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Öğe Combining an ancient tradition with modern developments: Latin and open access(Int Assoc Plant Taxonomy-Iapt, 2013) Can, Levent; Erol, Osman; Şafak, Nevin; Küçüker, OrhanIn publishing names of new taxa of algae, fungi, and plants, it is argued that it is advantageous to retain the ancient tradition of Latin diagnoses in combination with the modern trend of publication in openly accessible electronic journals. Leading up to the XIX International Botanical Congress in 2017 in Shenzhen, China, we encourage that, even in the absence of a requirement, Latin continues to be used for diagnoses and consideration be given to making it a requirement that all valid publications of new taxa be freely accessible electronically. Retention of Latin diagnoses retains the discipline's link to the past and buffers against the rapid evolution modern languages are subject to, and Open access allows the discipline's results to be available to the widest possible audience.Öğe Crocus demirizianus sp nov from northwestern Turkey(Wiley, 2012) Erol, Osman; Can, Levent; Sik, LeventA new species, Crocus demirizianus O. Erol & L. Can is described and illustrated from northwestern Turkey. Crocus demirizianus was found in the Canakkale province, near the district of Can, and is undoubtedly related to the Crocus biflorus complex. However, the species differs from its relatives by typically sagittate anthers, and flowers without prominent stripes on outer segments. Diagnostic morphological characters are discussed and high resolution images are presented.Öğe Does insulin, transferrin and selenous acid preparation effect chondrocyte proliferation?(Turkish Assoc Orthopaedics Traumatology, 2014) Gökçe, Alper; Yılmaz, İbrahim; Gökay, Nevzat Selim; Can, Levent; Gökçe, ÇiğdemObjective: The aim of this study was to test the hypothesis that insulin, human transferrin, and selenous acid (ITS) preparation have positive effects on chondrocyte proliferation and morphology and investigate the biochemical and histological effects of these additive substances in different cell culture media. Methods: Human cartilage-derived cells (hCDCs) were isolated from the cartilage tissue of a 57-year-old woman diagnosed with gonarthrosis. Tissue samples were cultured in Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640. The cells' chondrogenic activities were observed. After serial passagings, cells were divided into 4 groups at the end of the 6th week. On the 14th day, proliferated cells were examined using an inverted microscope with x4, x10, x20 and x40 magnification and microphotographs were taken. Living cell quantity was determined on the first and 14th days using MTS-ELISA cell proliferation assay. Results: DMEM (without adding ITS premix solution) and RPMI-1640 containing ITS premix solution provide proliferation of the chondrogenic cells. The proliferation and viability of chondrocytes were revealed in this study in the 3rd group (DMEM solution without additives). Conclusion: It is suggested that the culture medium ingredients play crucial roles on chondrogenic proliferation in osteochondral tissue cultures.Öğe On the rediscovery of Euphorbia amygdaloides subsp. robbiae and its type(Tubitak Scientific & Technical Research Council Turkey, 2012) Can, Levent; Erol, Osman; Challen, Gill; Küçüker, OrhanEuphorbia amygdaloides L. subsp. robbiae (Turrill) Stace was first collected by Mrs Mary Anne Robb. She collected a wild growing Euphorbia L. specimen in 1891 from an unknown locality near Istanbul, and carried this plant in a bonnet box to England. Since 1891, this well-known cultivated plant has not been collected from any wild locality. This paper reports the wild specimens from Istanbul with morphological detail. We also present the composite image illustrations of the specimen, and images of the type specimen. The article also reveals that the date of the type specimen of Euphorbia amygdaloides subsp. robbiae does not match the literature, and it is proposed that the specimen dated 12 May 1949 be treated as the holotype. This paper explains why a lectotypification of the specimen is unnecessary.Öğe Phylogenetic and phylogeographic analysis of Myrmeleotettix maculatus (Orthoptera: Acrididae: Gomphocerinae) species group in Anatolia(Magnolia Press, 2021) Şirin, Deniz; Taylan, Mehmet Sait; Bircan, Rifat; Akyıldız, Gürkan; Can, LeventSix Anatolian and one European populations of the Myrmeleotettix maculatus species group, which contains M. maculatus and M. ethicus species, have been studied by using molecular genetics methods with mitochondrial COI gene. Myrmeleotettix ethicus is an Anatolian endemic species with local distribution whereas M. maculatus is distributed in western Palearctic. The phylogenetic analysis (ML and BI analyses) of the M. maculatus species group in Anatolia reveals that it consistently recovered two well-supported main clades and four different lineages. Molecular time estimates suggest that the diversification of the M. maculatus species group took place between the Late Tortonian (around 8-9 My) and the Middle of Pliocene-Pleistocene (around 4.3 My-present) periods and the current distribution of the genetic diversity has been affected by the uplifting of the Central Anatolian plateau, the termination of the Messinian salinity crisis, and the Quaternary climatic changes. Copyright © 2021 Magnolia Press.Öğe The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach(Tubitak Scientific & Technical Research Council Turkey, 2014) Erol, Osman; Kaya, Hilal Betul; Sik, Levent; Tuna, Metin; Can, Levent; Tanyolac, Muhammed BahattinIn this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents (2C) of the 26 Crocus specimens were found to range from 5.08 pg in C. asumaniae to 9.75 pg in C. sativus. Polymorphic information content (PIC) values were used to examine the capacity of the various primer pairs to amplify polymorphisms in the Crocus specimens. The PIC values ranged from 0.218 (M-CAA/E-AGC) to 0.512 (M-CAT/E-AAG) and showed an average of 0.34. In sum, we herein used AFLP analysis to identify a high level of polymorphism among Crocus specimens collected from various locations in Turkey and Greece, and our structural analysis yielded 2 reconstructed populations. These findings provide new insight into the relationships among different Crocus genotypes and show that AFLP analysis can be useful for Crocus diversity studies.Öğe Tritikale-Macar fiği hasılına enzim ve laktik asit bakterileri inokulant ilavesinin silaj kalitesi üzerine etkileri(Namık Kemal Üniversitesi, 2010) Can, LeventBu çalısma laktik asit bakteri inokulantları ve/veya enzimlerin, tritikale-Macar figi karısımı silajlarının fermantasyon, aerobik stabilite, hücre duvarı kapsamı ve in vitro organik madde sindirilebilirligi özellikleri üzerindeki etkilerinin saptanması amacı ile düzenlenmistir. Arastırmada kullanılan tritikale-Macar figi karısımı hasılları süt olum:çiçeklenme baslangıcı ve hamur olum:çiçeklenme sonu dönemlerinde hasat edilmistir. Laktik asit bakteri inokulantı olarak Inoculant-1188 (Pioneer®, USA), Enzim karısımı olarak Sil-All (Allteck, UK) kullanılmıstır. nokulantlar silajlara 6.00 log10 koloni form ünite/g düzeyinde katılmıslardır. Tritikale:Macar figi karısımları yalnızca gaz çıkısına olanak tanıyan, 1,0 litrelik özel kavanozlara silolanmıstır. Kavanozlar laboratuvar kosullarında 25±2°C'de depolanmıslardır. Silolamadan sonraki 2, 4, 8 ve 45. günlerde her gruptan 3' er kavanoz açılarak silajlarda kimyasal ve mikrobiyolojik analizler yapılmıstır. Silolama döneminin sonunda açılan tüm silajlara 5 gün süre ile aerobik stabilite testi uygulanmıstır. Ayrıca bu silajların, in vitro organik madde sindirilebilirligi saptanmıstır. Sonuç olarak LAB inokulantı ve enzimler, tritikale:macar figi silajlarının fermantasyon özelliklerini artırmıs ancak aerobik stabilitelerini düsürmüstür. Laktik asit bakteri+enzim karısımı inokulant, silajların asit deterjanda çözünmeyen lif ve selüloz kapsamını düsürürken, in vitro organik madde sindirilebilirligini artırmıstır.
