Effects of colchicine applications on viability and ploidy level in in vitro (solid medium) shoot tip culture of 'Michele Palieri' and 'Tekirdağ Misketi' grape cultivars

Abstract

The purpose of this study is achieved tetraploid grape lines for the first phase of polyploidy breeding. This study was carried out at Tekirdağ Viticultural Research Institute Tissue Culture Laboratory with ‘Michele Palieri’ and ‘Tekirdağ Misketi’ grape cultivars. Explants of 0.5-0.8 mm size were taken from Tekirdağ Viticulture Research Institute vineyards in late June-early July 2014. In this method, different concentrations of colchicine were added to the Murashige Skoog (MS) nutrient medium in in vitro culture. The explants, which were kept in the colchicine medium at different waiting times, were transferred to the MS nutrient medium without chemical mutagen. Explants were grown in a 16-h bright and 8-h dark photoperiod, with a temperature of 25±1°C, placed in shelves in a controllable climatic room provided with light at a lighting intensity of 3500 lx m-2. Results of this research were showed that grape cultivars may give different responses to chemical mutagens in in vitro shoot tip culture studies. The viability rate of ‘Tekirdağ Misketi’ decreased after application of chemical mutagen. This cultivar has been found to be a susceptible cultivar for colchicine applications. In the result of the flow cytometry analyses of leaf samples taken from plants, it was determined that diploid construction continued in all samples and the colchicine applications did not change the number of chromosomes. The DNA content of the samples was determined to be about 1.00 pg/2C diploid (2n) grapevine size. © 2020 International Society for Horticultural Science. All rights reserved.