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  • Küçük Resim
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    Astragaloside IV and cycloastragenol production in in vitro produced Astragalus trojanus Stev.
    (Natl Inst Science Communication-Niscair, 2015) Nartop, Pınar; Gürel, Aynur; Akgün, İsmail Hakki; Bedir, Erdal
    In the present paper, an efficient method for clonal propagation of an endemic plant, Astragalus trojanus Stev., was described. Effect of sucrose and 6-benzyladenine (BA) concentrations on shoot proliferation and rooting was studied. Woody plant medium (WPM) supplemented with two different concentrations of BA (S1 medium with 0.5 mg/L BA & S2 medium with 1 mg/L BA) was used, where S2 medium resulted in higher rates in both shoot elongation and multiple shoot proliferation of clonally propagated A. trojanus shoot cultures. Of 27 clones, 3 were selected for further studies based on the highest regeneration capacity (Clones 1, 12 & 15). A total of 340 shoots were obtained from Clone 1 in S1 medium. In this medium, the rooting ratio of shoots for Clone 15 was 11.67%. The highest shoot elongation (1.99 cm), multiple shoot proliferation ratio (4.35) and multiple shoot proliferation percentage (91.67%) were obtained from Clone 1 in S2 medium. Two media, WPM medium supplemented with 15 g/L (K1 medium) and 30 g/L (K2 medium) sucrose were used for rooting. The root formation of Clone 1 was 26.67% in K2 medium. The highest astragaloside IV (0.5486 mu g/mg) and cycloastragenol (0.1263 mu g/mg) contents were detected in roots grown in K1 medium.
  • Küçük Resim
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    Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate
    (2019) Nartop, Pınar; Gürel, Aynur; Akgün, İsmail Hakkı; Bedir, Erdal
    In this study, two grams of callus regenerated from stem and leaf explants of Astragalus trojanus Stev. were cultured in Woody Plant Medium (WPM) supplemented with 1 mg/L 2,4-D for four weeks and used as inoculum in order to investigate theeffects of working volume and media composition. The highest biomass was obtained in 250 mL flask with astragaloside IV(1.66 µg/mg) and cycloastragenol (0.19 µg/mg) accumulation. Different concentrations of sucrose and casein hydrolysate (1and 2 g/L) were also tested and the effect of pH was also investigated. Biomass accumulation cannot be enhanced, however,astragaloside IV and cycloastragenol content was ascended. The highest astragaloside IV (95.23 µg/mg) and cycloastragenol(5.93 mg/mg) accumulations were obtained at pH 6.8 and 2 g/L casein hydrolysate, respectively
  • Küçük Resim
    Öğe
    Optimization of Precursor and Elicitor Utilization in Batch Cultures of Astragalus trojanus Stev.
    (2018) Nartop, Pınar; Gürel, Aynur; Akgün, İsmail Hakkı; Bedir, Erdal
    Elicitor and precursor applications are commonly used to induce secondary metabolism in plant cell cultures. In this study, methyljasmonate, jasmonic acid, salicylic acid and pectin were used as elicitors and ?-sitosterol was used as a precursor in batch cultures ofAstragalus trojanus in order to trigger astragaloside IV and cycloastragenol productions. Growth parameters (fresh and dry weights anddry weight percentages) of batch cultures were also evaluated in order to understand the effects of elicitors and precursor on primarymetabolism. All elicitors and precursor used in this study triggered metabolite production at different stages of culture period. Thehighest astragaloside IV accumulation (0.9435 µg/mg) was detected in medium supplemented with 50 µM methyl jasmonate at the 14thday of culture period, whereas the highest cycloastragenol concentration (0.3626 µg/mg) was found in medium supplemented with 50µM jasmonic acid at the 28th day of culture period. Large scale cultivation was also performed and 0.3759 µg/mg astragaloside IV was detected in medium supplemented with 50 µM methyl jasmonate at the 14th day.