dc.contributor.author | Yılmaz, Remziye | |
dc.contributor.author | Cabi, Evren | |
dc.contributor.author | Doğan, Musa | |
dc.date.accessioned | 2022-05-11T14:28:31Z | |
dc.date.available | 2022-05-11T14:28:31Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0556-3321 | |
dc.identifier.issn | 2070-3368 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/6855 | |
dc.description.abstract | RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase Chain Reaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some species in the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used in the study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments which have been used as a measure of the degree of relationship between species by means of using the RAPD-PCR results with three primers selected for identifying the genetic similarities. Polymorphic melting profiles have been obtained with Post PCR MCA method using three primers. Genetic similarities are calculated for all the species studied with RAPD-PCR and MCA methods, the dendrograms are obtained with the MVSP (Multi Variate Statistical Package) software using UPGMA (Unweighted Pair Group Method with Arithmetic Averages) and Jaccard's Coefficient. Polymorphism between 18 populations of Eremopyrum and 6 Agropyron populations and within the species are determined by using RAPD-PCR and Post PCR melting curve analysis (MCA) respectively. | en_US |
dc.description.sponsorship | Scientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-TBAG-105 T 171] | en_US |
dc.description.sponsorship | We would like to thank Prof. Dr. Meral YUCEL, METU Central Lab., Molecular Biology and Biotechnology R&D Center, for providing all equipments used in this study. We also wish to thank to the Scientific and Technical Research Council of Turkey (TUBITAK-TBAG-105 T 171) for their financial assistance for the study. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Pakistan Botanical Soc | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Intergeneric Hybridization | en_US |
dc.subject | Tribe Triticeae | en_US |
dc.subject | Rapd Markers | en_US |
dc.subject | Taeniatherum | en_US |
dc.subject | Orientale | en_US |
dc.title | Molecular Analyses of the Genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae) By Pcr Methods | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Pakistan Journal of Botany | en_US |
dc.department | Fakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü | en_US |
dc.authorid | 0000-0003-0340-1195 | |
dc.authorid | 0000-0002-7706-5801 | |
dc.authorid | 0000-0003-2041-1205 | |
dc.identifier.volume | 46 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 769 | en_US |
dc.identifier.endpage | 774 | en_US |
dc.institutionauthor | Cabi, Evren | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.authorscopusid | 7004114724 | |
dc.authorscopusid | 33367486100 | |
dc.authorscopusid | 7102837994 | |
dc.authorwosid | Dogan, Musa/AAB-3970-2021 | |
dc.authorwosid | Cabi, Evren/H-2263-2019 | |
dc.authorwosid | Doğan, Musa/AAZ-9004-2020 | |
dc.authorwosid | YILMAZ, Remziye/M-8310-2018 | |
dc.identifier.wos | WOS:000338626100001 | en_US |
dc.identifier.scopus | 2-s2.0-84902006753 | en_US |