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Öğe Crossing of Alphonse lavallee and regent grape cultivars for downy mildew resistant genotypes. 1. seed germination and seedling growth(Centenary University, 2019) Uzun, Halil İbrahim; Özer, Nuray; Akkurt, Murat; Özer, Cengiz; Aydın, Serkan; Aktürk, BurakCrossing for disease resistance of grape cultivars is one of the main interests of grape breeding. Downy mildew is one of the destructive diseases of grapevines. V. vinifera has superior characteristics for wine or fresh consumption but mildew sensitive. Vinifera grapevines are cross with American wild grapes to obtain resistant cultivars. But some hybrid grapes have low seed germination. The main goal of this study was to develop hybrid table grape F1 offsprings with resistance to downy mildew disease and to test seed characteristics of them. Alphonse Lavalle (susceptible) and Regent (resistant) grape cultivars were crossed to obtain resistant F1 progenies. Hybrid seeds were soaked in gibberellic acid (GA3, 1 000 ppm), Benzylaminopurine (BAP, 1 000 ppm) and hydrogen peroxide (H2O2, 1 M) solutions and water (control) for 24 hours after stratification (4 months at 5oC) and then sown in Perlite: Peat moss (1:1) potting soil. Germination was carried out in the plastic boxes with constant temperature (27oC) and relative humidity (99%). Total germination ranged from 60.39% to 78.32% in the control and GA3 treated seeds respectively. Also, GA3 significantly increased total germination when compared to the other treatments. Optimization of growth chamber, greenhouse and open field conditions for seed germination and seedling growth accelerated shoot growth up to 2 m in offsprings at the first year. © 2019, Centenary University. All rights reserved.Öğe Marker assisted selection (MAS) for downy mildew resistance in grapevines using Rpv3.1 associated markers(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2022) Akkurt, Murat; Şenses, İrem; Aktürk, Burak; Tozlu, İlhami; Özer, Nuray; Uzun, Hail IbrahimPowdery mildew and downy mildew are primary fungal diseases that cause significant damage in viticulture. Therefore, breeding powdery and/or downy mildew resistance is one of the priority subjects in grapevine breeding programs. This study aims to conduct early-selection by marker assisted selection (MAS) method among 869 genotypes obtained through crossbreeding 'Alphonse Lavallee' x 'Regent' cultivars using the markers (GF18-06 and GF18-08) associated with downy mildew resistance gene region Rpv3.1 to develop new grapevine cultivars resistant to downy mildew caused by Plasmopara viticola. A total of 869 hybrid plants which were obtained after crossing 'Alphonse Lavallee' x 'Regent' in a 3-year breeding program were used in the study. The hybrid plants were scored for the resistance level based on their sporulation intensity after artificial inoculation of P. viticola. DNA samples of the hybrid plants were amplified with GF18-06 and GF1808 markers in Polymerase Chain Reaction (PCR) for MAS. The alleles which were associated to Rpv3.1 resistance locus and the results of resistance scoring were compared, and the applicability of the markers in MAS was verified. It was determined that the GF18-08/410 bp marker can be used successfully for MAS. Gf 18-06 marker 385 bp, 390 bp and 407 bp gave false positive results in our population, respectively 8.86%, 9.02% and 37.94%. Therefore, this may limit its use for MAS.Öğe Resistance assessment of grapevine leaves to downy mildew with sporulation area scoring(Springer Science and Business Media B.V., 2021) Özer, Nuray; Uzun, Halil İbrahim; Aktürk, Burak; Özer, Cengiz; Akkurt, Murat; Aydın, SerkanThe use of resistant individuals obtained through a breeding program, aimed at decreasing chemical treatments, is one of the most promising strategies for control of downy mildew caused by Plasmopara viticola in grapevine. In this study, 869 hybrid grape individuals, obtained by crossing the resistant Regent grape cultivar with sensitive Alphonse Lavallée, were tested after artificial inoculation of detached leaves by drops of the pathogen inoculum. Sporulation severity, as visual evaluation of sporulation density including necrosis, and sporulation incidence were scored to assess resistance level, then the sporulation area (mm2) was measured for each individual. The criterium of sporulation incidence could not describe all resistance levels. Sporulation area made it possible to measure the entire area in detail for how the pathogen spread at the inoculation site. Sporulation area was not fully consistent with sporulation severity. In this context, a new scale for sporulation area was developed to determine resistance level to the pathogen by taking into account the distribution of sporulation area values within those obtained from sporulation severity scales. Six categories ranging from 0 to 0.05 mm2 (extremely resistant-ER) to ?15.1 mm2 (extremely sensitive-ES) were defined. 43 of the 869 individuals tested through the three evaluation criteria, in which no sporulation was observed, and 18 additional individuals with sporulation area of <0.05 mm2 were scored in the ER group. Microscopic analysis confirmed findings for the ER group and other resistance levels. The authors suggest that the scale of sporulation area will be useful for assessment of resistance to downy mildew on grapevine leaves. © 2021, Koninklijke Nederlandse Planteziektenkundige Vereniging.Öğe Susceptibility to Downy Mildew (Plasmopara Viticola) and Powdery Mildew (Erysiphe Necator) of Different Vitis Cultivars and Genotypes(Estacao Vitivinicola Nacional, 2017) Atak, Arif; Akkurt, Murat; Polat, Z.; Çelik, H.; Kahraman, K. A.; Akgül, D. S.; Eibach, R.; Özer, NurayTurkey has a very old history of viticulture and also homeland of the grapevine (Vitis spp.). Vitis cultivars belonging to different species are grown in almost every region in the country. However, particularly downy mildew and powdery mildew diseases affect the cultivars belonging to Vitis vinifera. In northern of Turkey Vitis labrusca and hybrids between V. vinifera and V. labrusca are rather common. V. labrusca cultivars or genotypes exhibit generally higher degree of resistance than V. vinifera cultivars. However, resistance level can vary from cultivar to cultivar and even from clone to clone within one cultivar. In this study, different Vitis hybrids and genotypes which exhibit different downy and powdery mildew susceptibility are compared for two years. Especially some V. labrusca hybrids and genotypes appeared resistance for both diseases. On the other hand, interspecific crosses and V. vinifera cultivars were found to be more susceptible. Using resistant lines as parent in later breeding activities, it could be possible grow high quality table cultivars with much fewer pesticide applications or possibly without them.Öğe Üzüm Çekirdeklerinin Çimlendirilmesinde Etkili ve Pratik Bir Yöntem: Kutuda Çimlendirme(2018) Uzun, Halil İbrahim; Özer, Nuray; Akkurt, Murat; Özer, Cengiz; Aydın, Serkan; Aktürk, BurakAsma ıslahında en önemli engellerden biri, iki üzüm çeşidinin melezlenmesi sonucu elde edilen melezçekirdeklerin çimlenme oranının düşük olmasıdır. Çimlenmeyi etkileyen en önemli faktörler arasında;ortam ve tohum nemi gelir. Ayrıca büyümeyi düzenleyici maddeler (BDM)de, çimlenmeyi olumluetkilemektedir. Bu çalışmada, melez üzüm çekirdeklerine BDM uygulamak ve kapalı ortamlarda kararlı birnem düzeyinde çimlendirilmek suretiyle çimlenme oranını arttırmak amaçlanmıştır. Alphonse Lavalle ×Regent melezlemesinden elde edilen F? melez çekirdekleri, nemlendirilmiş perlitte 4 ay süreyle, +5?’dakatlamayı takiben; torf: perlit (1:1) harç ortamı içeren 5 cm çapındaki torf saksılara ekilmiştir. Çimlenmeyiteşvik etmek için, çekirdekler ekimden önce 24 saat gibberellik asit (GA?, 1000 ppm, benzil aminopurin(BAP, 1000 ppm), GA?+BAP (1000 ppm+1000 ppm), hidrojen peroksit (H?O?, 1 M) çözeltisinde ve suda(kontrol) tutulmuştur. Saksılar, bağıl nem miktarı sürekli %99 düzeyinde olan kapaklı plastik kutularakonulmuş ve bu kutular sıcaklığı 27? olan çimlendirme odalarına alınmıştır. Çimlenen çekirdekler yineplastik kutulara konularak sıcaklığı 25? olan ve kutuların hemen üstü LED lambalar ile aydınlatılmış(PAR=135–317 ?mol m?² s?¹) bitki gelişme odalarına yerleştirilmiştir. Kutuların kapakları her iki odada dakapalı tutulmuştur. Bitkicikler 3–5 gerçek yaprak oluşunca, plastik torbalara alınmış ve daha sonra ısıtmalıseraya alınmıştır. Uygulamalar sonucunda çimlenen çekirdek ve elde edilen bitki yüzdesi kontrolde %60.39iken, 1000 ppm GA? uygulanmış çekirdeklerde %78.32’ye kadar yükselmiştir. Üzüm çekirdeklerinin 24saat GA? çözeltisine batırılmasından sonra plastik kutularda çimlendirilmesi ve bunu takiben yine plastikkutularda geliştirilmesi, asma ıslahı çalışmalarında başarıyı arttıracak etkin bir yöntemdir.
