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Öğe APJ receptor A445C gene polymorphism in Turkish patients with coronary artery disease(E-Century Publishing Corp, 2015) Akçılar, Raziye; Yümün, Gündüz; Bayat, Zeynep; Donbaloğlu, Mehmet Okan; Erselcan, Kubilay; Ece, Ezgi; Genç, OsmanCoronary artery disease (CAD) is a disease in which a waxy substance called plaque builds up inside the coronary arteries. Apelin is a novel endogenous peptide with inotropic and vasodilatory properties and is the ligand for the angiotensin receptor-like 1 (APJ) receptor. We aimed to determine genotype and allele frequencies of APJ receptor A445C gene polymorphism in Turkish patients with CAD and healthy controls by RFLP-PCR. This study was performed on 159 unrelated CAD patients and 62 healthy controls. We obtained AA, AC and CC genotype frequencies in CAD patients as 41.5%, 49.1% and 9.4%, respectively. In the control group, frequencies of genotypes were found as 35.5% for AA, 48.4% for AC and 16.1% for CC. We did not observe difference in APJ receptor A445C polymorphism between CAD patients and healthy controls (chi(2) = 2.178; df = 2; P = 0.336). The A allele was encountered in 66% (210) of the CAD and 59.7% (74) of the controls. The C allele was seen in 34% (108) of the CAD and 40.3% (50) of the controls. Allele frequencies of interested genes were not significantly different between groups (chi(2) = 1.57; df = 1; p = 0.225). The frequencies of APJ receptor A445C genotype were not significantly different between control and patients. None of the three APJ receptor A445C genotypes, AA, AC and CC displayed significant difference in CAD patients. We did not find any difference in the clinical parameters except for weight and diastolic blood pressure levels in the AA, AC and CC genotypes of patients. Individuals with CC genotypes had significantly higher weight, systolic and diastolic blood pressure levels and systolic blood pressure than other genotypes, P <= 0.05. In addition, HDL-C level was found decreased, but this reduction was not statistically significant. Contrarily, the low levels of weight, SBP, DBP and TC were statistically significant in the subjects with AA genotype in CAD. In conclusion, CC genotype carriers may have more risk than other genotypes in the development of hypertension in CAD, but not AAgenotype carriers. We suggest that this polymorphism may not be a marker of CAD, but it may cause useful in function of the apelin/APJ system and may be a genetic predisposing factor for diagnostic processes and can be helpfull in finding new treatment strategies. We think that it is required to further comprehensive studies in order to make clear this situation in CAD.Öğe APJ receptor A445C gene polymorphism in Turkish patients with coronary artery disease (Meeting Abstract)(Wiley-Blackwell, 2016) Akçılar, Raziye; Yümün, Gündüz; Bayat, Zeynep; Donbaloğlu, Mehmet Okan; Erselcan, Kubilay; Ece, Ezgi; Genç, Osman[No Abstract Available]Öğe Association of APJ G212A gene polymorphisms with plasma APJ levels and coronary artery disease risk in Turkish population(E-Century Publishing Corp, 2016) Akçılar, Raziye; Yümün, Gündüz; Donbaloğlu, Mehmet Okan; Erselcan, Kubilay; Bayat, Zeynep; Genç, OsmanApelin and apelin receptor (APJ) are required for normal cardiovascular development. To investigate the relationship between the APJ G212A polymorphisms and plasma APJ levels and the risk of coronary artery disease (CAD), allele and genotype frequencies of the APJ G212A gene were analysed in were analysed in 152 Turkish patients with CAD and 110 controls by the PCR-RFLP method. The frequencies of genotype GG (57.2% vs 18.2%, P = 0.000, OR = 0.21, 95% CI 0.1 to 0.44) and frequencies of allele G (70.1% vs 46.8%, P = 0.000, OR = 2.65, 95% CI 1.84 to 3.83) at APJ G212A gene polymorphisms were significantly increased in CAD compared with controls. Although plasma APJ level of the patients with GG genotype was lower, It was not statistically significant. CAD patients had significantly lower plasma APJ levels as compared with controls (P = 0.018). On the basis of our results, patients with CAD seem to have lower APJ levels and higher APJ-212 GG genotype and G allele. These results suggested that the APJ G212A gene may be associated with risk for CAD and the APJ G212A gene may play a role in CAD in the Turkish population.Öğe Association of vaspin rs2236242 gene variants and circulating serum vaspin concentrations with coronary artery disease in a Turkish population(Wiley, 2021) Akçılar, Raziye; Yümün, Gündüz; Bayat, Zeynep; Donbaloğlu, Mehmet Okan; Gür, Özcan; Gürkan, Selami; Arık, ÖzlemCoronary artery disease (CAD) is the primary cause of death worldwide. Vaspin was a recently described adipokine, playing a protective role in many metabolic and cardiovascular diseases. This study aimed to assess the relation of serum vaspin levels and vaspin rs2236242 polymorphisms with CAD. The study included 105 healthy subjects and 105 CAD patients. Serum vaspin concentrations and vaspin rs2236242 polymorphisms were determined by enzyme-linked immunosorbent assay and polymerase chain reaction, respectively. There was a statistically significant difference between the genotypes of CAD patients (TT 26.7%, TA 71.4%, and AA 1.9%) and controls (TT 70.5%, TA 28.6%, and AA 1%;chi(2) = 40.3;df = 2;p = .000). The TA genotype increased the risk of CAD (odds ratio [OR] = 6.60; 95% confidence interval [CI] = 3.60-12.1;p = .000) as compared to the TT genotype. There was a statistically significant difference between the allelic distribution of CAD patients (T 62.4% and A 37.6%) and controls (T 84.8% and A 15.2%;chi(2) = 27.0;df = 1;p = .000). Those carrying the A allele had a higher risk of CAD compared to those with the T allele (OR = 3.35; 95% CI = 2.10-5.36;p = .000). The serum vaspin concentrations of the patients with TT, TA, and AA genotypes were 30.4 +/- 1.72, 28.4 +/- 2.89, and 36.4 +/- 6.38 pg/ml, respectively, and there was no significant difference between the serum vaspin levels and vaspin genotypes (p = .696). All of the above suggested that the vaspin rs2236242 polymorphism was associated with CAD in the Turkish population.Öğe Characterization of the apelin-1860T>C polymorphism in Turkish coronary artery disease patients and healthy individuals(E-Century Publishing Corporation, 2015) Akçılar, Raziye; Yümün, Gündüz; Bayat, Zeynep; Donbaloğlu, Mehmet Okan; Erselcan, Kubilay; Ece, Ezgi; Genç, OsmanTo evaluate the association between the apelin -1860T>C polymorphism and plasma apelin levels in Turkish patients with coronary artery disease (CAD). A total of 276 individuals were enrolled in the present study, including 158 patients with CAD and 118 individuals without CAD as controls. The presence of the apelin -1860T>C gene polymorphism and plasma apelin levels were determined using polymerase chain reaction/restriction fragment length polymorphism and enzyme-linked immunosorbent assay, respectively. Significance was set at p?0.05 for all statistical analyses. The genotype and allele frequencies of interested genes were significantly different between groups (?2=10.2; df=2; p=0.006 and ?2=13.4; df=1; p=0.000, respectively). Frequency of CC genotype and the C allele of -1860T>C site was significantly higher in CAD patients compared to healthy controls. We found that individuals with the TC and CC genotypes were associated with an increased risk of CAD when compared with the TT genotype in CAD patients, and the adjusted ORs (95% CI) were 6.50 (1.27-33.0) and 6.39 (1.77-23.0), respectively. Plasma apelin levels were significantly lower in CAD patients compared to control group. Apelin level of CAD patient group having CC genotype of -1860T>C site was significantly lower compared to those having TT genotypes, but it was not statistically significant (p > 0.05). The homozygous CC genotype of apelin gene is associated with high risk of CAD. Apelin gene polymorphism -1860T>C is a significant predictor of predisposition to CAD in in Turkish population. © 2015, E-Century Publishing Corporation. All rights reserved.Öğe Hyperbaric oxygen treatment ameliorates lung injury in paraquat intoxicated rats(E-Century Publishing Corp, 2015) Akçılar, Raziye; Akçılar, Aydın; Şimşek, Hasan; Koçak, Fatma Emel; Koçak, Cengiz; Yümün, Gündüz; Bayat, ZeynepParaquat (PQ) is an agrochemical agent commonly used worldwide, which can cause acute lung injury (ALI) and death. Hyperbaric oxygen treatment (HBOT) is a therapeutic method, but the mechanisms of the protective effect of HBOT on ALI remain elusive. The purpose of this study was to evaluate the effect of HBOT on acute lung injury induced by PQ in rats. Wistar Albino rats (n=21) were separated into three groups of seven animals each: control (C), PQ, and PQ + HBOT groups. 20 mg/kg PQ was administered intraperitoneally in PQ and PQ + HBOT groups to induce experimental lung injury. Three days after PQ treatment, PQ + HBOT group was administered 100% O-2 at 2.0 ATA for 1 hour per day, for five consecutive days. At the end of the study, lung tissue was obtained for determining total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI) and histopathological determination. Tumor necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6), basic fibroblast growth factor (bFGF), transforming growth factor (TGF)-beta 1 mRNA levels were assessed by quantitative reverse transcription-polymerase chain reaction. In addition, the inducible nitric oxide synthase (iNOS) level in the plasma was determined. Plasma iNOS, OSI, tissue TNF-alpha, TGF-beta 1 and bFGF mRNA levels, and histological injury scores in PQ + HBOT group were significantly lower than PQ group. TAS level in PQ + HBOT group was significantly higher than PQ group. The findings suggest that HBOT could effectively ameliorate PQ-induced lung injury in rats.Öğe The Antioxidant Effect of Hyperbaric Oxygen Treatment on Paraquat-Induced Acute Lung Injury in Rats(Wiley-Blackwell, 2015) Akçılar, Raziye; Akçılar, Aydın; Şimşek, Hasan; Koçak, Fatma Emel; Koçak, Cengiz; Yümün, Gündüz; Bayat, Zeynep[No Abstract Available]
