Choise of Explant Material and Media for in vitro Callus Regeneration in Sultana Grape Cultivar (Vitis vinifera L.)

Abstract

The study was carried out to choise of explant material and culture media for callus regeneration in ‘Sultana’ (Vitis vinifera L.). Leaf disc and node explants were the main explant materials which were taken from in vitro shoots which were obtained from macroshoot tip explants. The initial macroshoot tip were cultured on MS (Murashige and Skoog) medium including BAP (6-benzylaminopurine) (1 mg L-1 ) and in vitro shoots were subcultured on shoot multiplication medium with BAP (1 mg L-1 ) + IBA (Indole-3-butyric acid) (0.1 mg L-1 ) In order to investigate callus regeneration potential of Sultana grape cultivar, leaf disc explants were cultured on two different MS medium including BAP in combination with 2,4-D (2,4-dichlorophenoxyacetic acid) whereas node explants cultured on four different MS medium including BAP in combination with 2,4-D and NAA (naphthalene acetic acid). The intensity of callus proliferation was greater in leaf disc culture than in node culture. In all media combinations, MS medium including BAP (1 mg L-1 ) + 2,4-D (0.1 mg L-1 ) were found to be the most effective on callus regeneration. In this medium, callus regeneration rate was found to be 100% and the average diameter of callus was found to be 6.3 mm.