Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method

Abstract

Leptin is a hormone which produces 16 kDa protein predominantly expressed in adipose tissue. Leptin gene has pleiotropic effects on several functional traits such as milk yield, carcass and meat quality, growth, feed intake and fertility. Therefore, this study was performed to identify single nucleotide polymorphism of bubaline leptin gene in Anatolian buffaloes. In this study, 513 bp of leptin DNA fragment was amplified. A PCR-RFLP analysis was used to identify bubaline leptin gene T1131G polymorphism. DdeI restriction enzyme was used to genotype bubaline leptin gene T1131G polymorphism in Anatolian buffaloes. The analysis revealed TT, GT and GG genotypes in Anatolian buffaloes. Allele frequencies of T and G were found 0.478 and 0.521, respectively. This study is the first report of identification of bubaline leptin gene T1131G polymorphism by using PCR-RFLP method in Anatolian buffaloes.