ve stres+propolis gruplarına deney protokolü boyunca oral yoldan verildi. Depresyon modellemesi sonucunda davranışsal değerlendirme için Zorunlu Yüzme Testi, Sükroz Tercih Testi ve Yükseltilmiş Artı Labirent Testi uygulandı. Sıvı kromatografi-tandem kütle spektrometresi (LC-MS/MS) yöntemi ile idrarda serotonin (5-HT) ve metaboliti 5-hidroksi indol asetik asidin (5-HIAA) kantifikasyon analizi için 24 saatlik idrar örnekleri toplandı. Deney işlemi sonucunda hayvanlar sakrifiye edildi. Araştırmadan elde edilen sonuçlar Statistical Package for the Social Sciences kullanılarak analiz edildi. Bulgular: Gruplar arasında davranış testleri açısından istatistiksel olarak anlamlı fark olduğu görüldü (p<0,05). Sıçanlara stres altında propolis verilmesinin sıçanlarda şeker tüketimini değiştirdiği gösterildi (p<0,05). Zorunlu Yüzme Testi için, stres grubu ile diğer gruplar arasında istatistiksel olarak fark vardı. 5-HT ve 5-HIAA düzeyleri için gruplar arasında anlamlı fark yoktu (p>0,05). Sonuç: Bulgular, propolis özütünün antidepresan benzeri etkileri sayesinde depresyonu önlemeye yardımcı olabileceğini göstermiştir.