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Öğe Association of Pb, Cd, and Se Concentrations and Oxidative Damage-Related Markers in Different Grades of Prostate Carcinoma(Humana Press Inc, 2012) Güzel, Savaş; Kiziler, Lebriz; Aydemir, Birsen; Alıcı, Bülent; Ataus, Süleyman; Aksu, Abdullah; Durak, HaydarProstate cancer is known to be affected by the heavy metal levels and oxidative damage of the body, yet there are very few studies which look into the way it occurs. The aim of this study was to determine whether blood and tissue lead (Pb), cadmium (Cd), and selenium (Se) levels are associated with oxidative damage in the context of prostate cancer progression and development. Seventy-nine patients comprising 25 patients with benign prostatic hypertrophy (BPH), 23 patients with malignant prostatic carcinoma (malign Ca), 16 patients with low-grade prostatic intraepithelial neoplasia (LGPIN), and 15 patients with high-grade prostatic intraepithelial neoplasia (HGPIN) diagnosed on the basis of their clinical profile, transrectal ultrasonography, and histopathology were included in this study. Cd and Pb levels in whole blood were found to be increased in patients with HGPIN compared with the BPH group; also, the levels of Cd in whole blood and tissue were found to be increasing in patients with malign Ca, unlike BPH patients. Moreover, the levels of malondialdehyde (MDA) in plasma and tissue were significantly increased in malign Ca, LGPIN, and HGPIN than those in BPH. However, the levels of tissue Pb were found to be decreasing in BPH, unlike the malign Ca and HGPIN patients, and the levels of tissue protein carbonyls in malign Ca were significantly lower than those in HGPIN. The levels of tissue reduced glutathione (GSH) in malign Ca were significantly lower than those in BPH. Additionally, the levels of Se in serum and tissue in LGPIN were significantly lower than those in BPH. The serum Se levels in HGPIN were also significantly lower than those in BPH and malign Ca groups. Furthermore, the concentrations of serum Se in LGPIN were significantly lower than those in malign Ca. From the Pearson correlation analysis, there were significant positive correlations between tissue Cd and MDA levels in malign Ca, LGPIN, and HGPIN and between the tissue Pb and tissue MDA and protein carbonyl levels in malign Ca. Blood Pb and tissue Pb were also significantly positively correlated with plasma MDA and protein carbonyl levels in malign Ca. In addition, blood Pb was significantly positively correlated with tissue MDA and protein carbonyl levels in malign Ca, and a significant positive correlation was also found between blood Cd and plasma protein carbonyls and tissue MDA in LGPIN. We observed that altered prooxidant-antioxidant balance and heavy metal levels may lead to an increase in oxidative damage and may consequently play an important role in prostate carcinogenesis. These findings indicate that changes in the levels of Pb, Cd, Se, MDA, protein carbonyls, and GSH in the blood and/or tissue are related to the prostatic carcinoma development and progression, although triggering one of the mentioned changes is unknown; therefore, further study is required to determine the exact steps of the process and clarify the roles of different substances in order to obtain a more detailed explanation of the phenomenon.Öğe Association of selenoprotein W1 (rs3786777) polymorphism, maternal plasma selenoprotein W (SelW), and selenium levels in patients with pre-eclampsia(Dustri-Verlag Dr Karl Feistle, 2019) Cinemre, Fatma Behice Serinkan; Cinemre, Hakan; Erdoğan, Elif; Dilaveroğlu, Nilgün; Tüten, Abdullah; Kaya, Baris; Aydemir, Birsen; Kızıler, Ali RızaObjective: To investigate the role of selenoprotein W1 (SEPW1) single nucleotide polymorphism (SNP) in etiopathogenesis of pre-eclampsia (PE) and its association with maternal selenoprotein W (SelW) and selenium levels. Materials and methods: In this study, 98 pregnant women who were diagnosed with PE and 100 healthy pregnant controls were investigated. To identify the polymorphism of the SEPW1 gene (rs3786777), allele-specific polymerase chain reaction (ASPCR) analysis was used. Serum selenium levels and plasma SelW levels were measured by graphite-furnace atomic absorption spectrophotometry and by ELISA, respectively. Results: Maternal selenium levels (mu g/L) were 92.56 +/- 6.10 and 86.26 +/- 6.33 in pregnant women with and without PE, respectively (p > 0.05). On the other hand, SelW levels (ng/mL) were significantly lower in PE (72.08 +/- 8.10) compared to controls (89.29 +/- 6.99) (p < 0.01). The frequencies of the CC, CA, and AA genotypes were found to be 26%, 61%, and 13% in pregnant women with PE and 28%, 55%, and 17% in healthy pregnant controls. The distribution of the SEPW1 genotypes and alleles did not differ significantly among subjects with and without PE. In PE patients, SelW levels were lower in CC and CA genotypes compared to controls (p < 0.05 and p < 0.001). Conclusion: SEPW1 gene polymorphism did not seem to affect risk of PE in our population. However, SelW levels were low in some genotypes of the gene, suggesting that SelW might have played a role in the etiopathogenesis of PE.Öğe Calcium dobesilate may improve hemorheology in patients undergoing coronary artery bypass grafting(2012) Beşirli, K.; Aydemir, Birsen; Arslan, C.; Kızıler, Ali Rıza; Cantürk, E.; Kayhan, B.Background: Calcium dobesilate is an angioprotective agent that has positive effects on hemorheological parameters. It is an antioxidant that increases endothelialderived vasodilator substance secretion, there are none that analyze its effects during the postoperative period of patients undergoing myocardial revascularization. Objective: We aimed to determine the effects of calcium dobesilate on hemorheological parameters, such as reduced glutathione and malondialdehyde in patients with ischemic heart disease undergoing myocardial revascularization in the postoperative period. Methods: One hundred and thirty-four patients operated for coronary heart disease were included in this study. Hemorheological, oxidant and antioxidant parameters were measured two days after surgery and after a period of treatment with calcium dobesilate. Then, 500 mg of calcium dobesilate was given twice a day to one group of 68 patients for three months. The control group was composed of 66 patients who did not receive this medication. Results: The increase in the erythrocyte deformability index was found to be significant compared with both the pretreatment values and with the 1st and 2nd values of the control group after calcium dobesilate administration, whereas there were no significant changes in blood viscosity, glutathione (GSH) or malondialdehyde (MDA) values after the calcium dobesilate administration. The same improvement in the CCS class was observed in patients regardless of they received the calcium dobesilate treatment. Conclusion: In the present investigation, the same improvement in the CCS class was observed in patients regardless of they received the calcium dobesilate treatment. Improvements with calcium dobesilate were statistically significant only in the increase in erythrocyte flexibility.Öğe Comparison of Before and After Varicocelectomy Levels of Trace Elements, Nitric Oxide, Asymmetric Dimethylarginine and Malondialdehyde in the Seminal Plasma and Peripheral and Spermatic Veins(Humana Press Inc, 2015) Kızıler, Ali Rıza; Aydemir, Birsen; Güzel, Savaş; Yaman, Ali; Gulyasar, Tevfik; Malkoc, Ercan; Acar, Ayşe; Yazıcı, Cenk MuratIncreased oxidative damage has been suggested to play an important role in the spermatogenesis and sperm function changes in patients with varicocele. However, changes in levels of nitric oxide (NO), asymmetric dimethylarginine (ADMA), malondialdehyde (MDA), iron (Fe), zinc (Zn) and copper (Cu) in blood and seminal plasma, and semen quality, are unknown. The aim of this study was to investigate the NO, ADMA, Fe, Cu, Zn and MDA levels from seminal plasma and peripheral and spermatic vein blood samples of patients with varicocele before and after varicocelectomy. In this before and after comparative study, 29 consecutive patients attending a training hospital in Tekirdag, Turkey, were recruited. MDA and NO levels were determined by spectrophotometric methods. The levels of ADMA were determined by enzyme-linked immunosorbent assay method. Trace element level was determined by atomic absorption spectrophotometric method. The levels of MDA in the seminal plasma and peripheral and spermatic vein samples were observed to decrease significantly in the comparison of before and after phases of the study group (p= 0.022, p= 0.001 and p= 0.034, respectively). Also, the levels of NO in the seminal plasma and spermatic vein samples decreased significantly in the comparison of before and after phases of the study group (p= 0.025 and p= 0.001, respectively), while the levels of ADMA in seminal plasma and spermatic vein samples increased significantly in the comparison of before and after phases of the study group (p= 0.003 and p= 0.001, respectively). There were no significant differences in the levels of trace elements and sperm count (p> 0.05). Oxidative stress is significantly higher in the spermatic vein and seminal plasma samples of patients with varicocele before varicocelectomy. In conclusion, these events may be evaluated accordingly for the potentially beneficial treatment methods.Öğe EFFECT OF BREAST CANCER AND BREAST CANCER TREATMENT ON THE BLOOD SERUM CONCENTRATIONS OF TRACE ELEMENTS AND SELENOPROTEINS(Polish Society Magnesium Research, 2022) Öğüt, Selim; Bahtiyar, Nurten; Mordeniz, Cengiz; Cinemre, Fatma Behice; Aydemir, Birsen; Karaçetin, Didem; Bektaş, MuhammetTrace elements (TEs) playing critical roles in chemical events that occur at the cellular level in the body are necessary for biological processes in human health. The role of TEs and selenoproteins and their relationship with breast cancer (BC) have not been studied thoroughly and therefore remain relatively unknown. Our study aimed to investigate possible changes in the serum selenoproteins (Glutathione Peroxidase 1 (GPX1), Glutathione Peroxidase 6 (GPX6), Selenoprotein F (Sel-F), Selenoprotein H (Sel-H), Selenoprotein S (Sel-S), Selenoprotein V (Sel-V), Selenoprotein M (Sel-M)), and TEs (Se, Zn, Mn, Cu, and Fe) levels, and TEs ratios (Fe/Se, Fe/Zn, Fe/Mn, Cu/Se, Cu/Zn, and Cu/Mn) in patients with BC before and after treatment (surgery, radiotherapy, and chemotherapy), and to evaluate the results in the patient groups with healthy controls. A total of 35 patients with BC and 25 healthy subjects were included in the study. Blood samples were collected from the patient group on the day prior to treatment, and on the day treatment was completed. Serum GPX1, GPX6, Sel-F, Sel-H, and Sel-S levels were decreased in both before and after treatment groups compared to the control. The treatment of BC resulted in increasing the concentration of Sel-V compared to before treatment levels. The treatment of BC resulted in lowering serum Se, Zn, and Fe concentrations compared to before treatment levels. Also, serum Se, Zn, and Fe levels were decreased in both before and after treatment groups compared to the control. The ratios of Cu/Se, Cu/Zn, and Cu/Mn were increased after treatment compared to the values before treatment. Cu/Se and Cu/Zn ratios were increased, but Fe/Mn ratios were decreased after treatment compared to healthy control. This study indicates that changes in serum levels of TEs such as Zn, Mn, Cu, and Se, as well as their ratios and selenoproteins, may be related to the treatments of BC. Further studies are required to clarify the exact specific mechanisms involved in the status of TEs and selenoproteins in therapeutic strategies of BC.Öğe Evaluation of metallothionein-1, metallothionein-2, lipid peroxidation and trace elements status in the progression of the spontaneous mitral chordae tendineae rupture patients(Dustri-Verlag Dr Karl Feistle, 2016) Aydemir, Birsen; Cinemre, Fatma Behice Serinkan; Cinemre, Hakan; Kızıler, Ali Rıza; Akdemir, Ramazan; Vatan, Mehmet Bülent; Akkoyun, Dursun ÇayanObjective: The aim of the present study was to investigate whether copper, zinc and iron levels and copper/zinc, iron/zinc, iron/copper ratios in serum and erythrocytes are associated with lipid oxidation levels, metallothionein-1 (MT-1), and metallothionein-2 (MT-2) levels in plasma in the context of pathogenesis of the mitral chordae tendineae rupture (MCTR). Methods: 65 subjects who were identical in demographic characteristics' were selected for the study; 33 with MCTR patients, and 32 healthy control subjects. The levels of MT-1, MT-2, and malondialdehyde as measure of lipid oxidation, and trace elements were determined by enzyme-linked immunosorbent assay, spectrophotometric method, and inductively coupled plasma optical emission spectrometry, respectively. Results: Serum zinc levels were found to be lower in the patients' group when compared to control group. The copper and iron levels in serum, MT-1, MT-2; malondialdehyde levels in plasma, iron and malondialdehyde levels in erythrocytes; serum copper/zinc and iron/zinc ratios were found to be higher in the patients' group when compared to controls. Conclusions: We conclude that the changes of oxidant antioxidant system balance and trace element status may contribute to the etiopathogenesis of MCTR.Öğe Gestasyonel Diabetes Mellitusta Bazı Biyokimyasal Parametreler, Eser Element Düzeyleri Ve Lipid Peroksidasyonu Arasındaki İlişkiler(Namık Kemal Üniversitesi, Tıp Fakültesi, 2013) Kızıler, Ali Rıza; Aydemir, Birsen; Cinemre, F.Behice; Cinemre, Hakan; Gülyaşar, Tevfik; Tüten, Abdullah; Öncül, Mahmut; Açıkgöz, A.Serdar; Akdemir, Nermin; Erkorkmaz, Ünal; Korkmaz, Gülcan Güntaş; Uzun, HafizeAmaç Çalışmamızın amacı gestasyonel diabetes mellituslu (GDM) gebelerde ve sağlıklı gebelerde serum bakır (Cu), çinko (Zn), demir (Fe), rutin biyokimyasal parametreler ve plazma malondialdehit konsantrasyonlarının ölçümü ve bu parametreler arasındaki olası ilişkileri her iki grupta karşılaştırmaktır. Materyal ve Metod GDM olan 56 gebeden hasta grubu ile 60 sağlıklı gebeden oluşturulan kontrol grubunda plazma malondialdehit, kan şekeri, insülin, HbA1c, total kolesterol, trigliserit ve LDLkolesterol biyokimyasal yöntemlerle ölçüldü. Serum Fe, Cu ve Zn alev atomik absorpsiyon spektrofotometresi ile ölçüldü. Bulgular Kan şekeri, insülin, HOMA-IR, HbA1c, total kolesterol, LDL-kolesterol, plazma malondialdehit, serum Fe ve Cu konsantrasyonlarının GDM’li grupta kontrol grubuna göre anlamlı olarak yüksek olduğu saptandı. Ancak serum Zn konsantrasyonları GDM grubunda kontrol grubuna göre anlamlı olarak azaldığı görüldü. GDM hasta grubunda LDL-kolesterol ile serum Cu ve plazma malondialdehit arasında negatif bir korelasyon saptandı. Ancak serum Fe ile plazma malondialdehit arasında ise pozitif bir korelasyon olduğu görüldü. Sonuç Bulgularımız GDM’li hasta grubunda anlamlı olarak Zn düzeylerinin yetersizliğini, hiperglisemi, hiperlipidemi ve malondialdehitin artışını göstermektedir. Zn elementi takviyesi antioksidatif sistemi desteklemesi ile antioksidatif enzim aktivitesini arttırılarak fetal ve maternal komplikasyonlarda iyileşme beklenebilir.Öğe Hipertansif Hastalarda Kognitif Fonksiyonların Değerlendirilmesi ve Serum Midkine Düzeyleriyle İlişkisi(2017) Güzel, Eda Çelik; Serinkan Cinemre, Fatma Behice; Güzel, Savaş; Kuçukyalcin, Volkan; Kızıler, Ali Rıza; Çavuşoğlu, Coşkun; Aydemir, BirsenAmaç: Hipertansiyon (HT) demans ile ilişkili risk faktörlerinden biridir. Çalışmanın amacı, HT'lu hastalarda kognitif fonksiyonlar ve serum midkine düzeyleri ile ilişkisini araştırmaktır. Gereç ve Yöntem: Bu çalışmada, 60 yaşın üstünde, en az beş yıllık eğitim alan ve HT tanısı almış 45 hasta ve 30 sağlıklı kontrol incelendi. Hastalara Mini Mental Durum Değerlendirme (MMDD) testi uygulanmıştır. MMDD skoru 24 ve daha düşük olanlar kognitif bozukluk olarak tanımlandı. Bu gruplarda serum midkine seviyeleri değerlendirildi. Bulgular:HT'lu hastalarının MMDD skorları kontrol grubuyla karşılaştırıldı ve anlamlı olarak düşük bulundu (p <0.01). HT'lu hastalarda midkine düzeyleri (25.10 ± 8.16 ng/mL) kontrol grubuyla (19.59 ± 7.53 ng/mL) kıyaslandığında belirgin olarak yüksekti (p <0.01). HT hasta grubunda kognitif bozukluğu olanlar ve olmayanlar arasında Midkine düzeyleri karşılaştıldı. Kognitif bozukluğu olan HT hastalarında Midkine belirgin olarak daha yüksekti (p <0.05). Ayrıca Midkine düzeyleri ile MMDD skorları arasında anlamlı bir negatif korelasyon gözlendi (r = 0.558, p <0.01). Sonuç:Bu çalışma, HT'lu hastalarda artan serum midkine düzeyleri ile kognitif fonksiyon azalması arasında önemli bir ilişki olduğunu göstermiştir.Öğe LOX-1 gene variants and maternal levels of plasma oxidized LDL and malondialdehyde in patients with gestational diabetes mellitus(Springer Heidelberg, 2016) Aydemir, Birsen; Baykara, Onur; Cinemre, Fatma Behice Serinkan; Cinemre, Hakan; Tüten, Abdullah; Kızıler, Ali Rıza; Uzun, HafizePurpose The aim of this study was to investigate the relationships between the maternal levels of oxidized LDL (ox-LDL), lipid peroxidation marker malondialdehyde (MDA) and LOX-1 3'UTR188C/T and K167N single nucleotide polymorphisms in pregnant Turkish women with gestational diabetes mellitus (GDM). Methods 116 pregnant women with GDM and 120 healthy pregnant women from the same geographic region were included in the study. Polymerase chain reaction-based restriction analysis was used to identify 3'UTR188C/T and K167N polymorphisms of the LOX-1 gene. Plasma ox-LDL and MDA levels were determined by enzyme-linked immunosorbent assay and spectrophotometric method in all study subjects, respectively. Results Our results indicated that the distribution of the LOX-1 3'UTR188C/T and K167N genotypes and alleles did not differ significantly among subjects with or without GDM (p> 0.05). TT and NN genotype carriers are associated with some glucose metabolism parameters (p< 0.05). There were no significant differences among plasma ox-LDL and MDA levels with regard to LOX-1 3'UTR188C/T and K167N polymorphisms in GDM group and control subjects (p> 0.05). According to the combined genotype analysis of LOX-1 3'UTR 188 TT and K167N NN polymorphisms, plasma MDA and ox-LDL levels were significantly different between women with GDM and healthy subjects either with or without combined TT/NN genotype carriers (p< 0.001). Conclusions According to our results, ox-LDL and MDA levels were increased in GDM pregnant women and healthy pregnant women either with or without combined TT/NN genotype carriers, for our Turkish sample, these genotype carriers appear to be related with increased oxidative stress in patients with GDM.Öğe May the level and ratio changes of trace elements be utilized in identification of disease progression and grade in prostatic cancer?(Dustri-Verlag Dr Karl Feistle, 2010) Kızıler, Ali Rıza; Aydemir, Birsen; Güzel, Savaş; Alıcı, Bülent; Ataus, S.; Tuna, M. B.; Kılıç, M.Objective: The effects of the level and ratio changes in the trace elements have been reported previously in several diseases. The aim of this study was to determine whether those trace elements (Cu, Fe and Zn) can be used to distinguish among different histology grades of prostate cancer development and progression, and to assess the level changes of trace elements in serum and in tissues and copper-to-zinc and iron-to-zinc ratios in the serum and prostatic tissues of patients. Material and methods: 69 patients comprised of 23 patients with benign prostatic hypertrophy (BPH), 20 patients with malignant prostatic carcinoma (Malign Ca), 14 patients with low-grade prostatic intraepithelial neoplasia (LGPIN) and 12 patients with high grade prostatic intraepithelial neoplasia (HGPIN) diagnosed on basis of clinical profile, transrectal ultrasonography (TRUS) and histopathology, were included in this study. The levels of elements were determined by atomic absorption spectrophotometer. Results: The mean serum Cu levels in malign Ca were significantly higher than those seen in LGPIN, in HGPIN, and controls (p<0.001, p<0.01, p<0.001, respectively). The mean serum Cu/Zn ratios in malign Ca were significantly higher than those in BPH (p < 0.01), LGPIN (p < 0.01), HGPIN (p < 0.01), and controls (p<0.001). However, the mean serum Fe levels in controls were significantly lower than those in BPH, malign Ca, LGPIN and HGPIN (p<0.001 for each). The mean serum Fe/Zn ratio in controls were significantly lower than those in malign Ca and LGPIN (p < 0.001 for each). The mean tissue Cu levels in malign Ca were significantly higher than those in LGPIN (p < 0.01) and HGPIN (p < 0.05). However, the tissue Zn levels of malign Ca were significantly lower than those of BPH (p < 0.05), but similarly these differences were not statistically significant among malign Ca, LGPIN and HGPIN. The mean tissue Fe concentrations were significantly lower in LGPIN as compared to HGPIN (p<0.05). From Pearson correlation analysis, there were significant positive correlations between Cu/Zn and Fe/Zn ratios in serum, Fe/Zn in serum and Fe/Zn in tissue in HGPIN (r = 0.636, p < 0.05; r = 0.776, p < 0.01, respectively). Serum Cu/Zn was significantly positively correlated with serum Fe/Zn, tissue Cu/Zn and tissue Fe/Zn in malign Ca (r = 0.527, p < 0.05; r = 0.685, p < 0.01; r = 0.556, p<0.05, respectively). A significant positive correlation was also found between tissue Cu/Zn and tissue Fe/Zn in malign Ca (r = 0.639, p<0.01). Moreover, there was a significant positive correlation between serum Cu/Zn ratio and tissue Fe/Zn ratio in LGPIN (r = 0.755, p<0.01). However, there was a negativ correlation between tissue Cu/Zn ratio and serum Fe/Zn ratio in LG PIN (r = -0.695, p < 0.01). Conclusion: These findings indicate that changes of levels of Cu, Zn and Fe, and Cu/Zn and Fe/Zn ratios in the serum and/or tissue are influenced by the prostatic carcinoma development and progression. Therefore, further studies need to be performed to clarify the exact role of these disparities of trace elements that are rooted from and are affected by tumorigenesis or by the result of tumorigenesis.Öğe Midkine Levels and its Relationship with Atherosclerotic Risk Factors in Essential Hypertensive Patients(Wolters Kluwer Medknow Publications, 2018) Güzel, Savaş; Cinemre, Fatma Behice Serinkan; Güzel, Eda Çelik; Küçükyalçın, Volkan; Kızıler, Ali Rıza; Çavuşoğlu, Coşkun; Aydemir, BirsenBackground and Objectives: Hypertension (HT) is one of the risk factors associated with atherosclerosis. Midkine (MK) plays a role as a growth factor in various biologic and pathologic events. In some reports, MK expression has been shown to be linked with vascular smooth muscle proliferation and neo-angiogenesis in atherosclerotic vessels. The aim was to research relationship of MK serum levels with some atherosclerotic risk factors in hypertensive patients. Methodology: This study examined 60 patients with essential HT and 30 healthy controls. Serum biochemistry, including lipid profile, MK, Vitamin B 12, C-reactive protein, zinc and copper levels were obtained. Results: MK levels of the HT patients were significantly higher than the control group (24.8 +/- 6.8 ng/mL vs. 18.39 +/- 5.6 ng/mL, respectively, P < 0.01). Lipid profile parameters such as total cholesterol, triglyceride, low-density lipoprotein (LDL) were also significantly higher in HT patients (P < 0.021, P < 0.01, and P < 0.01, respectively). Zinc levels were 179.13 +/- 34.06 mu g/dL and 172.55 +/- 45.47 mu g/dL in the HT and control group, respectively. Serum MK levels were positively correlated with diastolic (r = 0.288, P < 0.05) and systolic blood pressures (r = 0.390, P < 0.002), and also with serum total cholesterol (r = 0.406, P < 0.002) and LDL cholesterol (r = 0.318, P < 0.015) levels. Furthermore MK was also negatively correlated with zinc and Vitamin B 12 levels (r = -0.298, P < 0.023, r = -0.334, P < 0.027, respectively). Conclusion: This study has demonstrated an important association between increased serum MK levels and risk factors of atherosclerosis such as HT, increased total and LDL cholesterol.Öğe One of the Factor Associated with Etiopathogenesis of Diabetes Mellitus: Intraerythrocyte Fluid Volume(İstanbul Gelisim University, 2024) Bahtiyar, Nurten; Aydemir, Birsen; Hacıoğlu, Burcu; Sevinç Afşar, Leyla; Savaş, Gamze; Köse, Fadime; Azmamedova, İnciAim: The aim of the present study was to investigate the levels of intraerythrocyte fluid volume, erythrocyte indices, and biochemical parameters and to evaluate the relationship between intraerythrocyte fluid volume and these parameters in patients with Diabetes Mellitus (DM) and healthy controls. Method: The study included 42 patients with DM and 40 healthy controls. Biochemical parameters were measured using an automated analyzer. Complete blood counts were performed using an automated hematology analyzer, and intraerythrocyte fluid volumes were measured using the microcentrifugation method. Results: Intraerythrocyte fluid volume, glycated hemoglobin (HbA1c), and glucose levels were higher in the patient group than in the control group, whereas mean corpuscular volume (MCV), potassium (K), and sodium (Na) values were lower in the patient group than in the control group. On the other hand, a negative correlation was found between intraerythrocyte fluid volume and mean corpuscular hemoglobin concentration (MCHC), magnesium (Mg), and K values in the patient group. Conclusion: Studies findings indicated that intraerythrocyte fluid volume may be an effective hemodynamic parameter in the etiopathogenesis of DM. In line with these data, it can be suggested that intraerythrocyte fluid volume is an important factor that should be considered in monitoring the progression of the disease.Öğe Oxidized Low Density Lipoprotein Receptor 1 3'UTR 188C>T Gene Polymorphism in Patients with Coronary Artery Bypass Grafting(Galenos Publ House, 2023) Bahtiyar, Nurten; Baykara, Onur; Hacioglu, Yalcin; Oner, Tuba; Cinemre, Fatma Behice; Aydemir, Birsen; Onaran, IlhanAim: Coronary artery disease (CAD) is a pathological process characterized by atherosclerotic plaque accumulation in the epicardial arteries. Inflammation and high lipid levels play a role in pathological changes in atherosclerosis. Besides traditional risk factors, genetic factors such as single nucleotide polymorphism (SNPs) can be involved in disease process. In this study, we aimed to evaluate the effects of oxidized low density lipoprotein receptor 1 (OLR1) 3'UTR188C>T gene polymorphism, C -reactive protein (CRP), and lipid status in patients with coronary artery bypass grafting (CABG). Materials and Methods: The study population consisted of 109 CAD patients who had undergone CABG, and 127 healthy controls. The OLR1 3'UTR188C>T polymorphism was genotyped using PCR-RFLP technique. Serum CRP, high -density lipoprotein -cholesterol (HDL-C), and low -density lipoprotein -cholesterol (LDL-C) levels were measured with an automatic biochemistry analyzer. Results: The distribution of the OLR1 3'UTR188C>T genotypes and alleles did not differ significantly between CAD patients with CABG and controls. Serum CRP levels were increased in patients compared to the control group (p<0.001), but HDL-C, and LDL-C levels were not different between two groups. Traditional risk factor such as cigarette smoking, alcohol use, family history, diabetes mellitus and hypertension were increased in patients compared to the control group (p<0.001, for each). The CRP levels were higher in patients with the TT, CT, and CC genotypes than in controls with the same genotypes (p<0.001, p<0.01, and p<0.05, respectively). Conclusion: OLR1 3'UTR 188C>T polymorphism may not be involved in susceptibility to atherosclerosis. However, traditional risk factors in atherosclerosis such as smoking, alcohol consumption, family history, hypertension, diabetes mellitus, and circulating CRP levels were increased in our CABG population. The evaluation of OLR1 3'UTR188C>T and different OLR1 SNPs may be useful for their single and combined effects in atherosclerosis.Öğe Sağlık Hizmetleri Meslek ve Sağlık Bilimleri Yüksekokullarındaki Öğrencilerin Elektromanyetik Alan Maruziyetinin Araştırılması(2018) Öğüt, Selim; Sevinç, Leyla; Serinkan Cinemre, Fatma Behice; Cinemre, Hakan; Bahtiyar, Nurten; Küçük Ataman, Buket; Aydemir, BirsenAmaç Günümüzde, teknolojik gelişmelerle birlikte elektromanyetik alanlara (EMA) maruziyet oldukça artmıştır. Bunun insan sağlığı üzerindeki etkileri üzerine çeşitli çalışmalar bulunmaktadır. Bu araştırmada amacımız, Sağlık Hizmetleri Meslek ve Sağlık Bilimleri Yüksekokulları öğrencilerinin yaşam alanlarında EMA'lara maruziyeti ve sağlıkları üzerine etkilerini araştırmaktı. ( Sakarya Tıp Dergisi 2018, 8(4):806-812 )Gereç ve YöntemlerBu çalışmaya 552 öğrenciye EMA maruziyeti ve bunun sağlık üzerine etkilerini sorgulayan bir anket uygulandı.Bulgular Çalışmaya katılan öğrencilerin %34,2’si erkek, %65,8’i kız ve yaş ortanca değeri 21’idi (min-max: 18-41). Katılımcıların %29’u baz istasyonlarına ve %19,4’ü yüksek voltaj hatlarına yakın oturmaktaydılar. %45,5'i günde 5 saatten fazla elektrik/ elektronik sistemlere maruz kalırken, %72,5’nin yatak odasında elektrikli/ elektronik cihazlar bulundurmaktaydı. %83,9'unun evinde internet ağı, %44’ünün evinde mikrodalga fırın olduğu belirlendi. Katılan öğrencilerin %74,3’ü yorgunluk hissinden şikâyetçi idi ve ki kare analiziyle bu şikayet, günlük elektrikli/ elektronik cihaz maruziyet süresi ile anlamlı bir ilişki gösterdi (x2: 0.332- P<0.016). Aynı şekilde yorgunluk hissi ve göz rahatsızlıklarının (% 27,4), yatak odasında elektronik cihaz bulunması durumuyla istatistiksel anlamlı ilişki gösterdi (x2: 7.630- P<0.006; x2: 11.832- P<0.008, sırasıyla). Diğer bir anlamlı ilişki de günlük bilgisayar kullanımı saat ile koku problemi (% 4) arasında tespit edildi (x2: 11.832- P<0.008). Sonuç Teknolojik sistemlerden yayılan elektromanyetik radyasyonun insanlar üzerindeki uzun vadeli etkisi belirsiz olsa da, bulgular elektromanyetik radyasyonun insanlarda bazı sağlık sorunlarına neden olabileceğini düşündürmektedir. Daha kesin sonuçlar için daha kapsamlı popülasyonlarda bilimsel çalışmalara ihtiyaç vardır.Öğe Serum Trace Elements in Type 2 Diabetes Mellitus(Carbone Editore, 2017) Atalay, Hande; Boyuk, Banu; Güzel, Savaş; Altay, Murat; Kızıler, Ali Rıza; Aydemir, BirsenStudies suggested that imbalances of several trace elements may play an important role in normal glucose and insulin metabolism. The aim of the present study was to evaluate changes in serum levels of copper, zinc, iron, and magnesium in patients with type 2 diabetes mellitus (T2DM) and their effect on glycemic control. Sixty female patients with T2DM and seventeen healthy subjects were enrolled in this study. Fasting plasma samples were obtained from the patient and control groups. Trace elements were studied using an atomic absorption spectrometer. Correlation analyses of trace elements with metabolic parameters were analyzed using Spearman's Rho correlation coefficient. T2DM patients had a significantly high fasting plasma glucose, glycated hemoglobin (HbA1C), and microalbuminuria levels (p< 0.05). Serum magnesium levels were significantly lower in patients with T2DM compared with the healthy controls (2.0 +/- 0.2 ng/mL vs. 2.3 +/- 0.2 ng/mL, p< 0.05). Serum copper levels showed a negative correlation with diabetes duration (r= -0.338, p= 0.011), and iron levels were negatively correlated with body mass index and C-reactive protein (r= -0.407, p= 0.009; r= -0.390, p= 0.017). Serum magnesium levels indicated a correlation with HbA1C and creatinine clearance (r= -0.371, p= 0.049; r= -0.462, p= 0.023), but no significant correlation was found with any of the other variables and zinc levels. The present study found low levels of magnesium, iron, copper, and zinc in women with T2DM, which supports a close relationship of the above trace elements with glucose metabolism. Low magnesium levels has been linked to poor glycemic control in T2DM; therefore, magnesium deficiency should be prevented in patients with diabetes.Öğe The association of lectin-like oxidized LDL receptor 1 (LOX-1) K167N and 3'UTR188CT polymorphisms with maternal plasma soluble LOX-1 levels and preeclampsia risk in Turkish population(Springer Heidelberg, 2015) Tüten, Abdullah; Aydemir, Birsen; Öncül, Mahmut; Kızıler, Ali Rıza; Açıkgöz, A.Serdar; Güntaş Korkmaz, Gülcan; Uzun, HafizeTo investigate the main effect of polymorphisms in genes involved in endothelial pathophysiological mechanisms, LOX-1 K167N and 3'UTR188CT single nucleotide polymorphisms (SNPs) in relation to preeclampsia (PE) risk and possible interactions between the gene polymorphisms and plasma oxLDL and soluble LOX-1 (sLOX-1) levels on PE in Turkish population. LOX-1 K167N and 3'UTR188CT polymorphisms were studied in 113 pregnant women with preeclampsia and 96 healthy pregnant women by the PCR-RFLP techniques. sLOX-1 and oxLDL levels were determined by enzyme-linked immunosorbent assay (ELISA) in all study subjects. Patients having LOX-1 3'UTR188CT (OR 3.55, 95 % CI 1.89-6.67, P = 0.001) or 3'UTR188CC (OR 3.04, 95 % CI 1.25-7.38, P = 0.012) genotype had a significantly higher risk of PE than those with 3'UTR188TT genotype. Also, patients having K167N KK (OR 2.73, 95 % CI 1.33-5.61, P = 0.005) genotype had a significantly higher risk of PE than those with K167N NN genotype. LOX-1 3'UTR188TT and LOX-1 K167N NN genotype carriers were associated with significantly increased serum sLOX-1 level (P = 0.001). We further investigated the potential combined effect of these polymorphic variants on risk of PE development. According to the combined genotype analysis of LOX-1 3'UTR188TT and K167N NN polymorphisms, sLOX-1 and oxLDL levels also showed significant differences between PE patients and controls with or without combined TT/NN genotype carriers. Our findings indicate that higher plasma sLOX-1 and oxLDL concentrations, and the LOX-1 3'UTR188C > T and LOX-1 K167N gene polymorphisms were significantly associated with risk of developing preeclampsia. Plasma sLOX-1 may be a potential therapeutic target in the treatment of preeclampsia.Öğe The Circulating Levels of Selenium, Zinc, Midkine, Some Inflammatory Cytokines, and Angiogenic Factors in Mitral Chordae Tendineae Rupture(Humana Press Inc, 2015) Aydemir, Birsen; Akdemir, Ramazan; Vatan, M. Bülent; Cinemre, Fatma Behice Serinkan; Cinemre, Hakan; Kızıler, Ali Rıza; Öğut, SelimChordae tendineae rupture process is associated with increased production of inflammatory and angiogenesis mediators in connective tissues, which contributes to chronic inflammation and pathogenesis of degenerative chordae. A few trace elements are known to possess antioxidant, anti-inflammatory, and antiangiogenic properties. Therefore, the aim of this study was to determine whether zinc, selenium, midkine (MK), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), vascular endothelial growth factor-A (VEGF-A), platelet-derived growth factor-BB (PDGF-BB), and reduced glutathione (GSH) levels are associated with inflammation and angiogenesis processes in the context of a potential etiology causing aggravation of mitral regurgitation and/or ruptured chordae tendineae. Seventy-one subjects comprising 34 patients with mitral chordae tendineae rupture (MCTR) and 37 healthy controls diagnosed on the basis of their clinical profile and transthoracic echocardiography were included in this study. The levels of GSH, MK, selenium, and zinc were found to be lower in the patients group when compared to control group. There were no significant difference in plasma TNF-alpha, IL-1 beta, IL-6, IL-8, VEGF-A, and PDGF-BB levels between two groups. There were positive significant correlations between MK and GSH, MK, and selenium levels in patients with MCTR. According to our data in which selenium, zinc, MK, and GSH decreased in MCTR patients, inflammatory response, oxidative stress, and trace element levels may contribute to etiopathogenesis of mitral regurgitation and/or ruptured chordae tendineae.Öğe The Clinical Efficiency of Minerasol (R) a New Trace Element Combination on Sheep(Medwell Online, 2010) Or, M. Erman; Kayar, Abdullah; Gönül, Remzi; Dokuzeylül, Banu; Kızıler, Ali Rıza; Aydemir, Birsen; Barutçu, BoraIn this study, the effects of Minerasol (R) application, is a new mineral combination, on blood serum parameters, its efficiency on sheep breeding and its safety on the treatments of diseases (caused by macro and micro element deficiencies) were examined. When the serum mineral level before and after the application of Minerasol (R) were examined on 40 sheep, it was determined that, there were increases in serum Fe, Cu, Zn, Mg and Ca levels. The increase in. serum Zn, Mg and Ca were significant (p<0.001). Consequently, it was concluded that with the inclusion of Minerasol (R) into the routine care and nourishment program, the problems related to the deficiency of trace elements would be eliminated in a short time and predisposition in terms of bacterial, viral and parasitic diseases would be prevented.Öğe The relationship between copper, homocysteine and early vascular disease in lean women with polycystic ovary syndrome(Taylor & Francis Ltd, 2013) Çelik, Cem; Bastu, Ercan; Abalı, Remzi; Alpsoy, Şeref; Güzel, Eda Çelik; Aydemir, Birsen; Yeh, JohnThis study investigates copper (Cu) levels and vascular dysfunction in lean women with polycystic ovary syndrome (PCOS). 44 subjects with PCOS, diagnosed according to Rotterdam criteria, and 42 healthy subjects matched for body mass index and age. Comparison of serum Cu, homocysteine, carotid intima-media thickness (CIMT), brachial artery flow mediated dilation (FMD) was carried out between PCOS patients and the control group. Clinical study was done in Namik Kemal University School of Medicine. The CIMT and concentration of Cu in PCOS patients was significantly higher than the healthy controls. FMD levels in PCOS patients were significantly lower than those in controls. In PCOS patients, CIMT was correlated with estrogen and Cu levels. However, FMD was correlated with age and Cu levels. Among these contributing factors, Cu levels were correlated with a change in CIMT and FMD. CIMT and FMD in PCOS patients were related to Cu levels as well as several cardiovascular risk factors. Thus, increased Cu levels may be responsible for the increased risk of early vascular disease in women with PCOS.Öğe The Role of Maternal Oxidative Stress, Iron/Zinc, Copper/Zinc Ratios and Trace Element Levels in the Pathogenesis of Preeclampsia(2017) Serinkan Cinemre, Fatma Behice; Cinemre, Hakan; Kartal, Nagihan; Gülyaşar, Tevfik; Yıldız, Mustafa; Tüten, Abdullah; Aydemir, BirsenPurpose:Preeclampsia (PE) is a complex disease and the underlying mechanisms are not known, yet. It is well known that oxidative stress and trace elements play a role in the pathogenesis of various diseases. Several studies have shown that the levels and proportions of trace elements are closely related to the severity of the disease. The aim of the study was to investigate the changes in some characteristics parameters, serum zinc, iron, copper levels, and copper/zinc and iron/zinc ratios and plasma lipid peroxidation levels in patients with mild and severe preeclampsia
