Nucleotide Sequence Variations of Lactoferrin Gene in Anatolian Water Buffaloes

Abstract

The candidate gene approaches have been extensively used to identify the genes close to quantitative trait locus (QTLs) which is responsible for economically important traits in livestock animals. However, advances in molecular genetics have bring with high throughput sequencing technologies in recent years. And, these technologies have provided powerful method for QTL analysis. Lactoferrin is one of the significant candidate gene related to milk yield and quality in dairy animals. Furthermore, there were strong evidences that explain the significant associations of lactoferrin gene and mastitis disease in dairy animals. In this study, sequence analysis were performed to identify the lactoferrin gene polymorphisms in Anatolian water buffaloes. The sequence analysis were successfully revealed the seven single nucleotide polymorphism (SNPs) (g.1351G>A, g.1113A>G, g.1214C>T, g.1110A>G, g.1051A>G, g.1120C>T and g.1127C>T) in Anatolian water buffaloes. And, all of the SNPs were found in agreement with Hardy Weinberg equilibrium (P<0.05). However, the genotype analysis were also identified three haplotypes H1, H2 and H3. And, the frequencies of H1, H2 and H3 were determined as 0.350, 0.440 and 0.210, respectively. To the best of my knowledge, this is the first study that fully describes seven single nucleotide polymorphisms of lactoferrin gene in Anatolian water buffaloes.