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  • Küçük Resim
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    Development of SSR markers and analysis of diversity in Turkish populations of Brachypodium distachyon
    (Bmc, 2009) Vogel, John P.; Tuna, Metin; Budak, Hikmet; Huo, Naxin; Gu, Yong Q.; Steinwand, Michael A.
    Background: Brachypodium distachyon (Brachypodium) is rapidly emerging as a powerful model system to facilitate research aimed at improving grass crops for grain, forage and energy production. To characterize the natural diversity of Brachypodium and provide a valuable new tool to the growing list of resources available to Brachypodium researchers, we created and characterized a large, diverse collection of inbred lines. Results: We developed 84 inbred lines from eight locations in Turkey. To enable genotypic characterization of this collection, we created 398 SSR markers from BAC end and EST sequences. An analysis of 187 diploid lines from 56 locations with 43 SSR markers showed considerable genotypic diversity. There was some correlation between SSR genotypes and broad geographic regions, but there was also a high level of genotypic diversity at individual locations. Phenotypic analysis of this new germplasm resource revealed considerable variation in flowering time, seed size, and plant architecture. The inbreeding nature of Brachypodium was confirmed by an extremely high level of homozygosity in wild plants and a lack of cross-pollination under laboratory conditions. Conclusion: Taken together, the inbreeding nature and genotypic diversity observed at individual locations suggest a significant amount of long-distance seed dispersal. The resources developed in this study are freely available to the research community and will facilitate experimental applications based on natural diversity.
  • Küçük Resim
    Öğe
    Diploid Brachypodium distachyon of Turkey: Molecular and Morphologic Analysis
    (Springer, 2009) Filiz, Ertuğrul; Özdemir, Bahar Sogutmaz; Tuna, Metin; Budak, Hikmet
    Brachypodium distachyon is a model species for the grass family, Poaceae, which includes major cereal crops such as wheat and barley. The aim of this study were to assess morphological and phylogenetic relationships among diploid accessions of Brachypodium representing diverse geographic regions of Turkey based on Sequence related Amplified Polymorphism (SRAP) analyses. The similarity matrix indicated close relation among species used in the section using SRAP primer combinations, produced 156 fragment bands, of which 120 were polymorphic. Genetic distance ranged from 0.03 to 0.62. Plant genotypes were grouped into two major clusters based on SRAP analysis. There was a high level of diversity among the native diploid Brachypodium genotypes. These genotypes can be used for a better understanding of grass genomics.